################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## Acc65I AccI AclI AflII AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII BamHI BcgI BciVI BclI BfrI BglII BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI KpnI KspI MamI MluI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 3365 TCCGGA T^CCGGA AflIII 3613 ACACGT A^C[GA][CT]GT Aor51HI 1299 AGCGCT AGC^GCT Asp700I 1349 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 134 CCTAGG C^CTAGG BbrPI 803 CACGTG CAC^GTG BlnI 134 CCTAGG C^CTAGG BsaI 1652 GAGACC GGTCTC(1/5) BseAI 3365 TCCGGA T^CCGGA BsiEI 580 CGACCG CG[GA][CT]^CG BsmBI 1037 GAGACG CGTCTC(1/5) BsmI 3606 GAATGC GAATGC(1/-1) BspEI 3365 TCCGGA T^CCGGA BsrDI 1333 CATTGC GCAATG(2/0) DrdI 415 GACACGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 460 CCGTGG C^C[GA][CT]GG Eco47III 1299 AGCGCT AGC^GCT HincII 229 GTTAAC GT[CT]^[GA]AC HindII 229 GTTAAC GT[CT]^[GA]AC HindIII 113 AAGCTT A^AGCTT HpaI 229 GTTAAC GTT^AAC MfeI 3890 CAATTG C^AATTG MroI 3365 TCCGGA T^CCGGA MslI 1570 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 3890 CAATTG C^AATTG NheI 346 GCTAGC G^CTAGC NspI 3599 ACATGC [GA]CATG^[CT] PflMI 3555 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 803 CACGTG CAC^GTG PmlI 803 CACGTG CAC^GTG SapI 3815 GAAGAGC GCTCTTC(1/4) Van91I 3555 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1349 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 421 GACGTC GACGT^C 1066 GACGTC AhdI 1061 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1157 GACCGACTGTC AspEI 1061 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1157 GACCGACTGTC BbsI 3035 GAAGAC GAAGAC(2/6) 3320 GAAGAC BpuAI 3035 GAAGAC GAAGAC(2/6) 3320 GAAGAC BsaAI 803 CACGTG [CT]AC^GT[GA] 3614 CACGTA BsaWI 3136 ACCGGA [AT]^CCGG[AT] 3365 TCCGGA BsgI 71 CTGCAC GTGCAG(16/14) 1112 GTGCAG BsrBI 679 CCGCTC CCGCTC(-3/-3) 3818 GAGCGG BssSI 200 CACGAG CACGAG(-5/-1) 409 CTCGTG BstAPI 925 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3663 GCATTTATTGC BstYI 996 AGATCC [GA]^GATC[CT] 2721 AGATCC Eam1105I 1061 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1157 GACCGACTGTC MflI 996 AGATCC [GA]^GATC[CT] 2721 AGATCC MseI 230 TTAA T^TAA 3744 TTAA PaeR7I 1 CTCGAG C^TCGAG 788 CTCGAG PpuMI 1422 AGGACCT [GA]G^G[AT]CC[CT] 2606 AGGTCCC TfiI 1084 GATTC G^A[AT]TC 2942 GAATC Tru9I 230 TTAA T^TAA 3744 TTAA XhoI 1 CTCGAG C^TCGAG 788 CTCGAG XhoII 996 AGATCC [GA]^GATC[CT] 2721 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI