##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BbrPI BcgI
BclI BcnI BfrI BglI BlnI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII BstEII BstPI BstUI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI FauI FbaI FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 2644 ACATGT A^C[GA][CT]GT
AlwNI 2676 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 957 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 558 CCCGAG C^[CT]CG[GA]G
BalI 1999 TGGCCA TGG^CCA
BamHI 2843 GGATCC G^GATCC
BanII 3170 GAGCCC G[GA]GC[CT]^C
BbvCI 331 CCTCAGC CCTCAGC(-5/-2)
BciVI 1905 GTATCC GTATCC(6/5)
BsaAI 3891 CACGTA [CT]AC^GT[GA]
BsaBI 3898 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 798 TCCGGT [AT]^CCGG[AT]
BseRI 2684 GAGGAG GAGGAG(10/8)
BsoBI 558 CCCGAG C^[CT]CG[GA]G
BspLU11I 2644 ACATGT A^CATGT
BssSI 3472 CACGAG CACGAG(-5/-1)
Bst1107I 1924 GTATAC GTA^TAC
BstAPI 582 GCAAGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1307 TTCGAA TT^CGAA
BstZ17I 1924 GTATAC GTA^TAC
DraI 26 TTTAAA TTT^AAA
EcoRV 1718 GATATC GAT^ATC
MamI 3898 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3117 CAATTG C^AATTG
MluNI 1999 TGGCCA TGG^CCA
MscI 1999 TGGCCA TGG^CCA
MunI 3117 CAATTG C^AATTG
NdeI 1430 CATATG CA^TATG
NheI 2278 GCTAGC G^CTAGC
NspV 1307 TTCGAA TT^CGAA
PflFI 957 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 3319 AGTACT AGT^ACT
SexAI 2897 ACCTGGT A^CC[AT]GGT
SfuI 1307 TTCGAA TT^CGAA
SspI 292 AATATT AAT^ATT
StuI 3216 AGGCCT AGG^CCT
Tth111I 957 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2698 CCAAAAAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1924 GTATAC GT^[AC][GT]AC
3620 GTAGAC
Alw44I 421 GTGCAC G^TGCAC
1301 GTGCAC
AocI 543 CCTTAGG CC^T[ACGT]AGG
2663 CCTGAGG
Aor51HI 143 AGCGCT AGC^GCT
3078 AGCGCT
ApaLI 421 GTGCAC G^TGCAC
1301 GTGCAC
Asp700I 473 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2723 GAAACTATTC
AspHI 421 GTGCAC G[AT]GC[AT]^C
1301 GTGCAC
BglII 3989 AGATCT A^GATCT
4067 AGATCT
BlpI 3167 GCTGAGC GC^T[ACGT]AGC
3186 GCTTAGC
BpmI 1674 CTCCAG CTGGAG(16/14)
3273 CTCCAG
Bpu1102I 3167 GCTGAGC GC^T[ACGT]AGC
3186 GCTTAGC
BsiHKAI 421 GTGCAC G[AT]GC[AT]^C
1301 GTGCAC
BsmI 553 GAATGC GAATGC(1/-1)
3058 GAATGC
BspMI 1195 ACCTGC ACCTGC(4/8)
2876 ACCTGC
Bsu36I 543 CCTTAGG CC^T[ACGT]AGG
2663 CCTGAGG
CelII 3167 GCTGAGC GC^T[ACGT]AGC
3186 GCTTAGC
Eco47III 143 AGCGCT AGC^GCT
3078 AGCGCT
Eco81I 543 CCTTAGG CC^T[ACGT]AGG
2663 CCTGAGG
EcoNI 1217 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2515 CCTTGGGAAGG
HaeII 143 AGCGCT [GA]GCGC^[CT]
3078 AGCGCT
HapII 799 CCGG C^CGG
3192 CCGG
HincII 869 GTCAAC GT[CT]^[GA]AC
2783 GTTGAC
HindII 869 GTCAAC GT[CT]^[GA]AC
2783 GTTGAC
HindIII 615 AAGCTT A^AGCTT
2218 AAGCTT
HpaII 799 CCGG C^CGG
3192 CCGG
MaeII 649 ACGT A^CGT
3892 ACGT
MspI 799 CCGG C^CGG
3192 CCGG
PflMI 1600 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2286 CCAGTTTATGG
SapI 748 GAAGAGC GCTCTTC(1/4)
2153 GAAGAGC
SmlI 738 CTCAAG C^T[CT][GA]AG
1740 CTCAAG
Van91I 1600 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2286 CCAGTTTATGG
XmnI 473 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2723 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII