##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AflII Aor51HI
AscI Asp718I AspI AviII BcgI
BfrI BsiEI BsiWI Bsp1407I BspDI
BsrGI BssHII BstBI BstEII BstPI
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KpnI KspI MluI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SexAI
SfuI SgrAI SnaBI SpeI SspBI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 3929 CGCG CG^CG
AccIII 59 TCCGGA T^CCGGA
AcyI 4397 GGCGCC G[GA]^CG[CT]C
AflIII 3618 ACATGT A^C[GA][CT]GT
AgeI 476 ACCGGT A^CCGGT
ApaI 3709 GGGCCC GGGCC^C
AseI 2901 ATTAAT AT^TAAT
AsnI 2901 ATTAAT AT^TAAT
AvrII 3517 CCTAGG C^CTAGG
BbeI 4397 GGCGCC GGCGC^C
BbsI 3645 GTCTTC GAAGAC(2/6)
BbvCI 2246 GCTGAGG CCTCAGC(-5/-2)
BclI 1393 TGATCA T^GATCA
BlnI 3517 CCTAGG C^CTAGG
BlpI 3141 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3141 GCTCAGC GC^T[ACGT]AGC
BpuAI 3645 GTCTTC GAAGAC(2/6)
BsaHI 4397 GGCGCC G[GA]^CG[CT]C
BseAI 59 TCCGGA T^CCGGA
BspEI 59 TCCGGA T^CCGGA
BspHI 553 TCATGA T^CATGA
BspLU11I 3618 ACATGT A^CATGT
Bst1107I 4637 GTATAC GTA^TAC
BstUI 3929 CGCG CG^CG
BstZ17I 4637 GTATAC GTA^TAC
CelII 3141 GCTCAGC GC^T[ACGT]AGC
DraI 3958 TTTAAA TTT^AAA
FauI 3930 GCGGG CCCGC(4/6)
FbaI 1393 TGATCA T^GATCA
HaeII 4397 GGCGCC [GA]GCGC^[CT]
Hin1I 4397 GGCGCC G[GA]^CG[CT]C
KasI 4397 GGCGCC G^GCGCC
MfeI 1367 CAATTG C^AATTG
MroI 59 TCCGGA T^CCGGA
MunI 1367 CAATTG C^AATTG
MvnI 3929 CGCG CG^CG
NaeI 2121 GCCGGC GCC^GGC
NarI 4397 GGCGCC GG^CGCC
NgoMIV 2121 GCCGGC G^CCGGC
PaeR7I 2414 CTCGAG C^TCGAG
PinAI 476 ACCGGT A^CCGGT
PshBI 2901 ATTAAT AT^TAAT
RcaI 553 TCATGA T^CATGA
SapI 2591 GAAGAGC GCTCTTC(1/4)
SbfI 2015 CCTGCAGG CCTGCA^GG
ScaI 2253 AGTACT AGT^ACT
SfiI 1510 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4397 GGCGCC GGC^GCC
SphI 4272 GCATGC GCATG^C
Sse8387I 2015 CCTGCAGG CCTGCA^GG
SspI 3376 AATATT AAT^ATT
StuI 4346 AGGCCT AGG^CCT
XhoI 2414 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 2909 GACTCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4099 GACTCGAAGTC
Alw44I 3022 GTGCAC G^TGCAC
4013 GTGCAC
ApaLI 3022 GTGCAC G^TGCAC
4013 GTGCAC
Asp700I 200 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2533 GAAACGGTTC
AspEI 2909 GACTCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4099 GACTCGAAGTC
BamHI 28 GGATCC G^GATCC
2767 GGATCC
BbrPI 1571 CACGTG CAC^GTG
1842 CACGTG
BciVI 2233 GGATAC GTATCC(6/5)
1279 GTATCC
BglII 1816 AGATCT A^GATCT
4752 AGATCT
BsaAI 1571 CACGTG [CT]AC^GT[GA]
1842 CACGTG
BsaBI 2297 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4747 GATAAAGATC
BsaI 473 GAGACC GGTCTC(1/5)
3408 GGTCTC
BsaWI 59 TCCGGA [AT]^CCGG[AT]
476 ACCGGT
BsmBI 3235 CGTCTC CGTCTC(1/5)
93 GAGACG
BsmI 2340 GCATTC GAATGC(1/-1)
3215 GCATTC
Eam1105I 2909 GACTCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4099 GACTCGAAGTC
EcoRI 2573 GAATTC G^AATTC
2993 GAATTC
HindIII 3185 AAGCTT A^AGCTT
4728 AAGCTT
MamI 2297 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4747 GATAAAGATC
NspI 3618 ACATGT [GA]CATG^[CT]
4272 GCATGC
PmaCI 1571 CACGTG CAC^GTG
1842 CACGTG
PmlI 1571 CACGTG CAC^GTG
1842 CACGTG
PpuMI 2409 AGGTCCT [GA]G^G[AT]CC[CT]
3834 GGGTCCC
SacI 683 GAGCTC GAGCT^C
2166 GAGCTC
SmaI 4084 CCCGGG CCC^GGG
4441 CCCGGG
XmaCI 4084 CCCGGG C^CCGGG
4441 CCCGGG
XmaI 4084 CCCGGG C^CCGGG
4441 CCCGGG
XmnI 200 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2533 GAAACGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII