##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AocI AscI AseI AsnI
Asp700I Asp718I AvrII BbrPI BciVI
BclI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI HindIII KpnI MamI
MfeI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 2812 CTTAAG C^TTAAG
AhdI 3002 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 172 GTGCAC G^TGCAC
Aor51HI 1506 AGCGCT AGC^GCT
ApaLI 172 GTGCAC G^TGCAC
AspEI 3002 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2090 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 116 TGCGCA TGC^GCA
BcgI 589 CGAACAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2812 CTTAAG C^TTAAG
BsiEI 65 CGGTCG CG[GA][CT]^CG
BspLU11I 2565 ACATGT A^CATGT
BsrDI 3339 CATTGC GCAATG(2/0)
BsrFI 1560 GCCGGC [GA]^CCGG[CT]
BstXI 3296 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1560 GCCGGC [GA]^CCGG[CT]
DraI 4090 TTTAAA TTT^AAA
DraIII 2830 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 879 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3002 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1506 AGCGCT AGC^GCT
EcoRV 837 GATATC GAT^ATC
FspI 116 TGCGCA TGC^GCA
HincII 2298 GTTAAC GT[CT]^[GA]AC
HindII 2298 GTTAAC GT[CT]^[GA]AC
HpaI 2298 GTTAAC GTT^AAC
KspI 12 CCGCGG CCGC^GG
MluI 1017 ACGCGT A^CGCGT
NaeI 1560 GCCGGC GCC^GGC
NgoMIV 1560 GCCGGC G^CCGGC
PflFI 2090 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1583 AGGACCT [GA]G^G[AT]CC[CT]
SacII 12 CCGCGG CCGC^GG
SfiI 1893 GGCCCAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 3552 AATATT AAT^ATT
Tth111I 2090 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 488 GGCGCC G[GA]^CG[CT]C
1190 GGCGCC
AflIII 1017 ACGCGT A^C[GA][CT]GT
2565 ACATGT
AvaI 95 CCCGGG C^[CT]CG[GA]G
1275 CCCGGG
BalI 222 TGGCCA TGG^CCA
3242 TGGCCA
BbeI 488 GGCGCC GGCGC^C
1190 GGCGCC
BbvCI 3151 CCTCAGC CCTCAGC(-5/-2)
1149 GCTGAGG
BlpI 705 GCTGAGC GC^T[ACGT]AGC
1509 GCTGAGC
Bpu1102I 705 GCTGAGC GC^T[ACGT]AGC
1509 GCTGAGC
BsaHI 488 GGCGCC G[GA]^CG[CT]C
1190 GGCGCC
BsmBI 718 GAGACG CGTCTC(1/5)
1014 GAGACG
BsoBI 95 CCCGGG C^[CT]CG[GA]G
1275 CCCGGG
BspHI 669 TCATGA T^CATGA
4265 TCATGA
BsrBI 781 GAGCGG CCGCTC(-3/-3)
964 GAGCGG
CelII 705 GCTGAGC GC^T[ACGT]AGC
1509 GCTGAGC
Hin1I 488 GGCGCC G[GA]^CG[CT]C
1190 GGCGCC
KasI 488 GGCGCC G^GCGCC
1190 GGCGCC
MluNI 222 TGGCCA TGG^CCA
3242 TGGCCA
MscI 222 TGGCCA TGG^CCA
3242 TGGCCA
NarI 488 GGCGCC GG^CGCC
1190 GGCGCC
NcoI 356 CCATGG C^CATGG
890 CCATGG
NheI 30 GCTAGC G^CTAGC
1287 GCTAGC
RcaI 669 TCATGA T^CATGA
4265 TCATGA
SbfI 492 CCTGCAGG CCTGCA^GG
1596 CCTGCAGG
SfoI 488 GGCGCC GGC^GCC
1190 GGCGCC
SmaI 95 CCCGGG CCC^GGG
1275 CCCGGG
Sse8387I 492 CCTGCAGG CCTGCA^GG
1596 CCTGCAGG
XcmI 2076 CCACTCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3344 CCATATCTGAAATGG
XmaCI 95 CCCGGG C^CCGGG
1275 CCCGGG
XmaI 95 CCCGGG C^CCGGG
1275 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BamHI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII