##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        AspI        AviII       AvrII       
BbeI        BbrPI       BcgI        BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BstBI       BstEII      BstPI       CelII       ClaI        
CpoI        DraI        DraIII      EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FseI        
FspI        HpaI        KasI        KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

Acc65I       2829  GGTACC               G^GTACC
AhdI         1949  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      2829  GGTACC               G^GTACC
AspEI        1949  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         505  GGATCC               G^GATCC
BbvCI        2013  CCTCAGC              CCTCAGC(-5/-2)
BclI          315  TGATCA               T^GATCA
BglII         265  AGATCT               A^GATCT
BsrFI        4035  GCCGGT               [GA]^CCGG[CT]
BssHII       3526  GCGCGC               G^CGCGC
Cfr10I       4035  GCCGGT               [GA]^CCGG[CT]
DrdI         3651  GACAACGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1949  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      3553  ATGCAT               ATGCA^T
FbaI          315  TGATCA               T^GATCA
HincII       3898  GTCAAC               GT[CT]^[GA]AC
HindII       3898  GTCAAC               GT[CT]^[GA]AC
KpnI         2829  GGTACC               GGTAC^C
MspA1I       2403  CAGCTG               C[AC]G^C[GT]G
NdeI         1545  CATATG               CA^TATG
NsiI         3553  ATGCAT               ATGCA^T
PaeR7I       3342  CTCGAG               C^TCGAG
PvuII        2403  CAGCTG               CAG^CTG
SphI         3551  GCATGC               GCATG^C
XhoI         3342  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          896  GTATAC               GT^[AC][GT]AC
             2941  GTATAC              
AcyI         2025  GACGCC               G[GA]^CG[CT]C
             2602  GACGCC              
ApaI         2560  GGGCCC               GGGCC^C
             3640  GGGCCC              
BalI          468  TGGCCA               TGG^CCA
             3999  TGGCCA              
BbsI          938  GAAGAC               GAAGAC(2/6)
              361  GTCTTC              
BglI          233  GCCCGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2512  GCCCCTGGGGC         
BpuAI         938  GAAGAC               GAAGAC(2/6)
              361  GTCTTC              
BsaHI        2025  GACGCC               G[GA]^CG[CT]C
             2602  GACGCC              
BsaWI        1784  ACCGGA               [AT]^CCGG[AT]
             3457  TCCGGT              
BssSI         227  CACGAG               CACGAG(-5/-1)
             3169  CTCGTG              
Bst1107I      896  GTATAC               GTA^TAC
             2941  GTATAC              
BstZ17I       896  GTATAC               GTA^TAC
             2941  GTATAC              
HaeII         130  AGCGCC               [GA]GCGC^[CT]
             3273  GGCGCT              
Hin1I        2025  GACGCC               G[GA]^CG[CT]C
             2602  GACGCC              
MluNI         468  TGGCCA               TGG^CCA
             3999  TGGCCA              
MscI          468  TGGCCA               TGG^CCA
             3999  TGGCCA              
NspI         2455  GCATGT               [GA]CATG^[CT]
             3551  GCATGC              
SacI          354  GAGCTC               GAGCT^C
             1666  GAGCTC              
SmlI          620  CTTGAG               C^T[CT][GA]AG
             3342  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
RcaI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI        XmnI