##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AccIII AgeI ApaI AscI
AspI AviII AvrII BamHI BciVI
BclI BglI BglII BlnI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI EagI EclXI Eco52I EcoRI
EcoRV FbaI FseI FspI HincII
HindII HpaI KspI MluI MroI
NcoI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PvuI
SacI SacII SalI SapI SbfI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 80 GGTACC G^GTACC
AccII 5 CGCG CG^CG
AclI 3939 AACGTT AA^CGTT
AflIII 1874 ACATGT A^C[GA][CT]GT
AhdI 4205 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4075 GTGCAC G^TGCAC
AlwNI 1241 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1557 AGCGCT AGC^GCT
ApaLI 4075 GTGCAC G^TGCAC
AseI 3557 ATTAAT AT^TAAT
AsnI 3557 ATTAAT AT^TAAT
Asp718I 80 GGTACC G^GTACC
AspEI 4205 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 164 GGCGCC GGCGC^C
BbrPI 4078 CACGTG CAC^GTG
BlpI 3751 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3751 GCTCAGC GC^T[ACGT]AGC
BsaAI 4078 CACGTG [CT]AC^GT[GA]
BsaBI 3663 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1897 TGTACA T^GTACA
BspHI 4201 TCATGA T^CATGA
BspLU11I 1874 ACATGT A^CATGT
BspMI 994 GCAGGT ACCTGC(4/8)
BsrBI 51 CCGCTC CCGCTC(-3/-3)
BsrFI 173 GCCGGC [GA]^CCGG[CT]
BsrGI 1897 TGTACA T^GTACA
BssSI 652 CTCGTG CACGAG(-5/-1)
BstEII 663 GGTCACC G^GT[ACGT]ACC
BstPI 663 GGTCACC G^GT[ACGT]ACC
BstUI 5 CGCG CG^CG
CelII 3751 GCTCAGC GC^T[ACGT]AGC
Cfr10I 173 GCCGGC [GA]^CCGG[CT]
CpoI 178 CGGACCG CG^G[AT]CCG
DraIII 3837 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 108 GACCTCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4205 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1557 AGCGCT AGC^GCT
EcoO65I 663 GGTCACC G^GT[ACGT]ACC
EcoT22I 3712 ATGCAT ATGCA^T
HindIII 1305 AAGCTT A^AGCTT
KasI 164 GGCGCC G^GCGCC
KpnI 80 GGTACC GGTAC^C
MamI 3663 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 790 CAGCTG C[AC]G^C[GT]G
MvnI 5 CGCG CG^CG
NaeI 173 GCCGGC GCC^GGC
NarI 164 GGCGCC GG^CGCC
NdeI 334 CATATG CA^TATG
NgoMIV 173 GCCGGC G^CCGGC
NsiI 3712 ATGCAT ATGCA^T
PaeR7I 26 CTCGAG C^TCGAG
PmaCI 4078 CACGTG CAC^GTG
PmlI 4078 CACGTG CAC^GTG
PshAI 2412 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3557 ATTAAT AT^TAAT
Psp1406I 3939 AACGTT AA^CGTT
PstI 3588 CTGCAG CTGCA^G
PvuII 790 CAGCTG CAG^CTG
RcaI 4201 TCATGA T^CATGA
RsrII 178 CGGACCG CG^G[AT]CCG
SexAI 3682 ACCTGGT A^CC[AT]GGT
SfoI 164 GGCGCC GGC^GCC
SgrAI 172 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1897 TGTACA T^GTACA
StuI 20 AGGCCT AGG^CCT
XbaI 2226 TCTAGA T^CTAGA
XhoI 26 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 564 GACGTC GACGT^C
1727 GACGTC
AflII 914 CTTAAG C^TTAAG
4137 CTTAAG
AocI 16 CCTCAGG CC^T[ACGT]AGG
146 CCTCAGG
Asp700I 641 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3504 GAATAGCTTC
AvaI 26 CTCGAG C^[CT]CG[GA]G
3158 CTCGGG
BalI 828 TGGCCA TGG^CCA
3112 TGGCCA
BbvCI 468 CCTCAGC CCTCAGC(-5/-2)
3021 CCTCAGC
BcgI 3010 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
796 GCACTCCTGTCG
BcnI 949 CCGGG CC^[GC]GG
957 CCCGG
BfrI 914 CTTAAG C^TTAAG
4137 CTTAAG
BsoBI 26 CTCGAG C^[CT]CG[GA]G
3158 CTCGGG
BsrDI 1398 GCAATG GCAATG(2/0)
2961 GCAATG
BstYI 260 GGATCT [GA]^GATC[CT]
726 AGATCC
Bsu36I 16 CCTCAGG CC^T[ACGT]AGG
146 CCTCAGG
DsaI 419 CCACGG C^C[GA][CT]GG
3986 CCACGG
Eco81I 16 CCTCAGG CC^T[ACGT]AGG
146 CCTCAGG
HaeII 164 GGCGCC [GA]GCGC^[CT]
1557 AGCGCT
MflI 260 GGATCT [GA]^GATC[CT]
726 AGATCC
MluNI 828 TGGCCA TGG^CCA
3112 TGGCCA
MscI 828 TGGCCA TGG^CCA
3112 TGGCCA
NciI 949 CCGGG CC^[GC]GG
957 CCCGG
XhoII 260 GGATCT [GA]^GATC[CT]
726 AGATCC
XmnI 641 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3504 GAATAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MslI MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I