##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BbeI BbrPI
BcgI BclI BlnI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HaeII HincII HindII HpaI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 956 GACGTC GACGT^C
AcsI 3984 GAATTC [GA]^AATT[CT]
AflII 143 CTTAAG C^TTAAG
ApoI 3984 GAATTC [GA]^AATT[CT]
AvaI 3216 CCCGGG C^[CT]CG[GA]G
AviII 333 TGCGCA TGC^GCA
BamHI 668 GGATCC G^GATCC
BanI 856 GGCACC G^G[CT][GA]CC
BfrI 143 CTTAAG C^TTAAG
BsaAI 584 TACGTG [CT]AC^GT[GA]
BsaI 1218 GAGACC GGTCTC(1/5)
BsaWI 2062 TCCGGT [AT]^CCGG[AT]
BsoBI 3216 CCCGGG C^[CT]CG[GA]G
BsrBI 933 CCGCTC CCGCTC(-3/-3)
BsrDI 649 CATTGC GCAATG(2/0)
BssSI 909 CACGAG CACGAG(-5/-1)
DraIII 3171 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1696 GACAAGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3984 GAATTC G^AATTC
FspI 333 TGCGCA TGC^GCA
HgaI 399 GCGTC GACGC(5/10)
MfeI 3712 CAATTG C^AATTG
MunI 3712 CAATTG C^AATTG
NdeI 2313 CATATG CA^TATG
PflMI 2426 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 3216 CCCGGG CCC^GGG
SpeI 1011 ACTAGT A^CTAGT
SphI 2976 GCATGC GCATG^C
SspI 4006 AATATT AAT^ATT
TaqI 1619 TCGA T^CGA
TthHB8I 1619 TCGA T^CGA
Van91I 2426 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1192 TCTAGA T^CTAGA
XmaCI 3216 CCCGGG C^CCGGG
XmaI 3216 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 928 GTCTAC GT^[AC][GT]AC
3235 GTAGAC
AcyI 398 GGCGTC G[GA]^CG[CT]C
956 GACGTC
AflIII 585 ACGTGT A^C[GA][CT]GT
2918 ACGTGT
AhdI 890 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2927 GACTCCATGTC
Alw44I 1315 GTGCAC G^TGCAC
3697 GTGCAC
ApaI 174 GGGCCC GGGCC^C
826 GGGCCC
ApaLI 1315 GTGCAC G^TGCAC
3697 GTGCAC
AspEI 890 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2927 GACTCCATGTC
BalI 180 TGGCCA TGG^CCA
3904 TGGCCA
BglII 1646 AGATCT A^GATCT
3309 AGATCT
BsaHI 398 GGCGTC G[GA]^CG[CT]C
956 GACGTC
BsmBI 3006 CGTCTC CGTCTC(1/5)
3119 CGTCTC
BsmI 1414 GAATGC GAATGC(1/-1)
2876 GAATGC
Bsp1407I 2886 TGTACA T^GTACA
4020 TGTACA
BsrGI 2886 TGTACA T^GTACA
4020 TGTACA
Eam1105I 890 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2927 GACTCCATGTC
Hin1I 398 GGCGTC G[GA]^CG[CT]C
956 GACGTC
HindIII 140 AAGCTT A^AGCTT
2265 AAGCTT
MluNI 180 TGGCCA TGG^CCA
3904 TGGCCA
MscI 180 TGGCCA TGG^CCA
3904 TGGCCA
PstI 3015 CTGCAG CTGCA^G
3373 CTGCAG
SacI 2956 GAGCTC GAGCT^C
3412 GAGCTC
SspBI 2886 TGTACA T^GTACA
4020 TGTACA
TfiI 2230 GATTC G^A[AT]TC
3959 GATTC
Tsp509I 3713 AATT ^AATT
3985 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI Tru9I
TseI Tsp45I TspRI XcmI XhoII