################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AflII AgeI Aor51HI AscI Asp718I AviII BbeI BbrPI BfrI BsaAI BsaBI BsiWI BsmBI BspDI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I FseI FspI HindIII KasI KpnI MamI MluI NaeI NarI NgoMIV NheI NotI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SalI ScaI SfiI SfoI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 3285 TCCGGA T^CCGGA AclI 2325 AACGTT AA^CGTT AhdI 1908 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1078 GTGCAC G^TGCAC ApaLI 1078 GTGCAC G^TGCAC AseI 947 ATTAAT AT^TAAT AsnI 947 ATTAAT AT^TAAT Asp700I 131 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1908 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 4058 CCTAGG C^CTAGG BciVI 2368 GTATCC GTATCC(6/5) BlnI 4058 CCTAGG C^CTAGG BsaI 1037 GAGACC GGTCTC(1/5) BseAI 3285 TCCGGA T^CCGGA BsiEI 3766 CGACCG CG[GA][CT]^CG BspEI 3285 TCCGGA T^CCGGA BspHI 444 TCATGA T^CATGA BspLU11I 576 ACATGT A^CATGT BstBI 1617 TTCGAA TT^CGAA BstXI 711 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 2195 TTTAAA TTT^AAA Eam1105I 1908 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2024 GAATTC G^AATTC EcoRV 1706 GATATC GAT^ATC HpaI 2038 GTTAAC GTT^AAC KspI 1368 CCGCGG CCGC^GG MfeI 2433 CAATTG C^AATTG MroI 3285 TCCGGA T^CCGGA MunI 2433 CAATTG C^AATTG NspV 1617 TTCGAA TT^CGAA PshBI 947 ATTAAT AT^TAAT Psp1406I 2325 AACGTT AA^CGTT RcaI 444 TCATGA T^CATGA SacII 1368 CCGCGG CCGC^GG SexAI 3324 ACCTGGT A^CC[AT]GGT SfuI 1617 TTCGAA TT^CGAA SmaI 348 CCCGGG CCC^GGG SphI 4075 GCATGC GCATG^C XcmI 2532 CCATTGTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 348 CCCGGG C^CCGGG XmaI 348 CCCGGG C^CCGGG XmnI 131 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 2258 GTCTAC GT^[AC][GT]AC 3254 GTCTAC AcyI 2354 GGCGTC G[GA]^CG[CT]C 3500 GGCGTC ApaI 7 GGGCCC GGGCC^C 3481 GGGCCC AspI 530 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 797 GACATTGTC AvaI 348 CCCGGG C^[CT]CG[GA]G 1765 CCCGAG BbvCI 3356 CCTCAGC CCTCAGC(-5/-2) 2228 GCTGAGG BcgI 1805 CGAAGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 468 GCAAGGCCATCG BclI 1446 TGATCA T^GATCA 2248 TGATCA BglI 1558 GCCCCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1803 GCCGAAGAGGC BlpI 3596 GCTGAGC GC^T[ACGT]AGC 3912 GCTAAGC Bpu1102I 3596 GCTGAGC GC^T[ACGT]AGC 3912 GCTAAGC BsaHI 2354 GGCGTC G[GA]^CG[CT]C 3500 GGCGTC BsaWI 3285 TCCGGA [AT]^CCGG[AT] 3768 ACCGGA BsmI 226 GAATGC GAATGC(1/-1) 3447 GCATTC BsoBI 348 CCCGGG C^[CT]CG[GA]G 1765 CCCGAG BsrFI 2565 ACCGGC [GA]^CCGG[CT] 3534 ACCGGC BstAPI 985 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2575 GCATCGGCTGC BstEII 433 GGTCACC G^GT[ACGT]ACC 3383 GGTGACC BstPI 433 GGTCACC G^GT[ACGT]ACC 3383 GGTGACC CelII 3596 GCTGAGC GC^T[ACGT]AGC 3912 GCTAAGC Cfr10I 2565 ACCGGC [GA]^CCGG[CT] 3534 ACCGGC DraIII 739 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 2639 CACAATGTG EcoO65I 433 GGTCACC G^GT[ACGT]ACC 3383 GGTGACC EcoT22I 160 ATGCAT ATGCA^T 228 ATGCAT FbaI 1446 TGATCA T^GATCA 2248 TGATCA Hin1I 2354 GGCGTC G[GA]^CG[CT]C 3500 GGCGTC HincII 803 GTCAAC GT[CT]^[GA]AC 2038 GTTAAC HindII 803 GTCAAC GT[CT]^[GA]AC 2038 GTTAAC NcoI 2043 CCATGG C^CATGG 2298 CCATGG NdeI 860 CATATG CA^TATG 2594 CATATG NruI 1237 TCGCGA TCG^CGA 3376 TCGCGA NsiI 160 ATGCAT ATGCA^T 228 ATGCAT PflFI 530 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 797 GACATTGTC SbfI 1456 CCTGCAGG CCTGCA^GG 3460 CCTGCAGG SmlI 869 CTCAAG C^T[CT][GA]AG 1672 CTTGAG Sse8387I 1456 CCTGCAGG CCTGCA^GG 3460 CCTGCAGG Tth111I 530 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 797 GACATTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII