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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BcgI BglI
BglII BlnI BlpI Bpu1102I BsaI
BsaWI BseAI BsiWI BsoBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HpaI
KasI KpnI KspI MflI MluI
MroI NaeI NarI NcoI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI Tth111I XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2935 GTAGAC GT^[AC][GT]AC
AcyI 22 GGCGTC G[GA]^CG[CT]C
Alw44I 538 GTGCAC G^TGCAC
AlwNI 869 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 702 CCTGAGG CC^T[ACGT]AGG
ApaI 2956 GGGCCC GGGCC^C
ApaLI 538 GTGCAC G^TGCAC
AvaII 1814 GGTCC G^G[AT]CC
BbvCI 1351 GCTGAGG CCTCAGC(-5/-2)
BciVI 2993 GGATAC GTATCC(6/5)
BclI 1311 TGATCA T^GATCA
BcnI 101 CCCGG CC^[GC]GG
BsaHI 22 GGCGTC G[GA]^CG[CT]C
BsmBI 2 GAGACG CGTCTC(1/5)
BstBI 1775 TTCGAA TT^CGAA
BstXI 2261 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 702 CCTGAGG CC^T[ACGT]AGG
DsaI 235 CCACGG C^C[GA][CT]GG
Eco81I 702 CCTGAGG CC^T[ACGT]AGG
EcoT22I 391 ATGCAT ATGCA^T
FbaI 1311 TGATCA T^GATCA
HapII 102 CCGG C^CGG
HgaI 23 GCGTC GACGC(5/10)
Hin1I 22 GGCGTC G[GA]^CG[CT]C
HpaII 102 CCGG C^CGG
MspI 102 CCGG C^CGG
NciI 101 CCCGG CC^[GC]GG
NheI 777 GCTAGC G^CTAGC
NsiI 391 ATGCAT ATGCA^T
NspV 1775 TTCGAA TT^CGAA
PflMI 235 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2135 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1602 GAGCTC GAGCT^C
SapI 1270 GAAGAGC GCTCTTC(1/4)
SfuI 1775 TTCGAA TT^CGAA
SwaI 3001 ATTTAAAT ATTT^AAAT
Tsp45I 47 GTGAC ^GT[GC]AC
Van91I 235 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 734 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3579 GACTTGGTGTC
AspEI 734 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3579 GACTTGGTGTC
BbrPI 41 CACGTG CAC^GTG
1406 CACGTG
BpmI 2285 CTGGAG CTGGAG(16/14)
3146 CTGGAG
BsaAI 41 CACGTG [CT]AC^GT[GA]
1406 CACGTG
BsaBI 820 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
859 GATCTCAATC
BsmFI 791 GTCCC GGGAC(10/14)
2839 GTCCC
Bsp1407I 2171 TGTACA T^GTACA
2705 TGTACA
BsrBI 266 CCGCTC CCGCTC(-3/-3)
71 GAGCGG
BsrGI 2171 TGTACA T^GTACA
2705 TGTACA
BstNI 1542 CCTGG CC^[AT]GG
2243 CCTGG
DraII 2955 AGGGCCC [GA]G^G[ACGT]CC[CT]
2956 GGGCCCT
DraIII 41 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1403 CACCACGTG
Eam1105I 734 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3579 GACTTGGTGTC
EcoO109I 2955 AGGGCCC [GA]G^G[ACGT]CC[CT]
2956 GGGCCCT
EcoRII 1542 CCTGG ^CC[AT]GG
2243 CCTGG
HaeII 211 GGCGCT [GA]GCGC^[CT]
291 GGCGCT
MamI 820 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
859 GATCTCAATC
MfeI 3123 CAATTG C^AATTG
3469 CAATTG
MspA1I 1026 CAGCTG C[AC]G^C[GT]G
1307 CAGCTG
MunI 3123 CAATTG C^AATTG
3469 CAATTG
MvaI 1542 CCTGG CC^[AT]GG
2243 CCTGG
NdeI 2048 CATATG CA^TATG
3554 CATATG
PmaCI 41 CACGTG CAC^GTG
1406 CACGTG
PmlI 41 CACGTG CAC^GTG
1406 CACGTG
PvuII 1026 CAGCTG CAG^CTG
1307 CAGCTG
ScaI 1615 AGTACT AGT^ACT
3266 AGTACT
SspBI 2171 TGTACA T^GTACA
2705 TGTACA
XbaI 345 TCTAGA T^CTAGA
1009 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI ApoI Asp700I AspHI
BalI BanII BbsI BbvI BfaI
BfrI BmyI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI XmnI