##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII BbeI BbrPI BciVI
BclI BfrI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
Bst1107I BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FbaI FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 278 CGCG CG^CG
AflIII 1176 ACATGT A^C[GA][CT]GT
Alw44I 281 GTGCAC G^TGCAC
ApaLI 281 GTGCAC G^TGCAC
AvrII 2809 CCTAGG C^CTAGG
BbsI 1559 GAAGAC GAAGAC(2/6)
BbvCI 2820 CCTCAGC CCTCAGC(-5/-2)
BglI 1320 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2809 CCTAGG C^CTAGG
BpuAI 1559 GAAGAC GAAGAC(2/6)
BsaWI 1494 ACCGGA [AT]^CCGG[AT]
BsmBI 1276 GAGACG CGTCTC(1/5)
BsmI 183 GCATTC GAATGC(1/-1)
BspHI 26 TCATGA T^CATGA
BspLU11I 1176 ACATGT A^CATGT
BsrDI 2744 GCAATG GCAATG(2/0)
BstAPI 495 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 99 TTCGAA TT^CGAA
BstUI 278 CGCG CG^CG
BstXI 2137 CCAAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 115 TTTAAA TTT^AAA
DraIII 3763 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 2246 ATGCAT ATGCA^T
MslI 166 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 278 CGCG CG^CG
NdeI 3909 CATATG CA^TATG
NsiI 2246 ATGCAT ATGCA^T
NspV 99 TTCGAA TT^CGAA
PflMI 574 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 26 TCATGA T^CATGA
ScaI 1629 AGTACT AGT^ACT
SfuI 99 TTCGAA TT^CGAA
StuI 2812 AGGCCT AGG^CCT
Van91I 574 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1531 GTCTAC GT^[AC][GT]AC
2090 GTAGAC
AcsI 2577 AAATTT [GA]^AATT[CT]
3964 AAATTC
ApaI 1376 GGGCCC GGGCC^C
2632 GGGCCC
ApoI 2577 AAATTT [GA]^AATT[CT]
3964 AAATTC
Asp700I 508 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3933 GAAAGCTTTC
AspI 2441 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3783 GACTCGGTC
BamHI 1025 GGATCC G^GATCC
2460 GGATCC
BcgI 772 CGACTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1049 CGAGTCATCTGC
BglII 2028 AGATCT A^GATCT
2648 AGATCT
BsaI 2 GAGACC GGTCTC(1/5)
3788 GGTCTC
BsiEI 1772 CGGTCG CG[GA][CT]^CG
1877 CGGTCG
BstEII 532 GGTGACC G^GT[ACGT]ACC
1038 GGTCACC
BstPI 532 GGTGACC G^GT[ACGT]ACC
1038 GGTCACC
EcoO65I 532 GGTGACC G^GT[ACGT]ACC
1038 GGTCACC
HaeII 2109 AGCGCC [GA]GCGC^[CT]
2687 AGCGCC
HgaI 4140 GACGC GACGC(5/10)
717 GCGTC
HindIII 3490 AAGCTT A^AGCTT
3935 AAGCTT
NaeI 1320 GCCGGC GCC^GGC
1740 GCCGGC
NgoMIV 1320 GCCGGC G^CCGGC
1740 GCCGGC
PflFI 2441 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3783 GACTCGGTC
SmaI 966 CCCGGG CCC^GGG
1291 CCCGGG
SphI 1713 GCATGC GCATG^C
2013 GCATGC
Tth111I 2441 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3783 GACTCGGTC
XmaCI 966 CCCGGG C^CCGGG
1291 CCCGGG
XmaI 966 CCCGGG C^CCGGG
1291 CCCGGG
XmnI 508 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3933 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII