##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AscI Asp700I AspI AvrII
BamHI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsiWI BspDI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI EcoRV EcoT22I
FbaI HincII HindII HpaI MamI
MfeI MluI MunI NdeI NotI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SalI SbfI ScaI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1081 GGTACC G^GTACC
AcsI 1767 AAATTT [GA]^AATT[CT]
ApoI 1767 AAATTT [GA]^AATT[CT]
AseI 3156 ATTAAT AT^TAAT
AsnI 3156 ATTAAT AT^TAAT
Asp718I 1081 GGTACC G^GTACC
AviII 4040 TGCGCA TGC^GCA
BbrPI 2778 CACGTG CAC^GTG
BbsI 4217 GAAGAC GAAGAC(2/6)
BpuAI 4217 GAAGAC GAAGAC(2/6)
BsaAI 2778 CACGTG [CT]AC^GT[GA]
BsmBI 4137 GAGACG CGTCTC(1/5)
Bsp1407I 3484 TGTACA T^GTACA
BspHI 1843 TCATGA T^CATGA
BsrGI 3484 TGTACA T^GTACA
BssHII 189 GCGCGC G^CGCGC
BstEII 1718 GGTGACC G^GT[ACGT]ACC
BstPI 1718 GGTGACC G^GT[ACGT]ACC
DraI 2765 TTTAAA TTT^AAA
EcoO65I 1718 GGTGACC G^GT[ACGT]ACC
FseI 3707 GGCCGGCC GGCCGG^CC
FspI 4040 TGCGCA TGC^GCA
HindIII 3933 AAGCTT A^AGCTT
KpnI 1081 GGTACC GGTAC^C
KspI 708 CCGCGG CCGC^GG
NheI 32 GCTAGC G^CTAGC
NruI 4228 TCGCGA TCG^CGA
PmaCI 2778 CACGTG CAC^GTG
PmlI 2778 CACGTG CAC^GTG
PshAI 303 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3156 ATTAAT AT^TAAT
RcaI 1843 TCATGA T^CATGA
SacII 708 CCGCGG CCGC^GG
SspBI 3484 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 3772 TCCGGA T^CCGGA
4258 TCCGGA
Alw44I 804 GTGCAC G^TGCAC
4203 GTGCAC
AocI 1800 CCTGAGG CC^T[ACGT]AGG
3602 CCTGAGG
ApaLI 804 GTGCAC G^TGCAC
4203 GTGCAC
BalI 3835 TGGCCA TGG^CCA
4162 TGGCCA
BbeI 112 GGCGCC GGCGC^C
833 GGCGCC
BlpI 2326 GCTCAGC GC^T[ACGT]AGC
4472 GCTAAGC
Bpu1102I 2326 GCTCAGC GC^T[ACGT]AGC
4472 GCTAAGC
BseAI 3772 TCCGGA T^CCGGA
4258 TCCGGA
BspEI 3772 TCCGGA T^CCGGA
4258 TCCGGA
BssSI 2265 CACGAG CACGAG(-5/-1)
3294 CACGAG
BstYI 471 AGATCC [GA]^GATC[CT]
3238 AGATCC
Bsu36I 1800 CCTGAGG CC^T[ACGT]AGG
3602 CCTGAGG
CelII 2326 GCTCAGC GC^T[ACGT]AGC
4472 GCTAAGC
DrdI 2166 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3087 GACGGGGGTGTC
Eco81I 1800 CCTGAGG CC^T[ACGT]AGG
3602 CCTGAGG
KasI 112 GGCGCC G^GCGCC
833 GGCGCC
MflI 471 AGATCC [GA]^GATC[CT]
3238 AGATCC
MluNI 3835 TGGCCA TGG^CCA
4162 TGGCCA
MroI 3772 TCCGGA T^CCGGA
4258 TCCGGA
MscI 3835 TGGCCA TGG^CCA
4162 TGGCCA
NarI 112 GGCGCC GG^CGCC
833 GGCGCC
SapI 3552 GAAGAGC GCTCTTC(1/4)
548 GCTCTTC
SfiI 1418 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4154 GGCCGAGCTGGCC
SfoI 112 GGCGCC GGC^GCC
833 GGCGCC
StuI 579 AGGCCT AGG^CCT
2881 AGGCCT
Tsp509I 1768 AATT ^AATT
3155 AATT
XhoII 471 AGATCC [GA]^GATC[CT]
3238 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI Aor51HI ApaI
AspEI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XmaCI XmaI