################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AclI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI BalI BbeI BcgI BglII BsiWI BspLU11I BssHII Bst1107I BstBI BstZ17I CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 1859 GACGTC GACGT^C AflII 4437 CTTAAG C^TTAAG AflIII 2014 ACACGT A^C[GA][CT]GT AgeI 2779 ACCGGT A^CCGGT AvrII 2135 CCTAGG C^CTAGG BamHI 1397 GGATCC G^GATCC BbrPI 3570 CACGTG CAC^GTG BfrI 4437 CTTAAG C^TTAAG BlnI 2135 CCTAGG C^CTAGG BlpI 346 GCTCAGC GC^T[ACGT]AGC Bpu1102I 346 GCTCAGC GC^T[ACGT]AGC BsiEI 3018 CGGTCG CG[GA][CT]^CG BspDI 247 ATCGAT AT^CGAT BsrDI 2502 GCAATG GCAATG(2/0) BssSI 3891 CACGAG CACGAG(-5/-1) BstEII 406 GGTAACC G^GT[ACGT]ACC BstPI 406 GGTAACC G^GT[ACGT]ACC CelII 346 GCTCAGC GC^T[ACGT]AGC ClaI 247 ATCGAT AT^CGAT DraIII 1956 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1438 CGGCCA [CT]^GGCC[GA] EcoO65I 406 GGTAACC G^GT[ACGT]ACC HaeII 1888 GGCGCT [GA]GCGC^[CT] HincII 2292 GTCAAC GT[CT]^[GA]AC HindII 2292 GTCAAC GT[CT]^[GA]AC HindIII 220 AAGCTT A^AGCTT NcoI 1949 CCATGG C^CATGG NdeI 1053 CATATG CA^TATG PflMI 699 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2779 ACCGGT A^CCGGT PmaCI 3570 CACGTG CAC^GTG PmlI 3570 CACGTG CAC^GTG SexAI 2431 ACCTGGT A^CC[AT]GGT SfiI 3806 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 4099 AGGCCT AGG^CCT SwaI 807 ATTTAAAT ATTT^AAAT Van91I 699 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccI 1457 GTCTAC GT^[AC][GT]AC 3001 GTAGAC AccIII 3746 TCCGGA T^CCGGA 3922 TCCGGA AcyI 1859 GACGTC G[GA]^CG[CT]C 4239 GACGCC AocI 2265 CCTAAGG CC^T[ACGT]AGG 3189 CCTGAGG ApaI 2369 GGGCCC GGGCC^C 3797 GGGCCC AspI 744 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3344 GACAGAGTC BbvCI 3006 CCTCAGC CCTCAGC(-5/-2) 4166 GCTGAGG BciVI 385 GTATCC GTATCC(6/5) 483 GTATCC BglI 1880 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3807 GCCCAGCAGGC BsaBI 1934 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1940 GATCTTCATC BsaHI 1859 GACGTC G[GA]^CG[CT]C 4239 GACGCC BseAI 3746 TCCGGA T^CCGGA 3922 TCCGGA BsmBI 1857 GAGACG CGTCTC(1/5) 4143 GAGACG BsmI 1628 GAATGC GAATGC(1/-1) 3846 GAATGC Bsp1407I 1990 TGTACA T^GTACA 2005 TGTACA BspEI 3746 TCCGGA T^CCGGA 3922 TCCGGA BspHI 997 TCATGA T^CATGA 1580 TCATGA BsrBI 2930 CCGCTC CCGCTC(-3/-3) 1884 GAGCGG BsrFI 2485 ACCGGC [GA]^CCGG[CT] 2779 ACCGGT BsrGI 1990 TGTACA T^GTACA 2005 TGTACA Bsu36I 2265 CCTAAGG CC^T[ACGT]AGG 3189 CCTGAGG Cfr10I 2485 ACCGGC [GA]^CCGG[CT] 2779 ACCGGT DrdI 3004 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3903 GACCTGCTTGTC DsaI 1949 CCATGG C^C[GA][CT]GG 3619 CCGTGG Eco81I 2265 CCTAAGG CC^T[ACGT]AGG 3189 CCTGAGG EcoNI 4123 CCTCCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4190 CCTGCAAAAGG EcoRI 1257 GAATTC G^AATTC 3989 GAATTC Hin1I 1859 GACGTC G[GA]^CG[CT]C 4239 GACGCC MamI 1934 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1940 GATCTTCATC MroI 3746 TCCGGA T^CCGGA 3922 TCCGGA PflFI 744 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3344 GACAGAGTC PpuMI 1345 AGGACCT [GA]G^G[AT]CC[CT] 4252 AGGACCT PstI 4158 CTGCAG CTGCA^G 4328 CTGCAG RcaI 997 TCATGA T^CATGA 1580 TCATGA ScaI 1699 AGTACT AGT^ACT 4024 AGTACT SspBI 1990 TGTACA T^GTACA 2005 TGTACA Tth111I 744 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3344 GACAGAGTC XcmI 1614 CCACTGCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3687 CCAAGAGACCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII AviII BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI