################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AclI AgeI AscI Asp718I AspI BamHI BbeI BbvCI BcgI BlpI Bpu1102I BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI KasI KpnI KspI MluI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2437 GTAGAC GT^[AC][GT]AC AccIII 3052 TCCGGA T^CCGGA AcyI 3208 GACGCC G[GA]^CG[CT]C AocI 1836 CCTGAGG CC^T[ACGT]AGG Aor51HI 53 AGCGCT AGC^GCT ApaI 1336 GGGCCC GGGCC^C AvaI 342 CTCGAG C^[CT]CG[GA]G AviII 3396 TGCGCA TGC^GCA BglI 1456 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1996 AGATCT A^GATCT BsaHI 3208 GACGCC G[GA]^CG[CT]C BsaWI 3052 TCCGGA [AT]^CCGG[AT] BseAI 3052 TCCGGA T^CCGGA BsoBI 342 CTCGAG C^[CT]CG[GA]G BspEI 3052 TCCGGA T^CCGGA BsrBI 3540 CCGCTC CCGCTC(-3/-3) BssSI 3353 CTCGTG CACGAG(-5/-1) Bsu36I 1836 CCTGAGG CC^T[ACGT]AGG DraII 3308 AGGTCCC [GA]G^G[ACGT]CC[CT] Eco47III 53 AGCGCT AGC^GCT Eco81I 1836 CCTGAGG CC^T[ACGT]AGG EcoO109I 3308 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 1902 GAATTC G^AATTC FspI 3396 TGCGCA TGC^GCA HaeII 53 AGCGCT [GA]GCGC^[CT] HgaI 3208 GACGC GACGC(5/10) Hin1I 3208 GACGCC G[GA]^CG[CT]C HindIII 431 AAGCTT A^AGCTT HpaI 762 GTTAAC GTT^AAC MfeI 2866 CAATTG C^AATTG MroI 3052 TCCGGA T^CCGGA MunI 2866 CAATTG C^AATTG NdeI 2191 CATATG CA^TATG NheI 4042 GCTAGC G^CTAGC PaeR7I 342 CTCGAG C^TCGAG PpuMI 3308 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 3432 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 921 CAGCTG CAG^CTG SacI 2090 GAGCTC GAGCT^C SapI 4128 GAAGAGC GCTCTTC(1/4) SexAI 3344 ACCTGGT A^CC[AT]GGT SpeI 2981 ACTAGT A^CTAGT SphI 1675 GCATGC GCATG^C StuI 2600 AGGCCT AGG^CCT XbaI 3158 TCTAGA T^CTAGA XhoI 342 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AhdI 191 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1701 GACAGTCGGTC Alw44I 2640 GTGCAC G^TGCAC 4227 GTGCAC ApaLI 2640 GTGCAC G^TGCAC 4227 GTGCAC Asp700I 863 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1197 GAAAATATTC AspEI 191 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1701 GACAGTCGGTC AvrII 2532 CCTAGG C^CTAGG 3570 CCTAGG BalI 1613 TGGCCA TGG^CCA 2346 TGGCCA BbrPI 1069 CACGTG CAC^GTG 1078 CACGTG BbsI 1584 GAAGAC GAAGAC(2/6) 3439 GTCTTC BciVI 320 GTATCC GTATCC(6/5) 3138 GTATCC BlnI 2532 CCTAGG C^CTAGG 3570 CCTAGG BpuAI 1584 GAAGAC GAAGAC(2/6) 3439 GTCTTC BsaAI 1069 CACGTG [CT]AC^GT[GA] 1078 CACGTG BsaBI 1991 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2685 GATTCTCATC BsaI 2184 GAGACC GGTCTC(1/5) 2110 GGTCTC BsrFI 3243 ACCGGC [GA]^CCGG[CT] 3591 GCCGGT BssHII 71 GCGCGC G^CGCGC 73 GCGCGC BstXI 892 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1066 CCACACGTGTGG Cfr10I 3243 ACCGGC [GA]^CCGG[CT] 3591 GCCGGT Eam1105I 191 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1701 GACAGTCGGTC EcoNI 2791 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3545 CCTGTAGAAGG FauI 13 CCCGC CCCGC(4/6) 1339 CCCGC MamI 1991 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2685 GATTCTCATC MluNI 1613 TGGCCA TGG^CCA 2346 TGGCCA MscI 1613 TGGCCA TGG^CCA 2346 TGGCCA NcoI 1977 CCATGG C^CATGG 3733 CCATGG PmaCI 1069 CACGTG CAC^GTG 1078 CACGTG PmlI 1069 CACGTG CAC^GTG 1078 CACGTG XmnI 863 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1197 GAAAATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanI BanII BbvI BclI BcnI BfaI BfrI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII