##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BciVI BfrI BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FseI FspI
HpaI KpnI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PflFI PmeI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2800 GTAGAC GT^[AC][GT]AC
AgeI 2204 ACCGGT A^CCGGT
Alw44I 2994 GTGCAC G^TGCAC
ApaI 1572 GGGCCC GGGCC^C
ApaLI 2994 GTGCAC G^TGCAC
BbeI 1811 GGCGCC GGCGC^C
BbrPI 448 CACGTG CAC^GTG
BbvCI 2733 CCTCAGC CCTCAGC(-5/-2)
BglI 2299 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 18 AGATCT A^GATCT
BsaAI 448 CACGTG [CT]AC^GT[GA]
BsmI 2576 GCATTC GAATGC(1/-1)
BspLU11I 113 ACATGT A^CATGT
BsrDI 2673 GCAATG GCAATG(2/0)
BstAPI 2186 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1081 GGTGACC G^GT[ACGT]ACC
BstPI 1081 GGTGACC G^GT[ACGT]ACC
DraIII 952 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1081 GGTGACC G^GT[ACGT]ACC
HaeII 1811 GGCGCC [GA]GCGC^[CT]
HincII 2946 GTCAAC GT[CT]^[GA]AC
HindII 2946 GTCAAC GT[CT]^[GA]AC
KasI 1811 GGCGCC G^GCGCC
KspI 1335 CCGCGG CCGC^GG
NarI 1811 GGCGCC GG^CGCC
NcoI 699 CCATGG C^CATGG
PaeR7I 1607 CTCGAG C^TCGAG
PinAI 2204 ACCGGT A^CCGGT
PmaCI 448 CACGTG CAC^GTG
PmlI 448 CACGTG CAC^GTG
PshAI 7 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1999 CTGCAG CTGCA^G
PvuII 1835 CAGCTG CAG^CTG
SacI 3231 GAGCTC GAGCT^C
SacII 1335 CCGCGG CCGC^GG
SfoI 1811 GGCGCC GGC^GCC
SspI 2144 AATATT AAT^ATT
StuI 2344 AGGCCT AGG^CCT
XhoI 1607 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AseI 594 ATTAAT AT^TAAT
2197 ATTAAT
AsnI 594 ATTAAT AT^TAAT
2197 ATTAAT
BcgI 2722 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2255 GCAGAAGCATCG
BclI 1686 TGATCA T^GATCA
3516 TGATCA
BfaI 3444 CTAG C^TAG
3578 CTAG
BsgI 3615 CTGCAC GTGCAG(16/14)
1171 GTGCAG
BsmBI 1744 CGTCTC CGTCTC(1/5)
2637 GAGACG
BssSI 2951 CACGAG CACGAG(-5/-1)
3871 CTCGTG
EcoRI 163 GAATTC G^AATTC
278 GAATTC
EcoRV 1543 GATATC GAT^ATC
3801 GATATC
EcoT22I 2193 ATGCAT ATGCA^T
2448 ATGCAT
FauI 9 CCCGC CCCGC(4/6)
1419 GCGGG
FbaI 1686 TGATCA T^GATCA
3516 TGATCA
HindIII 1756 AAGCTT A^AGCTT
3155 AAGCTT
MaeI 3444 CTAG C^TAG
3578 CTAG
NaeI 303 GCCGGC GCC^GGC
3058 GCCGGC
NgoMIV 303 GCCGGC G^CCGGC
3058 GCCGGC
NsiI 2193 ATGCAT ATGCA^T
2448 ATGCAT
PflMI 483 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3397 CCAGACTGTGG
PpuMI 5 GGGACCC [GA]G^G[AT]CC[CT]
1483 GGGACCC
PshBI 594 ATTAAT AT^TAAT
2197 ATTAAT
SapI 106 GAAGAGC GCTCTTC(1/4)
2181 GAAGAGC
SfcI 73 CTGTAG C^T[GA][CT]AG
1999 CTGCAG
SmaI 1575 CCCGGG CCC^GGG
2710 CCCGGG
Van91I 483 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3397 CCAGACTGTGG
XcmI 3657 CCAAACCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3842 CCACTTAATGTGTGG
XmaCI 1575 CCCGGG C^CCGGG
2710 CCCGGG
XmaI 1575 CCCGGG C^CCGGG
2710 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI