##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII BalI BbeI BbrPI BbvCI
BcgI BciVI BglI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FseI FspI
HaeII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AciI 1531 CCGC CCGC(-3/-1)
AcyI 154 GACGCC G[GA]^CG[CT]C
AflII 2854 CTTAAG C^TTAAG
AlwNI 1086 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3017 ATTAAT AT^TAAT
AsnI 3017 ATTAAT AT^TAAT
Asp700I 947 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1133 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1495 CCTAGG C^CTAGG
BamHI 1856 GGATCC G^GATCC
BfrI 2854 CTTAAG C^TTAAG
BlnI 1495 CCTAGG C^CTAGG
BsaHI 154 GACGCC G[GA]^CG[CT]C
BsaI 885 GAGACC GGTCTC(1/5)
BsmBI 1413 CGTCTC CGTCTC(1/5)
Bsp1407I 2144 TGTACA T^GTACA
BspMI 3071 ACCTGC ACCTGC(4/8)
BsrDI 1826 CATTGC GCAATG(2/0)
BsrGI 2144 TGTACA T^GTACA
BssSI 1453 CACGAG CACGAG(-5/-1)
BstAPI 59 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1370 CCAGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 176 GCGC GCG^C
DsaI 294 CCATGG C^C[GA][CT]GG
EaeI 1430 CGGCCA [CT]^GGCC[GA]
EcoRV 835 GATATC GAT^ATC
EcoT22I 1990 ATGCAT ATGCA^T
FauI 1530 CCCGC CCCGC(4/6)
HgaI 154 GACGC GACGC(5/10)
HhaI 176 GCGC GCG^C
Hin1I 154 GACGCC G[GA]^CG[CT]C
HinP1I 176 GCGC G^CGC
MaeII 1691 ACGT A^CGT
MslI 2189 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1308 CAGCTG C[AC]G^C[GT]G
NcoI 294 CCATGG C^CATGG
NdeI 3324 CATATG CA^TATG
NsiI 1990 ATGCAT ATGCA^T
PflFI 1133 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3017 ATTAAT AT^TAAT
PstI 66 CTGCAG CTGCA^G
PvuII 1308 CAGCTG CAG^CTG
SacI 2198 GAGCTC GAGCT^C
SfcI 66 CTGCAG C^T[GA][CT]AG
SspBI 2144 TGTACA T^GTACA
SspI 3046 AATATT AAT^ATT
StuI 1457 AGGCCT AGG^CCT
TaqI 1669 TCGA T^CGA
Tth111I 1133 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1669 TCGA T^CGA
XbaI 1346 TCTAGA T^CTAGA
XcmI 159 CCACAAGTCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 947 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 2842 GTAGAC GT^[AC][GT]AC
3206 GTCTAC
AflIII 423 ACATGT A^C[GA][CT]GT
787 ACATGT
AocI 42 CCTCAGG CC^T[ACGT]AGG
2105 CCTAAGG
BanI 703 GGTGCC G^G[CT][GA]CC
980 GGTGCC
BanII 299 GAGCCC G[GA]GC[CT]^C
2198 GAGCTC
BcnI 990 CCCGG CC^[GC]GG
1569 CCCGG
BspHI 418 TCATGA T^CATGA
855 TCATGA
BspLU11I 423 ACATGT A^CATGT
787 ACATGT
Bsu36I 42 CCTCAGG CC^T[ACGT]AGG
2105 CCTAAGG
Eco81I 42 CCTCAGG CC^T[ACGT]AGG
2105 CCTAAGG
HincII 238 GTCAAC GT[CT]^[GA]AC
1382 GTCAAC
HindII 238 GTCAAC GT[CT]^[GA]AC
1382 GTCAAC
NciI 990 CCCGG CC^[GC]GG
1569 CCCGG
RcaI 418 TCATGA T^CATGA
855 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbsI BbvI BclI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoNI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII