################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccIII AclI AcsI AgeI Aor51HI ApoI AscI AseI AsnI AviII AvrII BbeI BcgI BglII BlnI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoRI EcoRV FspI HpaI KasI KspI MfeI MroI MunI NarI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tsp509I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 419 GACGTC GACGT^C AflII 3598 CTTAAG C^TTAAG AhdI 512 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3182 GTGCAC G^TGCAC ApaLI 3182 GTGCAC G^TGCAC Asp700I 1342 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 512 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1729 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 317 CTCGGG C^[CT]CG[GA]G BamHI 2235 GGATCC G^GATCC BbrPI 1009 CACGTG CAC^GTG BbvCI 2416 CCTCAGC CCTCAGC(-5/-2) BclI 2004 TGATCA T^GATCA BfrI 3598 CTTAAG C^TTAAG BlpI 2763 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2763 GCTGAGC GC^T[ACGT]AGC BsaBI 534 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1836 TCCGGT [AT]^CCGG[AT] BsmI 2743 GAATGC GAATGC(1/-1) BsoBI 317 CTCGGG C^[CT]CG[GA]G BspHI 1752 TCATGA T^CATGA CelII 2763 GCTGAGC GC^T[ACGT]AGC DraIII 2177 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 512 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 2082 ATGCAT ATGCA^T FbaI 2004 TGATCA T^GATCA FseI 3976 GGCCGGCC GGCCGG^CC HaeII 2633 AGCGCC [GA]GCGC^[CT] HindIII 2864 AAGCTT A^AGCTT MamI 534 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 999 ACGCGT A^CGCGT NaeI 3977 GCCGGC GCC^GGC NgoMIV 3977 GCCGGC G^CCGGC NheI 3241 GCTAGC G^CTAGC NsiI 2082 ATGCAT ATGCA^T NspI 2481 ACATGC [GA]CATG^[CT] PflFI 1729 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1009 CACGTG CAC^GTG PmlI 1009 CACGTG CAC^GTG PshAI 1759 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1752 TCATGA T^CATGA SacI 1415 GAGCTC GAGCT^C ScaI 3383 AGTACT AGT^ACT SexAI 921 ACCTGGT A^CC[AT]GGT SfiI 3767 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 542 TCGA T^CGA Tth111I 1729 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 542 TCGA T^CGA XbaI 1822 TCTAGA T^CTAGA XmnI 1342 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Acc65I 61 GGTACC G^GTACC 3483 GGTACC AflIII 999 ACGCGT A^C[GA][CT]GT 1010 ACGTGT ApaI 201 GGGCCC GGGCC^C 4001 GGGCCC Asp718I 61 GGTACC G^GTACC 3483 GGTACC BciVI 1003 GTATCC GTATCC(6/5) 3196 GTATCC BcnI 994 CCGGG CC^[GC]GG 2953 CCGGG BglI 2012 GCCAAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3768 GCCTGGGGGGC BsaAI 1009 CACGTG [CT]AC^GT[GA] 2504 TACGTG BsrDI 490 CATTGC GCAATG(2/0) 1950 GCAATG BstEII 382 GGTCACC G^GT[ACGT]ACC 3255 GGTTACC BstPI 382 GGTCACC G^GT[ACGT]ACC 3255 GGTTACC BstXI 1872 CCATCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3898 CCATTGGTCTGG EcoO65I 382 GGTCACC G^GT[ACGT]ACC 3255 GGTTACC HincII 558 GTCAAC GT[CT]^[GA]AC 740 GTCAAC HindII 558 GTCAAC GT[CT]^[GA]AC 740 GTCAAC KpnI 61 GGTACC GGTAC^C 3483 GGTACC NciI 994 CCGGG CC^[GC]GG 2953 CCGGG SbfI 91 CCTGCAGG CCTGCA^GG 1114 CCTGCAGG Sse8387I 91 CCTGCAGG CCTGCA^GG 1114 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I TspRI Van91I XcmI XhoII