##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
BbrPI       BciVI       BfrI        BglI        BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraIII      Eco47III    Eco81I      EcoRI       
EcoT22I     FseI        HindIII     KpnI        MfeI        
MluI        MroI        MunI        NaeI        NcoI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcyI         3499  GGCGCC               G[GA]^CG[CT]C
AhdI          279  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       283  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         279  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2402  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1347  TGCGCA               TGC^GCA
AvrII        2435  CCTAGG               C^CTAGG
BalI         3140  TGGCCA               TGG^CCA
BamHI        2679  GGATCC               G^GATCC
BbeI         3499  GGCGCC               GGCGC^C
BbvCI        3324  CCTCAGC              CCTCAGC(-5/-2)
BcgI         3313  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1410  AGATCT               A^GATCT
BlnI         2435  CCTAGG               C^CTAGG
BlpI         3753  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3753  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1484  TACGTG               [CT]AC^GT[GA]
BsaHI        3499  GGCGCC               G[GA]^CG[CT]C
BsgI         3262  GTGCAG               GTGCAG(16/14)
BsiEI         756  CGGCCG               CG[GA][CT]^CG
BsmBI        2258  GAGACG               CGTCTC(1/5)
BspHI        2977  TCATGA               T^CATGA
BsrDI        3899  GCAATG               GCAATG(2/0)
BstXI        3467  CCAAAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        3753  GCTCAGC              GC^T[ACGT]AGC
EagI          756  CGGCCG               C^GGCCG
Eam1105I      279  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         756  CGGCCG               C^GGCCG
Eco52I        756  CGGCCG               C^GGCCG
EcoNI        2493  CCTGCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1347  TGCGCA               TGC^GCA
HaeII        3499  GGCGCC               [GA]GCGC^[CT]
HgaI         2402  GACGC                GACGC(5/10)
Hin1I        3499  GGCGCC               G[GA]^CG[CT]C
KasI         3499  GGCGCC               G^GCGCC
KspI         3104  CCGCGG               CCGC^GG
MluNI        3140  TGGCCA               TGG^CCA
MscI         3140  TGGCCA               TGG^CCA
NarI         3499  GGCGCC               GG^CGCC
NdeI         1440  CATATG               CA^TATG
PflFI        2402  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2977  TCATGA               T^CATGA
SacII        3104  CCGCGG               CCGC^GG
SbfI          692  CCTGCAGG             CCTGCA^GG
SfoI         3499  GGCGCC               GGC^GCC
SmaI         2759  CCCGGG               CCC^GGG
Sse8387I      692  CCTGCAGG             CCTGCA^GG
SwaI         2184  ATTTAAAT             ATTT^AAAT
Tth111I      2402  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2759  CCCGGG               C^CCGGG
XmaI         2759  CCCGGG               C^CCGGG
XmnI          283  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         2399  GTAGAC               GT^[AC][GT]AC
             3672  GTAGAC              
AlwNI        1532  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1968  CAGCATCTG           
BbsI          547  GAAGAC               GAAGAC(2/6)
              287  GTCTTC              
BclI         1082  TGATCA               T^GATCA
             3809  TGATCA              
BpuAI         547  GAAGAC               GAAGAC(2/6)
              287  GTCTTC              
BsaI          688  GAGACC               GGTCTC(1/5)
             3425  GGTCTC              
BseRI        2752  CTCCTC               GAGGAG(10/8)
             1138  GAGGAG              
BssSI         367  CTCGTG               CACGAG(-5/-1)
             3442  CTCGTG              
BstEII       2264  GGTTACC              G^GT[ACGT]ACC
             2763  GGTCACC             
BstPI        2264  GGTTACC              G^GT[ACGT]ACC
             2763  GGTCACC             
DsaI         3104  CCGCGG               C^C[GA][CT]GG
             3495  CCACGG              
EaeI          756  CGGCCG               [CT]^GGCC[GA]
             3140  TGGCCA              
EcoO65I      2264  GGTTACC              G^GT[ACGT]ACC
             2763  GGTCACC             
EcoRV         442  GATATC               GAT^ATC
              799  GATATC              
FbaI         1082  TGATCA               T^GATCA
             3809  TGATCA              
HpaI         1579  GTTAAC               GTT^AAC
             2459  GTTAAC              
NheI         1275  GCTAGC               G^CTAGC
             2888  GCTAGC              
PpuMI         650  GGGTCCT              [GA]G^G[AT]CC[CT]
              899  AGGTCCT             
PvuII        1270  CAGCTG               CAG^CTG
             2454  CAGCTG              
ScaI         1394  AGTACT               AGT^ACT
             3592  AGTACT              
SspI         1354  AATATT               AAT^ATT
             2816  AATATT              
StuI         1876  AGGCCT               AGG^CCT
             2428  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaBI       BsaJI       
BsaWI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspLU11I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DrdI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XhoII