##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BanI BbeI BbrPI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 17 enzymes) #
###################################
AocI 3767 CCTGAGG CC^T[ACGT]AGG
BbvCI 3215 CCTCAGC CCTCAGC(-5/-2)
BciVI 340 GTATCC GTATCC(6/5)
BglII 428 AGATCT A^GATCT
BsaI 3528 GAGACC GGTCTC(1/5)
BsgI 3548 CTGCAC GTGCAG(16/14)
BstAPI 220 GCAGATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3767 CCTGAGG CC^T[ACGT]AGG
DraII 3742 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 3767 CCTGAGG CC^T[ACGT]AGG
EcoO109I 3742 AGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 161 GACGC GACGC(5/10)
HincII 3422 GTCAAC GT[CT]^[GA]AC
HindII 3422 GTCAAC GT[CT]^[GA]AC
NheI 3494 GCTAGC G^CTAGC
PpuMI 3742 AGGTCCT [GA]G^G[AT]CC[CT]
SspI 3582 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 396 GTCTAC GT^[AC][GT]AC
3203 GTCTAC
AciI 1003 CCGC CCGC(-3/-1)
2206 CCGC
AcsI 118 GAATTT [GA]^AATT[CT]
2999 AAATTC
ApoI 118 GAATTT [GA]^AATT[CT]
2999 AAATTC
AseI 1601 ATTAAT AT^TAAT
2804 ATTAAT
AsnI 1601 ATTAAT AT^TAAT
2804 ATTAAT
AspHI 2926 GAGCAC G[AT]GC[AT]^C
3474 GTGCTC
BalI 535 TGGCCA TGG^CCA
1738 TGGCCA
BamHI 879 GGATCC G^GATCC
2082 GGATCC
BanII 1152 GGGCTC G[GA]GC[CT]^C
2355 GGGCTC
BcnI 1565 CCGGG CC^[GC]GG
2768 CCGGG
BseRI 2971 GAGGAG GAGGAG(10/8)
3738 GAGGAG
BsiHKAI 2926 GAGCAC G[AT]GC[AT]^C
3474 GTGCTC
BspHI 3429 TCATGA T^CATGA
3696 TCATGA
BspLU11I 1371 ACATGT A^CATGT
2574 ACATGT
BsrDI 1492 GCAATG GCAATG(2/0)
2695 GCAATG
DsaI 1142 CCATGG C^C[GA][CT]GG
2345 CCATGG
EaeI 535 TGGCCA [CT]^GGCC[GA]
1738 TGGCCA
MluNI 535 TGGCCA TGG^CCA
1738 TGGCCA
MscI 535 TGGCCA TGG^CCA
1738 TGGCCA
NciI 1565 CCGGG CC^[GC]GG
2768 CCGGG
NcoI 1142 CCATGG C^CATGG
2345 CCATGG
PshBI 1601 ATTAAT AT^TAAT
2804 ATTAAT
RcaI 3429 TCATGA T^CATGA
3696 TCATGA
TaqI 79 TCGA T^CGA
3003 TCGA
TthHB8I 79 TCGA T^CGA
3003 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AclI AfaI AflIII AluI AlwI
AlwNI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI Psp1406I PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII