##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI AspEI
AvrII BbvCI BciVI BglI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BssHII BssSI BstEII BstPI Bsu36I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI FseI HincII HindII
HindIII HpaI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PshAI Psp1406I PvuI RcaI RsrII
SacI SalI SfiI SgrAI SnaBI
SpeI StuI SwaI XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 2428 GTATAC GT^[AC][GT]AC
AflII 3583 CTTAAG C^TTAAG
Aor51HI 272 AGCGCT AGC^GCT
ApaI 653 GGGCCC GGGCC^C
AseI 3890 ATTAAT AT^TAAT
AsnI 3890 ATTAAT AT^TAAT
AspI 2212 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 798 TGCGCA TGC^GCA
BamHI 2052 GGATCC G^GATCC
BanII 653 GGGCCC G[GA]GC[CT]^C
BclI 2320 TGATCA T^GATCA
BfrI 3583 CTTAAG C^TTAAG
BsaBI 500 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 3338 GAGGAG GAGGAG(10/8)
BsiEI 736 CGGTCG CG[GA][CT]^CG
BspLU11I 57 ACATGT A^CATGT
BsrBI 294 GAGCGG CCGCTC(-3/-3)
BsrDI 3927 GCAATG GCAATG(2/0)
BsrFI 1607 ACCGGC [GA]^CCGG[CT]
Bst1107I 2428 GTATAC GTA^TAC
BstBI 975 TTCGAA TT^CGAA
BstXI 1246 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2428 GTATAC GTA^TAC
Cfr10I 1607 ACCGGC [GA]^CCGG[CT]
Eco47III 272 AGCGCT AGC^GCT
EcoRV 3544 GATATC GAT^ATC
EcoT22I 1094 ATGCAT ATGCA^T
FbaI 2320 TGATCA T^GATCA
FspI 798 TGCGCA TGC^GCA
KspI 642 CCGCGG CCGC^GG
MamI 500 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 1094 ATGCAT ATGCA^T
NspV 975 TTCGAA TT^CGAA
PflFI 2212 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 1132 GTTTAAAC GTTT^AAAC
PpuMI 746 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 3890 ATTAAT AT^TAAT
SacII 642 CCGCGG CCGC^GG
SapI 2840 GCTCTTC GCTCTTC(1/4)
SbfI 406 CCTGCAGG CCTGCA^GG
ScaI 1997 AGTACT AGT^ACT
SfuI 975 TTCGAA TT^CGAA
SmaI 650 CCCGGG CCC^GGG
Sse8387I 406 CCTGCAGG CCTGCA^GG
Tth111I 2212 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 650 CCCGGG C^CCGGG
XmaI 650 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 918 GGTACC G^GTACC
2145 GGTACC
Asp700I 1433 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3722 GAAACTATTC
Asp718I 918 GGTACC G^GTACC
2145 GGTACC
BalI 210 TGGCCA TGG^CCA
2604 TGGCCA
BbeI 2630 GGCGCC GGCGC^C
2638 GGCGCC
BcgI 977 CGAAACATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
730 GCACCCCGGTCG
BsgI 771 GTGCAG GTGCAG(16/14)
2672 GTGCAG
BsmBI 146 GAGACG CGTCTC(1/5)
1353 GAGACG
EarI 1013 CTCTTC CTCTTC(1/4)
2841 CTCTTC
KasI 2630 GGCGCC G^GCGCC
2638 GGCGCC
KpnI 918 GGTACC GGTAC^C
2145 GGTACC
Ksp632I 1013 CTCTTC CTCTTC(1/4)
2841 CTCTTC
MluNI 210 TGGCCA TGG^CCA
2604 TGGCCA
MscI 210 TGGCCA TGG^CCA
2604 TGGCCA
NarI 2630 GGCGCC GG^CGCC
2638 GGCGCC
NcoI 1252 CCATGG C^CATGG
3090 CCATGG
PflMI 2383 CCAAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2642 CCACCTGTTGG
SexAI 965 ACCAGGT A^CC[AT]GGT
2148 ACCTGGT
SfoI 2630 GGCGCC GGC^GCC
2638 GGCGCC
SmlI 1993 CTTGAG C^T[CT][GA]AG
3583 CTTAAG
Van91I 2383 CCAAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2642 CCACCTGTTGG
XmnI 1433 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3722 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BbrPI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII