##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI BamHI BcgI BciVI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NsiI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfiI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1766 GTAGAC GT^[AC][GT]AC
AccIII 4043 TCCGGA T^CCGGA
AcsI 584 AAATTT [GA]^AATT[CT]
AcyI 3510 GGCGCC G[GA]^CG[CT]C
AflII 3251 CTTAAG C^TTAAG
AflIII 1062 ACACGT A^C[GA][CT]GT
AgeI 990 ACCGGT A^CCGGT
Aor51HI 4267 AGCGCT AGC^GCT
ApoI 584 AAATTT [GA]^AATT[CT]
AviII 210 TGCGCA TGC^GCA
AvrII 1400 CCTAGG C^CTAGG
BbeI 3510 GGCGCC GGCGC^C
BclI 1656 TGATCA T^GATCA
BfrI 3251 CTTAAG C^TTAAG
BlnI 1400 CCTAGG C^CTAGG
BsaHI 3510 GGCGCC G[GA]^CG[CT]C
BseAI 4043 TCCGGA T^CCGGA
BsmBI 1875 GAGACG CGTCTC(1/5)
BspEI 4043 TCCGGA T^CCGGA
DrdI 1331 GACTTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4267 AGCGCT AGC^GCT
FbaI 1656 TGATCA T^GATCA
FseI 1497 GGCCGGCC GGCCGG^CC
FspI 210 TGCGCA TGC^GCA
Hin1I 3510 GGCGCC G[GA]^CG[CT]C
HpaI 4330 GTTAAC GTT^AAC
KasI 3510 GGCGCC G^GCGCC
MroI 4043 TCCGGA T^CCGGA
NaeI 1498 GCCGGC GCC^GGC
NarI 3510 GGCGCC GG^CGCC
NcoI 1510 CCATGG C^CATGG
NgoMIV 1498 GCCGGC G^CCGGC
NruI 3942 TCGCGA TCG^CGA
PaeR7I 719 CTCGAG C^TCGAG
PinAI 990 ACCGGT A^CCGGT
SbfI 2989 CCTGCAGG CCTGCA^GG
ScaI 3570 AGTACT AGT^ACT
SexAI 874 ACCTGGT A^CC[AT]GGT
SfoI 3510 GGCGCC GGC^GCC
SphI 405 GCATGC GCATG^C
Sse8387I 2989 CCTGCAGG CCTGCA^GG
XcmI 865 CCAAGGGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 719 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 2965 GATCC GGATC(4/5)
4098 GATCC
BbrPI 227 CACGTG CAC^GTG
3259 CACGTG
BglII 1391 AGATCT A^GATCT
3313 AGATCT
BsrDI 36 CATTGC GCAATG(2/0)
3281 GCAATG
BsrFI 990 ACCGGT [GA]^CCGG[CT]
1498 GCCGGC
BssSI 195 CACGAG CACGAG(-5/-1)
2969 CACGAG
BstEII 993 GGTCACC G^GT[ACGT]ACC
3762 GGTCACC
BstPI 993 GGTCACC G^GT[ACGT]ACC
3762 GGTCACC
BstXI 2140 CCATGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2239 CCAGAGCCCTGG
Cfr10I 990 ACCGGT [GA]^CCGG[CT]
1498 GCCGGC
EcoO65I 993 GGTCACC G^GT[ACGT]ACC
3762 GGTCACC
HindIII 2372 AAGCTT A^AGCTT
4417 AAGCTT
PmaCI 227 CACGTG CAC^GTG
3259 CACGTG
PmlI 227 CACGTG CAC^GTG
3259 CACGTG
SmaI 2547 CCCGGG CCC^GGG
2583 CCCGGG
XmaCI 2547 CCCGGG C^CCGGG
2583 CCCGGG
XmaI 2547 CCCGGG C^CCGGG
2583 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwNI AocI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII