##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AvaI        
AviII       BalI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BglI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmI        BsoBI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       FseI        FspI        
HaeII       HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SwaI        Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AciI         1531  CCGC                 CCGC(-3/-1)
AcyI          154  GACGCC               G[GA]^CG[CT]C
AflII        2854  CTTAAG               C^TTAAG
AlwNI        1086  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI         3017  ATTAAT               AT^TAAT
AsnI         3017  ATTAAT               AT^TAAT
Asp700I       947  GAACTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1133  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1495  CCTAGG               C^CTAGG
BamHI        1856  GGATCC               G^GATCC
BfrI         2854  CTTAAG               C^TTAAG
BlnI         1495  CCTAGG               C^CTAGG
BsaHI         154  GACGCC               G[GA]^CG[CT]C
BsaI          885  GAGACC               GGTCTC(1/5)
BsmBI        1413  CGTCTC               CGTCTC(1/5)
Bsp1407I     2144  TGTACA               T^GTACA
BspMI        3071  ACCTGC               ACCTGC(4/8)
BsrDI        1826  CATTGC               GCAATG(2/0)
BsrGI        2144  TGTACA               T^GTACA
BssSI        1453  CACGAG               CACGAG(-5/-1)
BstAPI         59  GCACTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1370  CCAGTTTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          176  GCGC                 GCG^C
DsaI          294  CCATGG               C^C[GA][CT]GG
EaeI         1430  CGGCCA               [CT]^GGCC[GA]
EcoRV         835  GATATC               GAT^ATC
EcoT22I      1990  ATGCAT               ATGCA^T
FauI         1530  CCCGC                CCCGC(4/6)
HgaI          154  GACGC                GACGC(5/10)
HhaI          176  GCGC                 GCG^C
Hin1I         154  GACGCC               G[GA]^CG[CT]C
HinP1I        176  GCGC                 G^CGC
MaeII        1691  ACGT                 A^CGT
MslI         2189  CATTAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1308  CAGCTG               C[AC]G^C[GT]G
NcoI          294  CCATGG               C^CATGG
NdeI         3324  CATATG               CA^TATG
NsiI         1990  ATGCAT               ATGCA^T
PflFI        1133  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        3017  ATTAAT               AT^TAAT
PstI           66  CTGCAG               CTGCA^G
PvuII        1308  CAGCTG               CAG^CTG
SacI         2198  GAGCTC               GAGCT^C
SfcI           66  CTGCAG               C^T[GA][CT]AG
SspBI        2144  TGTACA               T^GTACA
SspI         3046  AATATT               AAT^ATT
StuI         1457  AGGCCT               AGG^CCT
TaqI         1669  TCGA                 T^CGA
Tth111I      1133  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1669  TCGA                 T^CGA
XbaI         1346  TCTAGA               T^CTAGA
XcmI          159  CCACAAGTCTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          947  GAACTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI         2842  GTAGAC               GT^[AC][GT]AC
             3206  GTCTAC              
AflIII        423  ACATGT               A^C[GA][CT]GT
              787  ACATGT              
AocI           42  CCTCAGG              CC^T[ACGT]AGG
             2105  CCTAAGG             
BanI          703  GGTGCC               G^G[CT][GA]CC
              980  GGTGCC              
BanII         299  GAGCCC               G[GA]GC[CT]^C
             2198  GAGCTC              
BcnI          990  CCCGG                CC^[GC]GG
             1569  CCCGG               
BspHI         418  TCATGA               T^CATGA
              855  TCATGA              
BspLU11I      423  ACATGT               A^CATGT
              787  ACATGT              
Bsu36I         42  CCTCAGG              CC^T[ACGT]AGG
             2105  CCTAAGG             
Eco81I         42  CCTCAGG              CC^T[ACGT]AGG
             2105  CCTAAGG             
HincII        238  GTCAAC               GT[CT]^[GA]AC
             1382  GTCAAC              
HindII        238  GTCAAC               GT[CT]^[GA]AC
             1382  GTCAAC              
NciI          990  CCCGG                CC^[GC]GG
             1569  CCCGG               
RcaI          418  TCATGA               T^CATGA
              855  TCATGA              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AspHI       AvaII       BbsI        BbvI        BclI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII