################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I AocI ApaLI AscI AspEI AvrII BbvCI BciVI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BssHII BssSI BstEII BstPI Bsu36I CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI FseI HincII HindII HindIII HpaI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SfiI SgrAI SnaBI SpeI StuI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 2428 GTATAC GT^[AC][GT]AC AflII 3583 CTTAAG C^TTAAG Aor51HI 272 AGCGCT AGC^GCT ApaI 653 GGGCCC GGGCC^C AseI 3890 ATTAAT AT^TAAT AsnI 3890 ATTAAT AT^TAAT AspI 2212 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 798 TGCGCA TGC^GCA BamHI 2052 GGATCC G^GATCC BanII 653 GGGCCC G[GA]GC[CT]^C BclI 2320 TGATCA T^GATCA BfrI 3583 CTTAAG C^TTAAG BsaBI 500 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 3338 GAGGAG GAGGAG(10/8) BsiEI 736 CGGTCG CG[GA][CT]^CG BspLU11I 57 ACATGT A^CATGT BsrBI 294 GAGCGG CCGCTC(-3/-3) BsrDI 3927 GCAATG GCAATG(2/0) BsrFI 1607 ACCGGC [GA]^CCGG[CT] Bst1107I 2428 GTATAC GTA^TAC BstBI 975 TTCGAA TT^CGAA BstXI 1246 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2428 GTATAC GTA^TAC Cfr10I 1607 ACCGGC [GA]^CCGG[CT] Eco47III 272 AGCGCT AGC^GCT EcoRV 3544 GATATC GAT^ATC EcoT22I 1094 ATGCAT ATGCA^T FbaI 2320 TGATCA T^GATCA FspI 798 TGCGCA TGC^GCA KspI 642 CCGCGG CCGC^GG MamI 500 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NsiI 1094 ATGCAT ATGCA^T NspV 975 TTCGAA TT^CGAA PflFI 2212 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmeI 1132 GTTTAAAC GTTT^AAAC PpuMI 746 GGGACCC [GA]G^G[AT]CC[CT] PshBI 3890 ATTAAT AT^TAAT SacII 642 CCGCGG CCGC^GG SapI 2840 GCTCTTC GCTCTTC(1/4) SbfI 406 CCTGCAGG CCTGCA^GG ScaI 1997 AGTACT AGT^ACT SfuI 975 TTCGAA TT^CGAA SmaI 650 CCCGGG CCC^GGG Sse8387I 406 CCTGCAGG CCTGCA^GG Tth111I 2212 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 650 CCCGGG C^CCGGG XmaI 650 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 918 GGTACC G^GTACC 2145 GGTACC Asp700I 1433 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3722 GAAACTATTC Asp718I 918 GGTACC G^GTACC 2145 GGTACC BalI 210 TGGCCA TGG^CCA 2604 TGGCCA BbeI 2630 GGCGCC GGCGC^C 2638 GGCGCC BcgI 977 CGAAACATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 730 GCACCCCGGTCG BsgI 771 GTGCAG GTGCAG(16/14) 2672 GTGCAG BsmBI 146 GAGACG CGTCTC(1/5) 1353 GAGACG EarI 1013 CTCTTC CTCTTC(1/4) 2841 CTCTTC KasI 2630 GGCGCC G^GCGCC 2638 GGCGCC KpnI 918 GGTACC GGTAC^C 2145 GGTACC Ksp632I 1013 CTCTTC CTCTTC(1/4) 2841 CTCTTC MluNI 210 TGGCCA TGG^CCA 2604 TGGCCA MscI 210 TGGCCA TGG^CCA 2604 TGGCCA NarI 2630 GGCGCC GG^CGCC 2638 GGCGCC NcoI 1252 CCATGG C^CATGG 3090 CCATGG PflMI 2383 CCAAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2642 CCACCTGTTGG SexAI 965 ACCAGGT A^CC[AT]GGT 2148 ACCTGGT SfoI 2630 GGCGCC GGC^GCC 2638 GGCGCC SmlI 1993 CTTGAG C^T[CT][GA]AG 3583 CTTAAG Van91I 2383 CCAAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2642 CCACCTGTTGG XmnI 1433 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3722 GAAACTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII