##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        ApaLI       AscI        AspEI       
AvrII       BbvCI       BciVI       BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaWI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BssHII      BssSI       BstEII      BstPI       Bsu36I      
CelII       ClaI        CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       FseI        HincII      HindII      
HindIII     HpaI        MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SfiI        SgrAI       SnaBI       
SpeI        StuI        SwaI        XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         2428  GTATAC               GT^[AC][GT]AC
AflII        3583  CTTAAG               C^TTAAG
Aor51HI       272  AGCGCT               AGC^GCT
ApaI          653  GGGCCC               GGGCC^C
AseI         3890  ATTAAT               AT^TAAT
AsnI         3890  ATTAAT               AT^TAAT
AspI         2212  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         798  TGCGCA               TGC^GCA
BamHI        2052  GGATCC               G^GATCC
BanII         653  GGGCCC               G[GA]GC[CT]^C
BclI         2320  TGATCA               T^GATCA
BfrI         3583  CTTAAG               C^TTAAG
BsaBI         500  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        3338  GAGGAG               GAGGAG(10/8)
BsiEI         736  CGGTCG               CG[GA][CT]^CG
BspLU11I       57  ACATGT               A^CATGT
BsrBI         294  GAGCGG               CCGCTC(-3/-3)
BsrDI        3927  GCAATG               GCAATG(2/0)
BsrFI        1607  ACCGGC               [GA]^CCGG[CT]
Bst1107I     2428  GTATAC               GTA^TAC
BstBI         975  TTCGAA               TT^CGAA
BstXI        1246  CCACCACCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2428  GTATAC               GTA^TAC
Cfr10I       1607  ACCGGC               [GA]^CCGG[CT]
Eco47III      272  AGCGCT               AGC^GCT
EcoRV        3544  GATATC               GAT^ATC
EcoT22I      1094  ATGCAT               ATGCA^T
FbaI         2320  TGATCA               T^GATCA
FspI          798  TGCGCA               TGC^GCA
KspI          642  CCGCGG               CCGC^GG
MamI          500  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI         1094  ATGCAT               ATGCA^T
NspV          975  TTCGAA               TT^CGAA
PflFI        2212  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmeI         1132  GTTTAAAC             GTTT^AAAC
PpuMI         746  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI        3890  ATTAAT               AT^TAAT
SacII         642  CCGCGG               CCGC^GG
SapI         2840  GCTCTTC              GCTCTTC(1/4)
SbfI          406  CCTGCAGG             CCTGCA^GG
ScaI         1997  AGTACT               AGT^ACT
SfuI          975  TTCGAA               TT^CGAA
SmaI          650  CCCGGG               CCC^GGG
Sse8387I      406  CCTGCAGG             CCTGCA^GG
Tth111I      2212  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         650  CCCGGG               C^CCGGG
XmaI          650  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I        918  GGTACC               G^GTACC
             2145  GGTACC              
Asp700I      1433  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3722  GAAACTATTC          
Asp718I       918  GGTACC               G^GTACC
             2145  GGTACC              
BalI          210  TGGCCA               TGG^CCA
             2604  TGGCCA              
BbeI         2630  GGCGCC               GGCGC^C
             2638  GGCGCC              
BcgI          977  CGAAACATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              730  GCACCCCGGTCG        
BsgI          771  GTGCAG               GTGCAG(16/14)
             2672  GTGCAG              
BsmBI         146  GAGACG               CGTCTC(1/5)
             1353  GAGACG              
EarI         1013  CTCTTC               CTCTTC(1/4)
             2841  CTCTTC              
KasI         2630  GGCGCC               G^GCGCC
             2638  GGCGCC              
KpnI          918  GGTACC               GGTAC^C
             2145  GGTACC              
Ksp632I      1013  CTCTTC               CTCTTC(1/4)
             2841  CTCTTC              
MluNI         210  TGGCCA               TGG^CCA
             2604  TGGCCA              
MscI          210  TGGCCA               TGG^CCA
             2604  TGGCCA              
NarI         2630  GGCGCC               GG^CGCC
             2638  GGCGCC              
NcoI         1252  CCATGG               C^CATGG
             3090  CCATGG              
PflMI        2383  CCAAATAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2642  CCACCTGTTGG         
SexAI         965  ACCAGGT              A^CC[AT]GGT
             2148  ACCTGGT             
SfoI         2630  GGCGCC               GGC^GCC
             2638  GGCGCC              
SmlI         1993  CTTGAG               C^T[CT][GA]AG
             3583  CTTAAG              
Van91I       2383  CCAAATAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2642  CCACCTGTTGG         
XmnI         1433  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3722  GAAACTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AspHI       AvaI        AvaII       BanI        BbrPI       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DrdI        DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PmaCI       PmlI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SphI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII