##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BbrPI BglII BlnI
BseAI BsiWI BspDI BspEI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HpaI KasI KpnI KspI
MroI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 2894 CTTAAG C^TTAAG
AhdI 2680 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4224 GTGCAC G^TGCAC
Aor51HI 1757 AGCGCT AGC^GCT
ApaLI 4224 GTGCAC G^TGCAC
AspEI 2680 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2967 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 807 GGATCC G^GATCC
BbsI 3162 GAAGAC GAAGAC(2/6)
BciVI 2808 GTATCC GTATCC(6/5)
BclI 279 TGATCA T^GATCA
BfrI 2894 CTTAAG C^TTAAG
BglI 2014 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1921 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1921 GCTGAGC GC^T[ACGT]AGC
BpuAI 3162 GAAGAC GAAGAC(2/6)
BsaBI 713 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2768 GAGACC GGTCTC(1/5)
BsaWI 2225 TCCGGT [AT]^CCGG[AT]
BspHI 188 TCATGA T^CATGA
BspLU11I 3354 ACATGT A^CATGT
BsrBI 2200 GAGCGG CCGCTC(-3/-3)
BsrDI 157 GCAATG GCAATG(2/0)
CelII 1921 GCTGAGC GC^T[ACGT]AGC
DraI 306 TTTAAA TTT^AAA
EagI 3406 CGGCCG C^GGCCG
Eam1105I 2680 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3406 CGGCCG C^GGCCG
Eco47III 1757 AGCGCT AGC^GCT
Eco52I 3406 CGGCCG C^GGCCG
FbaI 279 TGATCA T^GATCA
MamI 713 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 295 CAATTG C^AATTG
MluI 2255 ACGCGT A^CGCGT
MunI 295 CAATTG C^AATTG
NspI 3354 ACATGT [GA]CATG^[CT]
PflFI 2967 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 188 TCATGA T^CATGA
ScaI 1504 AGTACT AGT^ACT
SmaI 927 CCCGGG CCC^GGG
SnaBI 3918 TACGTA TAC^GTA
Tth111I 2967 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 927 CCCGGG C^CCGGG
XmaI 927 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 2255 ACGCGT A^C[GA][CT]GT
3354 ACATGT
AocI 2718 CCTGAGG CC^T[ACGT]AGG
4020 CCTTAGG
ApaI 1719 GGGCCC GGGCC^C
1729 GGGCCC
Asp700I 94 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
324 GAATGGGTTC
BcgI 3251 CGACCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3829 CGATCTCCCTGC
BsaAI 1158 CACGTA [CT]AC^GT[GA]
3918 TACGTA
BsiEI 3251 CGACCG CG[GA][CT]^CG
3406 CGGCCG
BsmI 2415 GAATGC GAATGC(1/-1)
1611 GCATTC
Bsp1407I 4073 TGTACA T^GTACA
4124 TGTACA
BsrGI 4073 TGTACA T^GTACA
4124 TGTACA
BstXI 3465 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4103 CCATCACCTTGG
Bsu36I 2718 CCTGAGG CC^T[ACGT]AGG
4020 CCTTAGG
DraIII 2377 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
2976 CACAATGTG
Eco81I 2718 CCTGAGG CC^T[ACGT]AGG
4020 CCTTAGG
EcoRI 756 GAATTC G^AATTC
1296 GAATTC
HindIII 97 AAGCTT A^AGCTT
642 AAGCTT
MslI 474 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3901 CACTGAGGTG
PflMI 952 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3025 CCATTAACTGG
PstI 2546 CTGCAG CTGCA^G
2927 CTGCAG
SacI 1885 GAGCTC GAGCT^C
1981 GAGCTC
SmlI 2894 CTTAAG C^T[CT][GA]AG
3880 CTCAAG
SspBI 4073 TGTACA T^GTACA
4124 TGTACA
Van91I 952 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3025 CCATTAACTGG
XmnI 94 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
324 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PvuII RsaI SalI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII