##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AscI AseI AsnI Asp718I
AspI BbeI BciVI BfrI BlpI
Bpu1102I BsaHI BsaI BsiWI BspDI
BsrDI BssSI Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI EagI
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT22I FseI Hin1I HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SbfI
ScaI SexAI SfiI SfoI SgrAI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AocI 3186 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2838 AGCGCT AGC^GCT
ApaI 736 GGGCCC GGGCC^C
AviII 630 TGCGCA TGC^GCA
AvrII 45 CCTAGG C^CTAGG
BalI 1256 TGGCCA TGG^CCA
BbrPI 2284 CACGTG CAC^GTG
BbsI 1065 GTCTTC GAAGAC(2/6)
BcgI 593 GCACATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 346 AGATCT A^GATCT
BlnI 45 CCTAGG C^CTAGG
BpuAI 1065 GTCTTC GAAGAC(2/6)
BsaBI 358 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 783 CGACCG CG[GA][CT]^CG
BsmBI 1632 CGTCTC CGTCTC(1/5)
BsmI 2930 GAATGC GAATGC(1/-1)
BsrBI 2201 CCGCTC CCGCTC(-3/-3)
BssHII 1980 GCGCGC G^CGCGC
BstAPI 3286 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2047 TTCGAA TT^CGAA
BstXI 2988 CCAAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3186 CCTCAGG CC^T[ACGT]AGG
DraIII 826 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1699 GACCCGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1256 TGGCCA [CT]^GGCC[GA]
Eco47III 2838 AGCGCT AGC^GCT
Eco81I 3186 CCTCAGG CC^T[ACGT]AGG
EcoRI 2044 GAATTC G^AATTC
FspI 630 TGCGCA TGC^GCA
HaeII 2838 AGCGCT [GA]GCGC^[CT]
KspI 3882 CCGCGG CCGC^GG
MamI 358 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1256 TGGCCA TGG^CCA
MscI 1256 TGGCCA TGG^CCA
NaeI 2210 GCCGGC GCC^GGC
NcoI 1766 CCATGG C^CATGG
NgoMIV 2210 GCCGGC G^CCGGC
NspV 2047 TTCGAA TT^CGAA
PmaCI 2284 CACGTG CAC^GTG
PmlI 2284 CACGTG CAC^GTG
PshAI 1826 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3882 CCGCGG CCGC^GG
SalI 781 GTCGAC G^TCGAC
SfuI 2047 TTCGAA TT^CGAA
SnaBI 3256 TACGTA TAC^GTA
SphI 627 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 781 GTCGAC GT^[AC][GT]AC
3178 GTCTAC
AccIII 751 TCCGGA T^CCGGA
2627 TCCGGA
AhdI 2095 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3013 GACCGTGAGTC
Asp700I 3619 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3640 GAACCGTTTC
AspEI 2095 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3013 GACCGTGAGTC
BamHI 1089 GGATCC G^GATCC
2419 GGATCC
BbvCI 2841 GCTGAGG CCTCAGC(-5/-2)
3520 GCTGAGG
BsaWI 751 TCCGGA [AT]^CCGG[AT]
2627 TCCGGA
BseAI 751 TCCGGA T^CCGGA
2627 TCCGGA
Bsp1407I 893 TGTACA T^GTACA
1865 TGTACA
BspEI 751 TCCGGA T^CCGGA
2627 TCCGGA
BspHI 3615 TCATGA T^CATGA
4085 TCATGA
BsrFI 785 ACCGGC [GA]^CCGG[CT]
2210 GCCGGC
BsrGI 893 TGTACA T^GTACA
1865 TGTACA
Cfr10I 785 ACCGGC [GA]^CCGG[CT]
2210 GCCGGC
DraI 219 TTTAAA TTT^AAA
4093 TTTAAA
Eam1105I 2095 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3013 GACCGTGAGTC
HincII 781 GTCGAC GT[CT]^[GA]AC
3862 GTCAAC
HindII 781 GTCGAC GT[CT]^[GA]AC
3862 GTCAAC
HindIII 2409 AAGCTT A^AGCTT
4044 AAGCTT
MroI 751 TCCGGA T^CCGGA
2627 TCCGGA
PvuII 301 CAGCTG CAG^CTG
2976 CAGCTG
RcaI 3615 TCATGA T^CATGA
4085 TCATGA
SacI 3582 GAGCTC GAGCT^C
3691 GAGCTC
SapI 1281 GAAGAGC GCTCTTC(1/4)
1400 GCTCTTC
SmlI 3579 CTTGAG C^T[CT][GA]AG
3756 CTCAAG
SspBI 893 TGTACA T^GTACA
1865 TGTACA
SspI 3622 AATATT AAT^ATT
4107 AATATT
XcmI 331 CCAGACTCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2985 CCACCAAGACCGTGG
XmnI 3619 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3640 GAACCGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BclI BcnI BfaI
BglI BmyI BpmI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI