##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AccIII      AclI        AcsI        AgeI        Aor51HI     
ApoI        AscI        AseI        AsnI        AviII       
AvrII       BbeI        BcgI        BglII       BlnI        
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     ClaI        CpoI        DraI        EagI        
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
FspI        HpaI        KasI        KspI        MfeI        
MroI        MunI        NarI        NdeI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Tsp509I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         419  GACGTC               GACGT^C
AflII        3598  CTTAAG               C^TTAAG
AhdI          512  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3182  GTGCAC               G^TGCAC
ApaLI        3182  GTGCAC               G^TGCAC
Asp700I      1342  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         512  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1729  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          317  CTCGGG               C^[CT]CG[GA]G
BamHI        2235  GGATCC               G^GATCC
BbrPI        1009  CACGTG               CAC^GTG
BbvCI        2416  CCTCAGC              CCTCAGC(-5/-2)
BclI         2004  TGATCA               T^GATCA
BfrI         3598  CTTAAG               C^TTAAG
BlpI         2763  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2763  GCTGAGC              GC^T[ACGT]AGC
BsaBI         534  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1836  TCCGGT               [AT]^CCGG[AT]
BsmI         2743  GAATGC               GAATGC(1/-1)
BsoBI         317  CTCGGG               C^[CT]CG[GA]G
BspHI        1752  TCATGA               T^CATGA
CelII        2763  GCTGAGC              GC^T[ACGT]AGC
DraIII       2177  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      512  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      2082  ATGCAT               ATGCA^T
FbaI         2004  TGATCA               T^GATCA
FseI         3976  GGCCGGCC             GGCCGG^CC
HaeII        2633  AGCGCC               [GA]GCGC^[CT]
HindIII      2864  AAGCTT               A^AGCTT
MamI          534  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          999  ACGCGT               A^CGCGT
NaeI         3977  GCCGGC               GCC^GGC
NgoMIV       3977  GCCGGC               G^CCGGC
NheI         3241  GCTAGC               G^CTAGC
NsiI         2082  ATGCAT               ATGCA^T
NspI         2481  ACATGC               [GA]CATG^[CT]
PflFI        1729  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1009  CACGTG               CAC^GTG
PmlI         1009  CACGTG               CAC^GTG
PshAI        1759  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1752  TCATGA               T^CATGA
SacI         1415  GAGCTC               GAGCT^C
ScaI         3383  AGTACT               AGT^ACT
SexAI         921  ACCTGGT              A^CC[AT]GGT
SfiI         3767  GGCCTGGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI          542  TCGA                 T^CGA
Tth111I      1729  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       542  TCGA                 T^CGA
XbaI         1822  TCTAGA               T^CTAGA
XmnI         1342  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Acc65I         61  GGTACC               G^GTACC
             3483  GGTACC              
AflIII        999  ACGCGT               A^C[GA][CT]GT
             1010  ACGTGT              
ApaI          201  GGGCCC               GGGCC^C
             4001  GGGCCC              
Asp718I        61  GGTACC               G^GTACC
             3483  GGTACC              
BciVI        1003  GTATCC               GTATCC(6/5)
             3196  GTATCC              
BcnI          994  CCGGG                CC^[GC]GG
             2953  CCGGG               
BglI         2012  GCCAAGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3768  GCCTGGGGGGC         
BsaAI        1009  CACGTG               [CT]AC^GT[GA]
             2504  TACGTG              
BsrDI         490  CATTGC               GCAATG(2/0)
             1950  GCAATG              
BstEII        382  GGTCACC              G^GT[ACGT]ACC
             3255  GGTTACC             
BstPI         382  GGTCACC              G^GT[ACGT]ACC
             3255  GGTTACC             
BstXI        1872  CCATCAGGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3898  CCATTGGTCTGG        
EcoO65I       382  GGTCACC              G^GT[ACGT]ACC
             3255  GGTTACC             
HincII        558  GTCAAC               GT[CT]^[GA]AC
              740  GTCAAC              
HindII        558  GTCAAC               GT[CT]^[GA]AC
              740  GTCAAC              
KpnI           61  GGTACC               GGTAC^C
             3483  GGTACC              
NciI          994  CCGGG                CC^[GC]GG
             2953  CCGGG               
SbfI           91  CCTGCAGG             CCTGCA^GG
             1114  CCTGCAGG            
Sse8387I       91  CCTGCAGG             CCTGCA^GG
             1114  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TfiI        Tru9I       TseI        Tsp45I      TspRI       
Van91I      XcmI        XhoII