##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

Acc65I      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AviII       
AvrII       BbeI        BcgI        BclI        BlnI        
BlpI        Bpu1102I    BsiWI       BspLU11I    BsrBI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       CpoI        DraIII      DrdI        
Eco47III    Eco81I      EcoNI       EcoRI       EcoRV       
FbaI        FseI        FspI        HpaI        KasI        
KpnI        KspI        MluI        NarI        NheI        
NotI        NruI        NspV        PaeR7I      PinAI       
PmeI        PshAI       PvuI        RsrII       SacII       
SbfI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       Sse8387I    XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1935  GACGTC               GACGT^C
AclI         4051  AACGTT               AA^CGTT
AflII         545  CTTAAG               C^TTAAG
Asp700I      2297  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          275  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1316  TGGCCA               TGG^CCA
BbrPI        1109  CACGTG               CAC^GTG
BbvCI          79  CCTCAGC              CCTCAGC(-5/-2)
BfrI          545  CTTAAG               C^TTAAG
BglI          728  GCCTTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1109  CACGTG               [CT]AC^GT[GA]
BsaBI        1238  GATCTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1937  CGTCTC               CGTCTC(1/5)
BspDI        3250  ATCGAT               AT^CGAT
BssSI        1571  CACGAG               CACGAG(-5/-1)
BstEII       3432  GGTGACC              G^GT[ACGT]ACC
BstPI        3432  GGTGACC              G^GT[ACGT]ACC
BstXI         750  CCATGATAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         3250  ATCGAT               AT^CGAT
EcoO65I      3432  GGTGACC              G^GT[ACGT]ACC
HaeII          43  GGCGCT               [GA]GCGC^[CT]
HindIII       474  AAGCTT               A^AGCTT
MamI         1238  GATCTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1316  TGGCCA               TGG^CCA
MscI         1316  TGGCCA               TGG^CCA
NcoI         2801  CCATGG               C^CATGG
NdeI         3847  CATATG               CA^TATG
PacI         3702  TTAATTAA             TTAAT^TAA
PflFI         275  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1109  CACGTG               CAC^GTG
PmlI         1109  CACGTG               CAC^GTG
Psp1406I     4051  AACGTT               AA^CGTT
PstI         1116  CTGCAG               CTGCA^G
SacI         3196  GAGCTC               GAGCT^C
SapI         2027  GCTCTTC              GCTCTTC(1/4)
SmaI         1363  CCCGGG               CCC^GGG
SpeI         2068  ACTAGT               A^CTAGT
SwaI         2498  ATTTAAAT             ATTT^AAAT
Tth111I       275  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1554  CCACCTGCATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1363  CCCGGG               C^CCGGG
XmaI         1363  CCCGGG               C^CCGGG
XmnI         2297  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI           17  GTCGAC               GT^[AC][GT]AC
             2167  GTCGAC              
AccIII       1581  TCCGGA               T^CCGGA
             1925  TCCGGA              
AcyI         1935  GACGTC               G[GA]^CG[CT]C
             3091  GGCGTC              
AflIII       2299  ACGTGT               A^C[GA][CT]GT
             3961  ACGTGT              
BamHI        1578  GGATCC               G^GATCC
             3970  GGATCC              
BanI           37  GGTGCC               G^G[CT][GA]CC
              996  GGTGCC              
BciVI         450  GTATCC               GTATCC(6/5)
             1789  GTATCC              
BglII         531  AGATCT               A^GATCT
             1798  AGATCT              
BsaHI        1935  GACGTC               G[GA]^CG[CT]C
             3091  GGCGTC              
BsaI         3458  GAGACC               GGTCTC(1/5)
             2158  GGTCTC              
BseAI        1581  TCCGGA               T^CCGGA
             1925  TCCGGA              
BsiEI         144  CGGCCG               CG[GA][CT]^CG
              157  CGGCCG              
BspEI        1581  TCCGGA               T^CCGGA
             1925  TCCGGA              
BsrDI        1881  CATTGC               GCAATG(2/0)
             1250  GCAATG              
BsrFI          40  GCCGGC               [GA]^CCGG[CT]
              177  GCCGGC              
Cfr10I         40  GCCGGC               [GA]^CCGG[CT]
              177  GCCGGC              
DsaI         1942  CCACGG               C^C[GA][CT]GG
             2801  CCATGG              
EagI          144  CGGCCG               C^GGCCG
              157  CGGCCG              
EclXI         144  CGGCCG               C^GGCCG
              157  CGGCCG              
Eco52I        144  CGGCCG               C^GGCCG
              157  CGGCCG              
Hin1I        1935  GACGTC               G[GA]^CG[CT]C
             3091  GGCGTC              
MroI         1581  TCCGGA               T^CCGGA
             1925  TCCGGA              
NaeI           40  GCCGGC               GCC^GGC
              177  GCCGGC              
NgoMIV         40  GCCGGC               G^CCGGC
              177  GCCGGC              
PflMI         778  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3437  CCAAGGTTTGG         
SalI           17  GTCGAC               G^TCGAC
             2167  GTCGAC              
ScaI          612  AGTACT               AGT^ACT
             1092  AGTACT              
SexAI        1263  ACCAGGT              A^CC[AT]GGT
             1389  ACCTGGT             
SfcI          262  CTACAG               C^T[GA][CT]AG
             1116  CTGCAG              
SphI         3371  GCATGC               GCATG^C
             3740  GCATGC              
SspI         1829  AATATT               AAT^ATT
             4082  AATATT              
StuI          549  AGGCCT               AGG^CCT
             2945  AGGCCT              
Van91I        778  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3437  CCAAGGTTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        AspEI       AspHI       AvaI        AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspHI       BspMI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EaeI        Eam1105I    EarI        
EcoO109I    EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MfeI        
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MunI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PpuMI       PshBI       PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XbaI        XhoII