##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BstBI BstEII BstPI CelII ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 2829 GGTACC G^GTACC
AhdI 1949 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2829 GGTACC G^GTACC
AspEI 1949 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 505 GGATCC G^GATCC
BbvCI 2013 CCTCAGC CCTCAGC(-5/-2)
BclI 315 TGATCA T^GATCA
BglII 265 AGATCT A^GATCT
BsrFI 4035 GCCGGT [GA]^CCGG[CT]
BssHII 3526 GCGCGC G^CGCGC
Cfr10I 4035 GCCGGT [GA]^CCGG[CT]
DrdI 3651 GACAACGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1949 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3553 ATGCAT ATGCA^T
FbaI 315 TGATCA T^GATCA
HincII 3898 GTCAAC GT[CT]^[GA]AC
HindII 3898 GTCAAC GT[CT]^[GA]AC
KpnI 2829 GGTACC GGTAC^C
MspA1I 2403 CAGCTG C[AC]G^C[GT]G
NdeI 1545 CATATG CA^TATG
NsiI 3553 ATGCAT ATGCA^T
PaeR7I 3342 CTCGAG C^TCGAG
PvuII 2403 CAGCTG CAG^CTG
SphI 3551 GCATGC GCATG^C
XhoI 3342 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 896 GTATAC GT^[AC][GT]AC
2941 GTATAC
AcyI 2025 GACGCC G[GA]^CG[CT]C
2602 GACGCC
ApaI 2560 GGGCCC GGGCC^C
3640 GGGCCC
BalI 468 TGGCCA TGG^CCA
3999 TGGCCA
BbsI 938 GAAGAC GAAGAC(2/6)
361 GTCTTC
BglI 233 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2512 GCCCCTGGGGC
BpuAI 938 GAAGAC GAAGAC(2/6)
361 GTCTTC
BsaHI 2025 GACGCC G[GA]^CG[CT]C
2602 GACGCC
BsaWI 1784 ACCGGA [AT]^CCGG[AT]
3457 TCCGGT
BssSI 227 CACGAG CACGAG(-5/-1)
3169 CTCGTG
Bst1107I 896 GTATAC GTA^TAC
2941 GTATAC
BstZ17I 896 GTATAC GTA^TAC
2941 GTATAC
HaeII 130 AGCGCC [GA]GCGC^[CT]
3273 GGCGCT
Hin1I 2025 GACGCC G[GA]^CG[CT]C
2602 GACGCC
MluNI 468 TGGCCA TGG^CCA
3999 TGGCCA
MscI 468 TGGCCA TGG^CCA
3999 TGGCCA
NspI 2455 GCATGT [GA]CATG^[CT]
3551 GCATGC
SacI 354 GAGCTC GAGCT^C
1666 GAGCTC
SmlI 620 CTTGAG C^T[CT][GA]AG
3342 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI