##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI Asp700I AviII BclI BfrI
BglII BseAI BsiWI BspEI BspHI
BssHII BssSI BstBI BstEII BstPI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FspI
KspI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RcaI SacII
SalI SbfI SfuI SmaI SnaBI
Sse8387I SspI SwaI XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 4058 GACGTC GACGT^C
AflIII 2059 ACATGT A^C[GA][CT]GT
AhdI 2512 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3734 ATTAAT AT^TAAT
AsnI 3734 ATTAAT AT^TAAT
AspEI 2512 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3139 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2623 TGGCCA TGG^CCA
BamHI 1478 GGATCC G^GATCC
BcgI 1386 CGAGGATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3429 GTATCC GTATCC(6/5)
BsaBI 3642 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1571 ACCGGA [AT]^CCGG[AT]
BsiEI 1569 CGACCG CG[GA][CT]^CG
BsmBI 4017 CGTCTC CGTCTC(1/5)
BsmI 2074 GAATGC GAATGC(1/-1)
Bsp1407I 3306 TGTACA T^GTACA
BspDI 2872 ATCGAT AT^CGAT
BspLU11I 2059 ACATGT A^CATGT
BsrBI 2200 GAGCGG CCGCTC(-3/-3)
BsrGI 3306 TGTACA T^GTACA
Bst1107I 1747 GTATAC GTA^TAC
BstZ17I 1747 GTATAC GTA^TAC
ClaI 2872 ATCGAT AT^CGAT
CpoI 377 CGGACCG CG^G[AT]CCG
DraI 2994 TTTAAA TTT^AAA
DraIII 2432 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2512 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 3989 GGCCGGCC GGCCGG^CC
HincII 3822 GTTAAC GT[CT]^[GA]AC
HindII 3822 GTTAAC GT[CT]^[GA]AC
HindIII 3077 AAGCTT A^AGCTT
HpaI 3822 GTTAAC GTT^AAC
MamI 3642 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2623 TGGCCA TGG^CCA
MscI 2623 TGGCCA TGG^CCA
NcoI 1434 CCATGG C^CATGG
PflFI 3139 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3734 ATTAAT AT^TAAT
RsrII 377 CGGACCG CG^G[AT]CCG
SapI 1713 GCTCTTC GCTCTTC(1/4)
ScaI 287 AGTACT AGT^ACT
SfiI 3989 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1260 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2956 GCATGC GCATG^C
SspBI 3306 TGTACA T^GTACA
Tth111I 3139 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2 GGTACC G^GTACC
3800 GGTACC
AcsI 543 GAATTC [GA]^AATT[CT]
4098 GAATTC
Alw44I 2687 GTGCAC G^TGCAC
3019 GTGCAC
ApaLI 2687 GTGCAC G^TGCAC
3019 GTGCAC
ApoI 543 GAATTC [GA]^AATT[CT]
4098 GAATTC
Asp718I 2 GGTACC G^GTACC
3800 GGTACC
AvrII 2727 CCTAGG C^CTAGG
3618 CCTAGG
BbeI 522 GGCGCC GGCGC^C
1264 GGCGCC
BbrPI 1483 CACGTG CAC^GTG
2532 CACGTG
BbsI 91 GAAGAC GAAGAC(2/6)
2343 GAAGAC
BglI 2021 GCCCTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3990 GCCGGCCTGGC
BlnI 2727 CCTAGG C^CTAGG
3618 CCTAGG
BlpI 567 GCTCAGC GC^T[ACGT]AGC
1280 GCTCAGC
Bpu1102I 567 GCTCAGC GC^T[ACGT]AGC
1280 GCTCAGC
BpuAI 91 GAAGAC GAAGAC(2/6)
2343 GAAGAC
BsaAI 1483 CACGTG [CT]AC^GT[GA]
2532 CACGTG
BstXI 2600 CCAAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3937 CCAGCACTTTGG
CelII 567 GCTCAGC GC^T[ACGT]AGC
1280 GCTCAGC
EcoRI 543 GAATTC G^AATTC
4098 GAATTC
HaeII 522 GGCGCC [GA]GCGC^[CT]
1264 GGCGCC
KasI 522 GGCGCC G^GCGCC
1264 GGCGCC
KpnI 2 GGTACC GGTAC^C
3800 GGTACC
NaeI 1261 GCCGGC GCC^GGC
3990 GCCGGC
NarI 522 GGCGCC GG^CGCC
1264 GGCGCC
NgoMIV 1261 GCCGGC G^CCGGC
3990 GCCGGC
PmaCI 1483 CACGTG CAC^GTG
2532 CACGTG
PmlI 1483 CACGTG CAC^GTG
2532 CACGTG
SexAI 2433 ACCTGGT A^CC[AT]GGT
2656 ACCTGGT
SfoI 522 GGCGCC GGC^GCC
1264 GGCGCC
SpeI 1697 ACTAGT A^CTAGT
3843 ACTAGT
XcmI 1406 CCAGCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3402 CCAGAAACACATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII