##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

Acc65I      AclI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       BalI        BbeI        BcgI        
BglII       BsiWI       BspLU11I    BssHII      Bst1107I    
BstBI       BstZ17I     CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoT22I     FseI        
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        1859  GACGTC               GACGT^C
AflII        4437  CTTAAG               C^TTAAG
AflIII       2014  ACACGT               A^C[GA][CT]GT
AgeI         2779  ACCGGT               A^CCGGT
AvrII        2135  CCTAGG               C^CTAGG
BamHI        1397  GGATCC               G^GATCC
BbrPI        3570  CACGTG               CAC^GTG
BfrI         4437  CTTAAG               C^TTAAG
BlnI         2135  CCTAGG               C^CTAGG
BlpI          346  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      346  GCTCAGC              GC^T[ACGT]AGC
BsiEI        3018  CGGTCG               CG[GA][CT]^CG
BspDI         247  ATCGAT               AT^CGAT
BsrDI        2502  GCAATG               GCAATG(2/0)
BssSI        3891  CACGAG               CACGAG(-5/-1)
BstEII        406  GGTAACC              G^GT[ACGT]ACC
BstPI         406  GGTAACC              G^GT[ACGT]ACC
CelII         346  GCTCAGC              GC^T[ACGT]AGC
ClaI          247  ATCGAT               AT^CGAT
DraIII       1956  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1438  CGGCCA               [CT]^GGCC[GA]
EcoO65I       406  GGTAACC              G^GT[ACGT]ACC
HaeII        1888  GGCGCT               [GA]GCGC^[CT]
HincII       2292  GTCAAC               GT[CT]^[GA]AC
HindII       2292  GTCAAC               GT[CT]^[GA]AC
HindIII       220  AAGCTT               A^AGCTT
NcoI         1949  CCATGG               C^CATGG
NdeI         1053  CATATG               CA^TATG
PflMI         699  CCAAATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2779  ACCGGT               A^CCGGT
PmaCI        3570  CACGTG               CAC^GTG
PmlI         3570  CACGTG               CAC^GTG
SexAI        2431  ACCTGGT              A^CC[AT]GGT
SfiI         3806  GGCCCAGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI         4099  AGGCCT               AGG^CCT
SwaI          807  ATTTAAAT             ATTT^AAAT
Van91I        699  CCAAATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AccI         1457  GTCTAC               GT^[AC][GT]AC
             3001  GTAGAC              
AccIII       3746  TCCGGA               T^CCGGA
             3922  TCCGGA              
AcyI         1859  GACGTC               G[GA]^CG[CT]C
             4239  GACGCC              
AocI         2265  CCTAAGG              CC^T[ACGT]AGG
             3189  CCTGAGG             
ApaI         2369  GGGCCC               GGGCC^C
             3797  GGGCCC              
AspI          744  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3344  GACAGAGTC           
BbvCI        3006  CCTCAGC              CCTCAGC(-5/-2)
             4166  GCTGAGG             
BciVI         385  GTATCC               GTATCC(6/5)
              483  GTATCC              
BglI         1880  GCCAGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3807  GCCCAGCAGGC         
BsaBI        1934  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1940  GATCTTCATC          
BsaHI        1859  GACGTC               G[GA]^CG[CT]C
             4239  GACGCC              
BseAI        3746  TCCGGA               T^CCGGA
             3922  TCCGGA              
BsmBI        1857  GAGACG               CGTCTC(1/5)
             4143  GAGACG              
BsmI         1628  GAATGC               GAATGC(1/-1)
             3846  GAATGC              
Bsp1407I     1990  TGTACA               T^GTACA
             2005  TGTACA              
BspEI        3746  TCCGGA               T^CCGGA
             3922  TCCGGA              
BspHI         997  TCATGA               T^CATGA
             1580  TCATGA              
BsrBI        2930  CCGCTC               CCGCTC(-3/-3)
             1884  GAGCGG              
BsrFI        2485  ACCGGC               [GA]^CCGG[CT]
             2779  ACCGGT              
BsrGI        1990  TGTACA               T^GTACA
             2005  TGTACA              
Bsu36I       2265  CCTAAGG              CC^T[ACGT]AGG
             3189  CCTGAGG             
Cfr10I       2485  ACCGGC               [GA]^CCGG[CT]
             2779  ACCGGT              
DrdI         3004  GACCTCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3903  GACCTGCTTGTC        
DsaI         1949  CCATGG               C^C[GA][CT]GG
             3619  CCGTGG              
Eco81I       2265  CCTAAGG              CC^T[ACGT]AGG
             3189  CCTGAGG             
EcoNI        4123  CCTCCGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4190  CCTGCAAAAGG         
EcoRI        1257  GAATTC               G^AATTC
             3989  GAATTC              
Hin1I        1859  GACGTC               G[GA]^CG[CT]C
             4239  GACGCC              
MamI         1934  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1940  GATCTTCATC          
MroI         3746  TCCGGA               T^CCGGA
             3922  TCCGGA              
PflFI         744  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3344  GACAGAGTC           
PpuMI        1345  AGGACCT              [GA]G^G[AT]CC[CT]
             4252  AGGACCT             
PstI         4158  CTGCAG               CTGCA^G
             4328  CTGCAG              
RcaI          997  TCATGA               T^CATGA
             1580  TCATGA              
ScaI         1699  AGTACT               AGT^ACT
             4024  AGTACT              
SspBI        1990  TGTACA               T^GTACA
             2005  TGTACA              
Tth111I       744  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3344  GACAGAGTC           
XcmI         1614  CCACTGCCCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3687  CCAAGAGACCGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       AviII       BanI        BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       EarI        EcoO109I    EcoRII      EcoRV       
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
FspI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmaCI       XmaI