##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
AspEI AviII AvrII BbrPI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KspI MamI MluI
MroI NdeI NheI NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SexAI SfuI SgrAI SnaBI SspBI
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 3301 GGTACC G^GTACC
AcsI 1339 GAATTT [GA]^AATT[CT]
AflIII 3933 ACATGT A^C[GA][CT]GT
Alw44I 2325 GTGCAC G^TGCAC
Aor51HI 366 AGCGCT AGC^GCT
ApaLI 2325 GTGCAC G^TGCAC
ApoI 1339 GAATTT [GA]^AATT[CT]
Asp718I 3301 GGTACC G^GTACC
BalI 3925 TGGCCA TGG^CCA
BamHI 3580 GGATCC G^GATCC
BbsI 2193 GAAGAC GAAGAC(2/6)
BbvCI 3320 GCTGAGG CCTCAGC(-5/-2)
BcgI 4312 GCACAGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2588 AGATCT A^GATCT
BpuAI 2193 GAAGAC GAAGAC(2/6)
BspLU11I 3933 ACATGT A^CATGT
BsrDI 3939 GCAATG GCAATG(2/0)
BstAPI 545 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 4275 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 366 AGCGCT AGC^GCT
HindIII 4421 AAGCTT A^AGCTT
KpnI 3301 GGTACC GGTAC^C
MfeI 1291 CAATTG C^AATTG
MluNI 3925 TGGCCA TGG^CCA
MscI 3925 TGGCCA TGG^CCA
MunI 1291 CAATTG C^AATTG
NruI 442 TCGCGA TCG^CGA
PflMI 4064 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1899 GCTCTTC GCTCTTC(1/4)
SbfI 3290 CCTGCAGG CCTGCA^GG
ScaI 4356 AGTACT AGT^ACT
SfiI 3362 GGCCAAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 373 CCCGGG CCC^GGG
SphI 1895 GCATGC GCATG^C
Sse8387I 3290 CCTGCAGG CCTGCA^GG
SspI 4408 AATATT AAT^ATT
TfiI 3531 GATTC G^A[AT]TC
Van91I 4064 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3913 CCAACCCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 373 CCCGGG C^CCGGG
XmaI 373 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 546 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3315 CAGAGGCTG
Asp700I 1318 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1914 GAAGGTTTTC
AspI 3975 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4346 GACACTGTC
AvaI 373 CCCGGG C^[CT]CG[GA]G
2045 CCCGAG
BlpI 2157 GCTCAGC GC^T[ACGT]AGC
2292 GCTGAGC
Bpu1102I 2157 GCTCAGC GC^T[ACGT]AGC
2292 GCTGAGC
BsoBI 373 CCCGGG C^[CT]CG[GA]G
2045 CCCGAG
BsrFI 340 GCCGGC [GA]^CCGG[CT]
2860 GCCGGC
CelII 2157 GCTCAGC GC^T[ACGT]AGC
2292 GCTGAGC
Cfr10I 340 GCCGGC [GA]^CCGG[CT]
2860 GCCGGC
EcoNI 891 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3584 CCTCAGGGAGG
HgaI 513 GACGC GACGC(5/10)
4323 GCGTC
HincII 2538 GTCAAC GT[CT]^[GA]AC
2689 GTCAAC
HindII 2538 GTCAAC GT[CT]^[GA]AC
2689 GTCAAC
MaeII 2405 ACGT A^CGT
2618 ACGT
NaeI 340 GCCGGC GCC^GGC
2860 GCCGGC
NcoI 3922 CCATGG C^CATGG
4330 CCATGG
NgoMIV 340 GCCGGC G^CCGGC
2860 GCCGGC
PflFI 3975 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4346 GACACTGTC
SpeI 278 ACTAGT A^CTAGT
4369 ACTAGT
Tth111I 3975 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4346 GACACTGTC
XmnI 1318 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1914 GAAGGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI AspHI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII