##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AspI
AvrII BbeI BbrPI BbvCI BcgI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
BstAPI BstEII BstPI CelII ClaI
CpoI DraII DraIII EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoO65I
EcoRI FseI Hin1I KasI KpnI
MaeII MluI MroI MslI NaeI
NarI NgoMIV NheI NotI NruI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SspBI SwaI Tth111I Van91I XcmI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 4032 GTATAC GT^[AC][GT]AC
AocI 2919 CCTGAGG CC^T[ACGT]AGG
Aor51HI 176 AGCGCT AGC^GCT
AviII 2290 TGCGCA TGC^GCA
BalI 2770 TGGCCA TGG^CCA
BamHI 2672 GGATCC G^GATCC
BanI 1716 GGTGCC G^G[CT][GA]CC
BciVI 3395 GGATAC GTATCC(6/5)
BclI 2612 TGATCA T^GATCA
BglI 217 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 3073 GAGACC GGTCTC(1/5)
BsgI 1793 GTGCAG GTGCAG(16/14)
BsmI 3578 GCATTC GAATGC(1/-1)
BspMI 431 GCAGGT ACCTGC(4/8)
BsrBI 196 GAGCGG CCGCTC(-3/-3)
BsrDI 1168 GCAATG GCAATG(2/0)
BssHII 282 GCGCGC G^CGCGC
BssSI 2885 CACGAG CACGAG(-5/-1)
Bst1107I 4032 GTATAC GTA^TAC
BstBI 2431 TTCGAA TT^CGAA
BstXI 945 CCAAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4032 GTATAC GTA^TAC
Bsu36I 2919 CCTGAGG CC^T[ACGT]AGG
DrdI 3740 GACTTATTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 176 AGCGCT AGC^GCT
Eco81I 2919 CCTGAGG CC^T[ACGT]AGG
EcoRV 2178 GATATC GAT^ATC
EcoT22I 3576 ATGCAT ATGCA^T
FbaI 2612 TGATCA T^GATCA
FspI 2290 TGCGCA TGC^GCA
HgaI 1401 GACGC GACGC(5/10)
HpaI 885 GTTAAC GTT^AAC
KspI 242 CCGCGG CCGC^GG
MfeI 1427 CAATTG C^AATTG
MluNI 2770 TGGCCA TGG^CCA
MscI 2770 TGGCCA TGG^CCA
MunI 1427 CAATTG C^AATTG
NdeI 3721 CATATG CA^TATG
NsiI 3576 ATGCAT ATGCA^T
NspV 2431 TTCGAA TT^CGAA
PacI 693 TTAATTAA TTAAT^TAA
PaeR7I 193 CTCGAG C^TCGAG
PvuII 3988 CAGCTG CAG^CTG
SacI 1340 GAGCTC GAGCT^C
SacII 242 CCGCGG CCGC^GG
SapI 999 GAAGAGC GCTCTTC(1/4)
SfuI 2431 TTCGAA TT^CGAA
SmaI 120 CCCGGG CCC^GGG
SpeI 2205 ACTAGT A^CTAGT
StuI 2077 AGGCCT AGG^CCT
XbaI 3836 TCTAGA T^CTAGA
XhoI 193 CTCGAG C^TCGAG
XmaCI 120 CCCGGG C^CCGGG
XmaI 120 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AvaI 120 CCCGGG C^[CT]CG[GA]G
193 CTCGAG
BglII 1077 AGATCT A^GATCT
4000 AGATCT
BseRI 2677 CTCCTC GAGGAG(10/8)
425 GAGGAG
BsoBI 120 CCCGGG C^[CT]CG[GA]G
193 CTCGAG
BspHI 3069 TCATGA T^CATGA
3807 TCATGA
BsrFI 286 GCCGGT [GA]^CCGG[CT]
2495 ACCGGC
Cfr10I 286 GCCGGT [GA]^CCGG[CT]
2495 ACCGGC
NcoI 994 CCATGG C^CATGG
2773 CCATGG
RcaI 3069 TCATGA T^CATGA
3807 TCATGA
SmlI 193 CTCGAG C^T[CT][GA]AG
1320 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI