##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AscI        
Asp718I     AspI        BamHI       BbeI        BbvCI       
BcgI        BlpI        Bpu1102I    BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraIII      DrdI        EagI        EclXI       
Eco52I      EcoO65I     EcoRV       EcoT22I     FseI        
KasI        KpnI        KspI        MluI        NaeI        
NarI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmeI        
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SspBI       SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         2437  GTAGAC               GT^[AC][GT]AC
AccIII       3052  TCCGGA               T^CCGGA
AcyI         3208  GACGCC               G[GA]^CG[CT]C
AocI         1836  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        53  AGCGCT               AGC^GCT
ApaI         1336  GGGCCC               GGGCC^C
AvaI          342  CTCGAG               C^[CT]CG[GA]G
AviII        3396  TGCGCA               TGC^GCA
BglI         1456  GCCCTTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1996  AGATCT               A^GATCT
BsaHI        3208  GACGCC               G[GA]^CG[CT]C
BsaWI        3052  TCCGGA               [AT]^CCGG[AT]
BseAI        3052  TCCGGA               T^CCGGA
BsoBI         342  CTCGAG               C^[CT]CG[GA]G
BspEI        3052  TCCGGA               T^CCGGA
BsrBI        3540  CCGCTC               CCGCTC(-3/-3)
BssSI        3353  CTCGTG               CACGAG(-5/-1)
Bsu36I       1836  CCTGAGG              CC^T[ACGT]AGG
DraII        3308  AGGTCCC              [GA]G^G[ACGT]CC[CT]
Eco47III       53  AGCGCT               AGC^GCT
Eco81I       1836  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     3308  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1902  GAATTC               G^AATTC
FspI         3396  TGCGCA               TGC^GCA
HaeII          53  AGCGCT               [GA]GCGC^[CT]
HgaI         3208  GACGC                GACGC(5/10)
Hin1I        3208  GACGCC               G[GA]^CG[CT]C
HindIII       431  AAGCTT               A^AGCTT
HpaI          762  GTTAAC               GTT^AAC
MfeI         2866  CAATTG               C^AATTG
MroI         3052  TCCGGA               T^CCGGA
MunI         2866  CAATTG               C^AATTG
NdeI         2191  CATATG               CA^TATG
NheI         4042  GCTAGC               G^CTAGC
PaeR7I        342  CTCGAG               C^TCGAG
PpuMI        3308  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        3432  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         921  CAGCTG               CAG^CTG
SacI         2090  GAGCTC               GAGCT^C
SapI         4128  GAAGAGC              GCTCTTC(1/4)
SexAI        3344  ACCTGGT              A^CC[AT]GGT
SpeI         2981  ACTAGT               A^CTAGT
SphI         1675  GCATGC               GCATG^C
StuI         2600  AGGCCT               AGG^CCT
XbaI         3158  TCTAGA               T^CTAGA
XhoI          342  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AhdI          191  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1701  GACAGTCGGTC         
Alw44I       2640  GTGCAC               G^TGCAC
             4227  GTGCAC              
ApaLI        2640  GTGCAC               G^TGCAC
             4227  GTGCAC              
Asp700I       863  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1197  GAAAATATTC          
AspEI         191  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1701  GACAGTCGGTC         
AvrII        2532  CCTAGG               C^CTAGG
             3570  CCTAGG              
BalI         1613  TGGCCA               TGG^CCA
             2346  TGGCCA              
BbrPI        1069  CACGTG               CAC^GTG
             1078  CACGTG              
BbsI         1584  GAAGAC               GAAGAC(2/6)
             3439  GTCTTC              
BciVI         320  GTATCC               GTATCC(6/5)
             3138  GTATCC              
BlnI         2532  CCTAGG               C^CTAGG
             3570  CCTAGG              
BpuAI        1584  GAAGAC               GAAGAC(2/6)
             3439  GTCTTC              
BsaAI        1069  CACGTG               [CT]AC^GT[GA]
             1078  CACGTG              
BsaBI        1991  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2685  GATTCTCATC          
BsaI         2184  GAGACC               GGTCTC(1/5)
             2110  GGTCTC              
BsrFI        3243  ACCGGC               [GA]^CCGG[CT]
             3591  GCCGGT              
BssHII         71  GCGCGC               G^CGCGC
               73  GCGCGC              
BstXI         892  CCAGCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1066  CCACACGTGTGG        
Cfr10I       3243  ACCGGC               [GA]^CCGG[CT]
             3591  GCCGGT              
Eam1105I      191  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1701  GACAGTCGGTC         
EcoNI        2791  CCTCCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3545  CCTGTAGAAGG         
FauI           13  CCCGC                CCCGC(4/6)
             1339  CCCGC               
MamI         1991  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2685  GATTCTCATC          
MluNI        1613  TGGCCA               TGG^CCA
             2346  TGGCCA              
MscI         1613  TGGCCA               TGG^CCA
             2346  TGGCCA              
NcoI         1977  CCATGG               C^CATGG
             3733  CCATGG              
PmaCI        1069  CACGTG               CAC^GTG
             1078  CACGTG              
PmlI         1069  CACGTG               CAC^GTG
             1078  CACGTG              
XmnI          863  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1197  GAAAATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflII       
AflIII      AluI        AlwI        AlwNI       ApoI        
AseI        AsnI        AspHI       AvaII       BanI        
BanII       BbvI        BclI        BcnI        BfaI        
BfrI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DsaI        EaeI        EarI        EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PshBI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII