##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BamHI BbrPI
BbvCI BfrI BglII BlpI Bpu1102I
BsaAI BsiWI BsmBI Bsp1407I BsrGI
Bst1107I BstXI BstZ17I CelII CpoI
Eam1105I EcoRI EcoRV FseI HpaI
KpnI KspI MfeI MluI MunI
NheI NotI PacI PaeR7I PflFI
PinAI PmaCI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 676 GACGTC GACGT^C
AccIII 2906 TCCGGA T^CCGGA
Alw44I 3853 GTGCAC G^TGCAC
AocI 1368 CCTCAGG CC^T[ACGT]AGG
ApaLI 3853 GTGCAC G^TGCAC
AviII 501 TGCGCA TGC^GCA
AvrII 1837 CCTAGG C^CTAGG
BbeI 3202 GGCGCC GGCGC^C
BcgI 1399 GCACGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1775 GGATAC GTATCC(6/5)
BclI 4179 TGATCA T^GATCA
BlnI 1837 CCTAGG C^CTAGG
BsaBI 3410 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2906 TCCGGA T^CCGGA
BsiEI 9 CGGCCG CG[GA][CT]^CG
BspEI 2906 TCCGGA T^CCGGA
BsrBI 2836 CCGCTC CCGCTC(-3/-3)
BssHII 40 GCGCGC G^CGCGC
BstAPI 3092 GCAACAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1470 TTCGAA TT^CGAA
Bsu36I 1368 CCTCAGG CC^T[ACGT]AGG
DraI 769 TTTAAA TTT^AAA
DraIII 2137 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 9 CGGCCG C^GGCCG
EarI 3458 CTCTTC CTCTTC(1/4)
EclXI 9 CGGCCG C^GGCCG
Eco52I 9 CGGCCG C^GGCCG
Eco81I 1368 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1582 ATGCAT ATGCA^T
FbaI 4179 TGATCA T^GATCA
FspI 501 TGCGCA TGC^GCA
HindIII 3274 AAGCTT A^AGCTT
KasI 3202 GGCGCC G^GCGCC
Ksp632I 3458 CTCTTC CTCTTC(1/4)
MamI 3410 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2906 TCCGGA T^CCGGA
NarI 3202 GGCGCC GG^CGCC
NcoI 2028 CCATGG C^CATGG
NdeI 3009 CATATG CA^TATG
NruI 1656 TCGCGA TCG^CGA
NsiI 1582 ATGCAT ATGCA^T
NspV 1470 TTCGAA TT^CGAA
PflMI 2329 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 768 GTTTAAAC GTTT^AAAC
PvuII 82 CAGCTG CAG^CTG
SacI 2774 GAGCTC GAGCT^C
SfoI 3202 GGCGCC GGC^GCC
SfuI 1470 TTCGAA TT^CGAA
Van91I 2329 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3122 GTAGAC GT^[AC][GT]AC
3329 GTAGAC
AflIII 1674 ACATGT A^C[GA][CT]GT
3879 ACATGT
Aor51HI 58 AGCGCT AGC^GCT
2483 AGCGCT
AvaI 2914 CTCGGG C^[CT]CG[GA]G
3306 CCCGAG
BanII 1094 GAGCCC G[GA]GC[CT]^C
2774 GAGCTC
BsoBI 2914 CTCGGG C^[CT]CG[GA]G
3306 CCCGAG
BspDI 1358 ATCGAT AT^CGAT
1445 ATCGAT
BspHI 1948 TCATGA T^CATGA
3957 TCATGA
BspLU11I 1674 ACATGT A^CATGT
3879 ACATGT
BssSI 1400 CACGAG CACGAG(-5/-1)
3383 CACGAG
BstEII 352 GGTGACC G^GT[ACGT]ACC
2640 GGTCACC
BstPI 352 GGTGACC G^GT[ACGT]ACC
2640 GGTCACC
ClaI 1358 ATCGAT AT^CGAT
1445 ATCGAT
DrdI 452 GACGGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1936 GACCATAGTGTC
DsaI 2028 CCATGG C^C[GA][CT]GG
2977 CCACGG
Eco47III 58 AGCGCT AGC^GCT
2483 AGCGCT
EcoNI 1414 CCTAGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3160 CCTGGCACAGG
EcoO65I 352 GGTGACC G^GT[ACGT]ACC
2640 GGTCACC
NaeI 7 GCCGGC GCC^GGC
44 GCCGGC
NgoMIV 7 GCCGGC G^CCGGC
44 GCCGGC
PpuMI 1200 AGGACCT [GA]G^G[AT]CC[CT]
2383 AGGTCCT
PstI 91 CTGCAG CTGCA^G
2682 CTGCAG
RcaI 1948 TCATGA T^CATGA
3957 TCATGA
SphI 909 GCATGC GCATG^C
1584 GCATGC
SspI 3320 AATATT AAT^ATT
4165 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII