##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AviII       BamHI       
BcgI        BciVI       BclI        BfrI        BglII       
BsaBI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
EagI        EclXI       Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
KpnI        KspI        MamI        MluI        NaeI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccIII       3365  TCCGGA               T^CCGGA
AflIII       3613  ACACGT               A^C[GA][CT]GT
Aor51HI      1299  AGCGCT               AGC^GCT
Asp700I      1349  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         134  CCTAGG               C^CTAGG
BbrPI         803  CACGTG               CAC^GTG
BlnI          134  CCTAGG               C^CTAGG
BsaI         1652  GAGACC               GGTCTC(1/5)
BseAI        3365  TCCGGA               T^CCGGA
BsiEI         580  CGACCG               CG[GA][CT]^CG
BsmBI        1037  GAGACG               CGTCTC(1/5)
BsmI         3606  GAATGC               GAATGC(1/-1)
BspEI        3365  TCCGGA               T^CCGGA
BsrDI        1333  CATTGC               GCAATG(2/0)
DrdI          415  GACACGGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          460  CCGTGG               C^C[GA][CT]GG
Eco47III     1299  AGCGCT               AGC^GCT
HincII        229  GTTAAC               GT[CT]^[GA]AC
HindII        229  GTTAAC               GT[CT]^[GA]AC
HindIII       113  AAGCTT               A^AGCTT
HpaI          229  GTTAAC               GTT^AAC
MfeI         3890  CAATTG               C^AATTG
MroI         3365  TCCGGA               T^CCGGA
MslI         1570  CATAAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         3890  CAATTG               C^AATTG
NheI          346  GCTAGC               G^CTAGC
NspI         3599  ACATGC               [GA]CATG^[CT]
PflMI        3555  CCAGGCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         803  CACGTG               CAC^GTG
PmlI          803  CACGTG               CAC^GTG
SapI         3815  GAAGAGC              GCTCTTC(1/4)
Van91I       3555  CCAGGCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1349  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AatII         421  GACGTC               GACGT^C
             1066  GACGTC              
AhdI         1061  GACGTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1157  GACCGACTGTC         
AspEI        1061  GACGTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1157  GACCGACTGTC         
BbsI         3035  GAAGAC               GAAGAC(2/6)
             3320  GAAGAC              
BpuAI        3035  GAAGAC               GAAGAC(2/6)
             3320  GAAGAC              
BsaAI         803  CACGTG               [CT]AC^GT[GA]
             3614  CACGTA              
BsaWI        3136  ACCGGA               [AT]^CCGG[AT]
             3365  TCCGGA              
BsgI           71  CTGCAC               GTGCAG(16/14)
             1112  GTGCAG              
BsrBI         679  CCGCTC               CCGCTC(-3/-3)
             3818  GAGCGG              
BssSI         200  CACGAG               CACGAG(-5/-1)
              409  CTCGTG              
BstAPI        925  GCAGGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3663  GCATTTATTGC         
BstYI         996  AGATCC               [GA]^GATC[CT]
             2721  AGATCC              
Eam1105I     1061  GACGTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1157  GACCGACTGTC         
MflI          996  AGATCC               [GA]^GATC[CT]
             2721  AGATCC              
MseI          230  TTAA                 T^TAA
             3744  TTAA                
PaeR7I          1  CTCGAG               C^TCGAG
              788  CTCGAG              
PpuMI        1422  AGGACCT              [GA]G^G[AT]CC[CT]
             2606  AGGTCCC             
TfiI         1084  GATTC                G^A[AT]TC
             2942  GAATC               
Tru9I         230  TTAA                 T^TAA
             3744  TTAA                
XhoI            1  CTCGAG               C^TCGAG
              788  CTCGAG              
XhoII         996  AGATCC               [GA]^GATC[CT]
             2721  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       BstXI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI