##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BamHI       
BbrPI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsiEI       BsiWI       Bsp1407I    
BspHI       BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       CpoI        
DraIII      EagI        EclXI       Eco52I      EcoNI       
EcoO65I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KpnI        KspI        MfeI        
MluI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SspBI       SwaI        
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        577  TCCGGA               T^CCGGA
AcyI          162  GGCGCC               G[GA]^CG[CT]C
AflIII        651  ACATGT               A^C[GA][CT]GT
AhdI         3268  GACCTATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2389  GTGCAC               G^TGCAC
Aor51HI      1035  AGCGCT               AGC^GCT
ApaLI        2389  GTGCAC               G^TGCAC
AspEI        3268  GACCTATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          162  GGCGCC               GGCGC^C
BcgI         3220  CGAGTGTTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2722  GGATAC               GTATCC(6/5)
BglI         1859  GCCAGTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        2099  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         162  GGCGCC               G[GA]^CG[CT]C
BsaWI         577  TCCGGA               [AT]^CCGG[AT]
BseAI         577  TCCGGA               T^CCGGA
BsmI         2183  GAATGC               GAATGC(1/-1)
BspDI        3981  ATCGAT               AT^CGAT
BspEI         577  TCCGGA               T^CCGGA
BspLU11I      651  ACATGT               A^CATGT
BsrBI        2691  GAGCGG               CCGCTC(-3/-3)
BsrDI         980  CATTGC               GCAATG(2/0)
ClaI         3981  ATCGAT               AT^CGAT
DraI         3234  TTTAAA               TTT^AAA
DrdI         1658  GACAGCTCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           86  CCACGG               C^C[GA][CT]GG
Eam1105I     3268  GACCTATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1035  AGCGCT               AGC^GCT
EcoRI        1799  GAATTC               G^AATTC
EcoT14I      3010  CCAAGG               C^C[AT][AT]GG
Hin1I         162  GGCGCC               G[GA]^CG[CT]C
KasI          162  GGCGCC               G^GCGCC
MamI         2099  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          577  TCCGGA               T^CCGGA
NarI          162  GGCGCC               GG^CGCC
PacI          729  TTAATTAA             TTAAT^TAA
PshAI        1178  GACTCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2286  GAGCTC               GAGCT^C
SfoI          162  GGCGCC               GGC^GCC
SphI         2158  GCATGC               GCATG^C
StyI         3010  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AocI         2766  CCTCAGG              CC^T[ACGT]AGG
             4073  CCTGAGG             
BalI         1163  TGGCCA               TGG^CCA
             1619  TGGCCA              
BanII        1448  GAGCCC               G[GA]GC[CT]^C
             2286  GAGCTC              
BsmBI         144  GAGACG               CGTCTC(1/5)
             4033  GAGACG              
BssHII         25  GCGCGC               G^CGCGC
               27  GCGCGC              
BstAPI       2382  GCAGCGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2929  GCATCCACTGC         
Bsu36I       2766  CCTCAGG              CC^T[ACGT]AGG
             4073  CCTGAGG             
EarI         3289  CTCTTC               CTCTTC(1/4)
             2460  GAAGAG              
Eco81I       2766  CCTCAGG              CC^T[ACGT]AGG
             4073  CCTGAGG             
EcoRV         385  GATATC               GAT^ATC
             2526  GATATC              
EcoT22I      2484  ATGCAT               ATGCA^T
             3090  ATGCAT              
HaeII         162  GGCGCC               [GA]GCGC^[CT]
             1035  AGCGCT              
HindIII       921  AAGCTT               A^AGCTT
             2682  AAGCTT              
Ksp632I      3289  CTCTTC               CTCTTC(1/4)
             2460  GAAGAG              
MaeII        2302  ACGT                 A^CGT
             4036  ACGT                
MluNI        1163  TGGCCA               TGG^CCA
             1619  TGGCCA              
MscI         1163  TGGCCA               TGG^CCA
             1619  TGGCCA              
NsiI         2484  ATGCAT               ATGCA^T
             3090  ATGCAT              
SapI         2460  GAAGAGC              GCTCTTC(1/4)
             3288  GCTCTTC             
SmaI         2784  CCCGGG               CCC^GGG
             3200  CCCGGG              
SmlI         1304  CTTGAG               C^T[CT][GA]AG
             2364  CTTGAG              
SpeI         1344  ACTAGT               A^CTAGT
             3871  ACTAGT              
SspI          603  AATATT               AAT^ATT
              826  AATATT              
XcmI         1121  CCAGGGTGCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2394  CCATTTCTAAAGTGG     
XmaCI        2784  CCCGGG               C^CCGGG
             3200  CCCGGG              
XmaI         2784  CCCGGG               C^CCGGG
             3200  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
SbfI        ScrFI       SexAI       SfaNI       SfcI        
Sse8387I    StuI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII