##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII BciVI BclI BfrI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI BstBI CelII ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI FspI
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RsrII SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AseI 2253 ATTAAT AT^TAAT
AsnI 2253 ATTAAT AT^TAAT
Asp700I 3144 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3813 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2545 CCTAGG C^CTAGG
BcgI 1850 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2035 AGATCT A^GATCT
BlnI 2545 CCTAGG C^CTAGG
BsiEI 877 CGGTCG CG[GA][CT]^CG
BspHI 2411 TCATGA T^CATGA
BssHII 2799 GCGCGC G^CGCGC
Bst1107I 3105 GTATAC GTA^TAC
BstZ17I 3105 GTATAC GTA^TAC
DrdI 568 GACTTCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1589 CTCTTC CTCTTC(1/4)
EcoT22I 1154 ATGCAT ATGCA^T
HgaI 809 GACGC GACGC(5/10)
Ksp632I 1589 CTCTTC CTCTTC(1/4)
KspI 2719 CCGCGG CCGC^GG
NheI 604 GCTAGC G^CTAGC
NsiI 1154 ATGCAT ATGCA^T
PflFI 3813 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2253 ATTAAT AT^TAAT
RcaI 2411 TCATGA T^CATGA
SacII 2719 CCGCGG CCGC^GG
SalI 879 GTCGAC G^TCGAC
SapI 1588 GCTCTTC GCTCTTC(1/4)
ScaI 1611 AGTACT AGT^ACT
SmlI 2629 CTCAAG C^T[CT][GA]AG
Tth111I 3813 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3144 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1487 AAATTC [GA]^AATT[CT]
3834 AAATTC
ApaI 538 GGGCCC GGGCC^C
1813 GGGCCC
ApoI 1487 AAATTC [GA]^AATT[CT]
3834 AAATTC
BamHI 151 GGATCC G^GATCC
182 GGATCC
BsaI 2537 GAGACC GGTCTC(1/5)
3926 GAGACC
BspLU11I 290 ACATGT A^CATGT
3128 ACATGT
BsrBI 902 GAGCGG CCGCTC(-3/-3)
1364 GAGCGG
BsrDI 4079 CATTGC GCAATG(2/0)
1794 GCAATG
BssSI 3479 CACGAG CACGAG(-5/-1)
551 CTCGTG
BstXI 3448 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3876 CCAGCACTTTGG
DraI 2125 TTTAAA TTT^AAA
2984 TTTAAA
DraIII 813 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2313 CACACAGTG
HincII 879 GTCGAC GT[CT]^[GA]AC
3093 GTCAAC
HindII 879 GTCGAC GT[CT]^[GA]AC
3093 GTCAAC
NaeI 924 GCCGGC GCC^GGC
1131 GCCGGC
NcoI 438 CCATGG C^CATGG
1119 CCATGG
NdeI 3254 CATATG CA^TATG
3557 CATATG
NgoMIV 924 GCCGGC G^CCGGC
1131 GCCGGC
PpuMI 327 GGGACCC [GA]G^G[AT]CC[CT]
747 AGGACCC
PvuII 587 CAGCTG CAG^CTG
936 CAGCTG
SacI 1493 GAGCTC GAGCT^C
1685 GAGCTC
SmaI 68 CCCGGG CCC^GGG
4050 CCCGGG
StuI 1905 AGGCCT AGG^CCT
2148 AGGCCT
XcmI 2062 CCACGGGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2093 CCAGGCTATGCTTGG
XmaCI 68 CCCGGG C^CCGGG
4050 CCCGGG
XmaI 68 CCCGGG C^CCGGG
4050 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII