##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BciVI
BlnI BsaBI BseAI BsiWI BspEI
BspLU11I BssHII BstAPI CpoI EcoRV
FseI FspI HpaI KasI KpnI
KspI MamI MluI MroI NarI
NdeI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SgrAI SmaI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1634 GACGTC GACGT^C
AflIII 2156 ACGTGT A^C[GA][CT]GT
AhdI 3154 GACAGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2595 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2898 AGCGCT AGC^GCT
AspEI 3154 GACAGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1696 CACGTG CAC^GTG
BbvCI 1251 GCTGAGG CCTCAGC(-5/-2)
BglII 1096 AGATCT A^GATCT
BlpI 2444 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2444 GCTTAGC GC^T[ACGT]AGC
BsmBI 455 CGTCTC CGTCTC(1/5)
BspDI 4248 ATCGAT AT^CGAT
BspHI 2166 TCATGA T^CATGA
Bst1107I 497 GTATAC GTA^TAC
BstBI 804 TTCGAA TT^CGAA
BstEII 167 GGTCACC G^GT[ACGT]ACC
BstPI 167 GGTCACC G^GT[ACGT]ACC
BstZ17I 497 GTATAC GTA^TAC
Bsu36I 2595 CCTGAGG CC^T[ACGT]AGG
CelII 2444 GCTTAGC GC^T[ACGT]AGC
ClaI 4248 ATCGAT AT^CGAT
DraIII 170 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 437 GACAGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 432 CGGCCG C^GGCCG
Eam1105I 3154 GACAGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 432 CGGCCG C^GGCCG
Eco47III 2898 AGCGCT AGC^GCT
Eco52I 432 CGGCCG C^GGCCG
Eco81I 2595 CCTGAGG CC^T[ACGT]AGG
EcoNI 3309 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 167 GGTCACC G^GT[ACGT]ACC
EcoRI 1011 GAATTC G^AATTC
EcoT22I 4296 ATGCAT ATGCA^T
HindIII 4406 AAGCTT A^AGCTT
MfeI 1712 CAATTG C^AATTG
MunI 1712 CAATTG C^AATTG
NheI 3789 GCTAGC G^CTAGC
NsiI 4296 ATGCAT ATGCA^T
NspI 2389 GCATGT [GA]CATG^[CT]
NspV 804 TTCGAA TT^CGAA
PmaCI 1696 CACGTG CAC^GTG
PmlI 1696 CACGTG CAC^GTG
RcaI 2166 TCATGA T^CATGA
SalI 1637 GTCGAC G^TCGAC
ScaI 907 AGTACT AGT^ACT
SfuI 804 TTCGAA TT^CGAA
SnaBI 1477 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 497 GTATAC GT^[AC][GT]AC
1637 GTCGAC
AccII 211 CGCG CG^CG
1028 CGCG
AclI 1726 AACGTT AA^CGTT
2630 AACGTT
AcyI 1634 GACGTC G[GA]^CG[CT]C
3767 GACGCC
AflII 2282 CTTAAG C^TTAAG
4363 CTTAAG
ApaI 686 GGGCCC GGGCC^C
822 GGGCCC
BalI 236 TGGCCA TGG^CCA
1160 TGGCCA
BcgI 1068 GCACCCGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1387 GCACGCTGGTCG
BfrI 2282 CTTAAG C^TTAAG
4363 CTTAAG
BsaAI 1477 TACGTA [CT]AC^GT[GA]
1696 CACGTG
BsaHI 1634 GACGTC G[GA]^CG[CT]C
3767 GACGCC
BsaWI 288 TCCGGT [AT]^CCGG[AT]
1908 ACCGGA
BsrBI 312 GAGCGG CCGCTC(-3/-3)
1182 GAGCGG
BssSI 1343 CACGAG CACGAG(-5/-1)
3008 CTCGTG
BstUI 211 CGCG CG^CG
1028 CGCG
HaeII 1538 AGCGCC [GA]GCGC^[CT]
2898 AGCGCT
Hin1I 1634 GACGTC G[GA]^CG[CT]C
3767 GACGCC
MluNI 236 TGGCCA TGG^CCA
1160 TGGCCA
MscI 236 TGGCCA TGG^CCA
1160 TGGCCA
MvnI 211 CGCG CG^CG
1028 CGCG
NaeI 618 GCCGGC GCC^GGC
1513 GCCGGC
NgoMIV 618 GCCGGC G^CCGGC
1513 GCCGGC
Psp1406I 1726 AACGTT AA^CGTT
2630 AACGTT
SacI 1593 GAGCTC GAGCT^C
2207 GAGCTC
SapI 3143 GAAGAGC GCTCTTC(1/4)
3746 GCTCTTC
XbaI 2329 TCTAGA T^CTAGA
3163 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI