##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AhdI Alw44I AlwNI Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BalI BbrPI BcgI BciVI
BglI BlnI BsaAI BsaI BsiEI
BsiWI BspDI BspHI BspMI BsrDI
BstAPI BstEII BstPI BstXI ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FseI FspI HpaI
KpnI MluI MluNI MscI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PmaCI
PmeI PmlI PshAI PvuI PvuII
RcaI RsrII SapI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 273 GACGTC GACGT^C
AccIII 209 TCCGGA T^CCGGA
AclI 84 AACGTT AA^CGTT
AflII 4065 CTTAAG C^TTAAG
AflIII 3796 ACATGT A^C[GA][CT]GT
AgeI 3914 ACCGGT A^CCGGT
AocI 1547 CCTCAGG CC^T[ACGT]AGG
ApaI 223 GGGCCC GGGCC^C
BamHI 574 GGATCC G^GATCC
BanI 280 GGCGCC G^G[CT][GA]CC
BbeI 280 GGCGCC GGCGC^C
BbsI 3978 GAAGAC GAAGAC(2/6)
BbvCI 8 GCTGAGG CCTCAGC(-5/-2)
BclI 2063 TGATCA T^GATCA
BcnI 1893 CCCGG CC^[GC]GG
BfrI 4065 CTTAAG C^TTAAG
BglII 734 AGATCT A^GATCT
BpuAI 3978 GAAGAC GAAGAC(2/6)
BseAI 209 TCCGGA T^CCGGA
BsgI 412 GTGCAG GTGCAG(16/14)
BsmI 2133 GAATGC GAATGC(1/-1)
Bsp1407I 4034 TGTACA T^GTACA
BspEI 209 TCCGGA T^CCGGA
BspLU11I 3796 ACATGT A^CATGT
BsrFI 3914 ACCGGT [GA]^CCGG[CT]
BsrGI 4034 TGTACA T^GTACA
BssHII 335 GCGCGC G^CGCGC
BssSI 409 CTCGTG CACGAG(-5/-1)
Bst1107I 477 GTATAC GTA^TAC
BstZ17I 477 GTATAC GTA^TAC
Bsu36I 1547 CCTCAGG CC^T[ACGT]AGG
Cfr10I 3914 ACCGGT [GA]^CCGG[CT]
Eco81I 1547 CCTCAGG CC^T[ACGT]AGG
EcoNI 2680 CCTATTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 558 GATATC GAT^ATC
FbaI 2063 TGATCA T^GATCA
HindIII 3853 AAGCTT A^AGCTT
KasI 280 GGCGCC G^GCGCC
KspI 701 CCGCGG CCGC^GG
MfeI 1133 CAATTG C^AATTG
MroI 209 TCCGGA T^CCGGA
MspA1I 701 CCGCGG C[AC]G^C[GT]G
MunI 1133 CAATTG C^AATTG
NarI 280 GGCGCC GG^CGCC
NciI 1893 CCCGG CC^[GC]GG
PinAI 3914 ACCGGT A^CCGGT
Psp1406I 84 AACGTT AA^CGTT
SacI 977 GAGCTC GAGCT^C
SacII 701 CCGCGG CCGC^GG
SalI 150 GTCGAC G^TCGAC
SfoI 280 GGCGCC GGC^GCC
SspBI 4034 TGTACA T^GTACA
StuI 416 AGGCCT AGG^CCT
XbaI 3864 TCTAGA T^CTAGA
XcmI 1876 CCACAACTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AseI 2213 ATTAAT AT^TAAT
3731 ATTAAT
AsnI 2213 ATTAAT AT^TAAT
3731 ATTAAT
Asp700I 732 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1281 GAACACCTTC
AspHI 297 GAGCAC G[AT]GC[AT]^C
977 GAGCTC
AspI 1120 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2164 GACAAAGTC
BlpI 294 GCTGAGC GC^T[ACGT]AGC
821 GCTTAGC
BpmI 553 CTCCAG CTGGAG(16/14)
2802 CTCCAG
Bpu1102I 294 GCTGAGC GC^T[ACGT]AGC
821 GCTTAGC
BsaWI 209 TCCGGA [AT]^CCGG[AT]
3914 ACCGGT
BsiHKAI 297 GAGCAC G[AT]GC[AT]^C
977 GAGCTC
BsmBI 893 CGTCTC CGTCTC(1/5)
1512 CGTCTC
BsrBI 27 GAGCGG CCGCTC(-3/-3)
312 GAGCGG
BstBI 1359 TTCGAA TT^CGAA
2476 TTCGAA
CelII 294 GCTGAGC GC^T[ACGT]AGC
821 GCTTAGC
DsaI 376 CCGTGG C^C[GA][CT]GG
701 CCGCGG
EarI 2505 CTCTTC CTCTTC(1/4)
396 GAAGAG
FauI 109 CCCGC CCCGC(4/6)
262 CCCGC
HaeII 44 GGCGCT [GA]GCGC^[CT]
280 GGCGCC
HincII 150 GTCGAC GT[CT]^[GA]AC
543 GTTGAC
HindII 150 GTCGAC GT[CT]^[GA]AC
543 GTTGAC
Ksp632I 2505 CTCTTC CTCTTC(1/4)
396 GAAGAG
MaeII 85 ACGT A^CGT
274 ACGT
NspI 521 GCATGT [GA]CATG^[CT]
3796 ACATGT
NspV 1359 TTCGAA TT^CGAA
2476 TTCGAA
PflFI 1120 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2164 GACAAAGTC
PflMI 482 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
925 CCAACTGTTGG
PpuMI 1543 AGGACCT [GA]G^G[AT]CC[CT]
3156 AGGACCC
PshBI 2213 ATTAAT AT^TAAT
3731 ATTAAT
PstI 807 CTGCAG CTGCA^G
3494 CTGCAG
SfuI 1359 TTCGAA TT^CGAA
2476 TTCGAA
Tsp45I 1252 GTGAC ^GT[GC]AC
2162 GTGAC
Tth111I 1120 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2164 GACAAAGTC
Van91I 482 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
925 CCAACTGTTGG
XmnI 732 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1281 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AvaI
AvaII BanII BbvI BfaI BmyI
BsaBI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII