##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AviII AvrII BamHI BcgI BfrI
BlnI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI DraI EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KpnI MamI MfeI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI SacI SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 1840 GAATTT [GA]^AATT[CT]
AflIII 3746 ACGCGT A^C[GA][CT]GT
Alw44I 1401 GTGCAC G^TGCAC
ApaLI 1401 GTGCAC G^TGCAC
ApoI 1840 GAATTT [GA]^AATT[CT]
Asp700I 178 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 3105 GAAGAC GAAGAC(2/6)
BclI 1435 TGATCA T^GATCA
BglII 3248 AGATCT A^GATCT
BlpI 748 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 748 GCTCAGC GC^T[ACGT]AGC
BpuAI 3105 GAAGAC GAAGAC(2/6)
BsiEI 3938 CGGCCG CG[GA][CT]^CG
BstEII 1488 GGTCACC G^GT[ACGT]ACC
BstPI 1488 GGTCACC G^GT[ACGT]ACC
BstXI 3483 CCACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3248 AGATCT [GA]^GATC[CT]
CelII 748 GCTCAGC GC^T[ACGT]AGC
CpoI 3710 CGGACCG CG^G[AT]CCG
EagI 3938 CGGCCG C^GGCCG
EarI 1205 CTCTTC CTCTTC(1/4)
EclXI 3938 CGGCCG C^GGCCG
Eco52I 3938 CGGCCG C^GGCCG
EcoO65I 1488 GGTCACC G^GT[ACGT]ACC
FbaI 1435 TGATCA T^GATCA
Ksp632I 1205 CTCTTC CTCTTC(1/4)
MflI 3248 AGATCT [GA]^GATC[CT]
MluI 3746 ACGCGT A^CGCGT
MslI 4022 CACGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1780 GCTAGC G^CTAGC
PaeR7I 802 CTCGAG C^TCGAG
RsrII 3710 CGGACCG CG^G[AT]CCG
SbfI 1426 CCTGCAGG CCTGCA^GG
SpeI 2984 ACTAGT A^CTAGT
Sse8387I 1426 CCTGCAGG CCTGCA^GG
TaqI 803 TCGA T^CGA
TfiI 629 GATTC G^A[AT]TC
TthHB8I 803 TCGA T^CGA
XbaI 1872 TCTAGA T^CTAGA
XhoI 802 CTCGAG C^TCGAG
XhoII 3248 AGATCT [GA]^GATC[CT]
XmnI 178 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1366 GACGTC GACGT^C
1411 GACGTC
AlwI 820 GGATC GGATC(4/5)
2590 GGATC
AocI 298 CCTTAGG CC^T[ACGT]AGG
4077 CCTCAGG
Aor51HI 2663 AGCGCT AGC^GCT
3422 AGCGCT
AspI 1411 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4228 GACAGCGTC
BbeI 235 GGCGCC GGCGC^C
1343 GGCGCC
BbrPI 88 CACGTG CAC^GTG
215 CACGTG
BciVI 998 GGATAC GTATCC(6/5)
60 GTATCC
BsaI 2555 GAGACC GGTCTC(1/5)
2467 GGTCTC
Bsp1407I 36 TGTACA T^GTACA
552 TGTACA
BsrDI 2574 CATTGC GCAATG(2/0)
1449 GCAATG
BsrGI 36 TGTACA T^GTACA
552 TGTACA
Bsu36I 298 CCTTAGG CC^T[ACGT]AGG
4077 CCTCAGG
DraIII 2481 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3052 CACCGTGTG
DrdI 1214 GACTCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1366 GACGTCACTGTC
Eco47III 2663 AGCGCT AGC^GCT
3422 AGCGCT
Eco81I 298 CCTTAGG CC^T[ACGT]AGG
4077 CCTCAGG
KasI 235 GGCGCC G^GCGCC
1343 GGCGCC
KspI 166 CCGCGG CCGC^GG
1406 CCGCGG
NaeI 565 GCCGGC GCC^GGC
807 GCCGGC
NarI 235 GGCGCC GG^CGCC
1343 GGCGCC
NgoMIV 565 GCCGGC G^CCGGC
807 GCCGGC
PflFI 1411 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4228 GACAGCGTC
PmaCI 88 CACGTG CAC^GTG
215 CACGTG
PmlI 88 CACGTG CAC^GTG
215 CACGTG
SacII 166 CCGCGG CCGC^GG
1406 CCGCGG
SfoI 235 GGCGCC GGC^GCC
1343 GGCGCC
SphI 721 GCATGC GCATG^C
4118 GCATGC
SspBI 36 TGTACA T^GTACA
552 TGTACA
Tsp509I 1841 AATT ^AATT
4288 AATT
Tth111I 1411 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4228 GACAGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwNI ApaI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI Tru9I TseI
Tsp45I TspRI Van91I XcmI XmaCI
XmaI