##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        Aor51HI     
AscI        Asp718I     AviII       BbeI        BbrPI       
BfrI        BsaAI       BsaBI       BsiWI       BsmBI       
BspDI       BssHII      BssSI       Bst1107I    BstZ17I     
ClaI        CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      FseI        FspI        HindIII     
KasI        KpnI        MamI        MluI        NaeI        
NarI        NgoMIV      NheI        NotI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PvuI        RsrII       SalI        ScaI        
SfiI        SfoI        SgrAI       SnaBI       SpeI        
SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII       3285  TCCGGA               T^CCGGA
AclI         2325  AACGTT               AA^CGTT
AhdI         1908  GACCCATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1078  GTGCAC               G^TGCAC
ApaLI        1078  GTGCAC               G^TGCAC
AseI          947  ATTAAT               AT^TAAT
AsnI          947  ATTAAT               AT^TAAT
Asp700I       131  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1908  GACCCATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        4058  CCTAGG               C^CTAGG
BciVI        2368  GTATCC               GTATCC(6/5)
BlnI         4058  CCTAGG               C^CTAGG
BsaI         1037  GAGACC               GGTCTC(1/5)
BseAI        3285  TCCGGA               T^CCGGA
BsiEI        3766  CGACCG               CG[GA][CT]^CG
BspEI        3285  TCCGGA               T^CCGGA
BspHI         444  TCATGA               T^CATGA
BspLU11I      576  ACATGT               A^CATGT
BstBI        1617  TTCGAA               TT^CGAA
BstXI         711  CCATGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         2195  TTTAAA               TTT^AAA
Eam1105I     1908  GACCCATTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2024  GAATTC               G^AATTC
EcoRV        1706  GATATC               GAT^ATC
HpaI         2038  GTTAAC               GTT^AAC
KspI         1368  CCGCGG               CCGC^GG
MfeI         2433  CAATTG               C^AATTG
MroI         3285  TCCGGA               T^CCGGA
MunI         2433  CAATTG               C^AATTG
NspV         1617  TTCGAA               TT^CGAA
PshBI         947  ATTAAT               AT^TAAT
Psp1406I     2325  AACGTT               AA^CGTT
RcaI          444  TCATGA               T^CATGA
SacII        1368  CCGCGG               CCGC^GG
SexAI        3324  ACCTGGT              A^CC[AT]GGT
SfuI         1617  TTCGAA               TT^CGAA
SmaI          348  CCCGGG               CCC^GGG
SphI         4075  GCATGC               GCATG^C
XcmI         2532  CCATTGTGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         348  CCCGGG               C^CCGGG
XmaI          348  CCCGGG               C^CCGGG
XmnI          131  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccI         2258  GTCTAC               GT^[AC][GT]AC
             3254  GTCTAC              
AcyI         2354  GGCGTC               G[GA]^CG[CT]C
             3500  GGCGTC              
ApaI            7  GGGCCC               GGGCC^C
             3481  GGGCCC              
AspI          530  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              797  GACATTGTC           
AvaI          348  CCCGGG               C^[CT]CG[GA]G
             1765  CCCGAG              
BbvCI        3356  CCTCAGC              CCTCAGC(-5/-2)
             2228  GCTGAGG             
BcgI         1805  CGAAGAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              468  GCAAGGCCATCG        
BclI         1446  TGATCA               T^GATCA
             2248  TGATCA              
BglI         1558  GCCCCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1803  GCCGAAGAGGC         
BlpI         3596  GCTGAGC              GC^T[ACGT]AGC
             3912  GCTAAGC             
Bpu1102I     3596  GCTGAGC              GC^T[ACGT]AGC
             3912  GCTAAGC             
BsaHI        2354  GGCGTC               G[GA]^CG[CT]C
             3500  GGCGTC              
BsaWI        3285  TCCGGA               [AT]^CCGG[AT]
             3768  ACCGGA              
BsmI          226  GAATGC               GAATGC(1/-1)
             3447  GCATTC              
BsoBI         348  CCCGGG               C^[CT]CG[GA]G
             1765  CCCGAG              
BsrFI        2565  ACCGGC               [GA]^CCGG[CT]
             3534  ACCGGC              
BstAPI        985  GCAGGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2575  GCATCGGCTGC         
BstEII        433  GGTCACC              G^GT[ACGT]ACC
             3383  GGTGACC             
BstPI         433  GGTCACC              G^GT[ACGT]ACC
             3383  GGTGACC             
CelII        3596  GCTGAGC              GC^T[ACGT]AGC
             3912  GCTAAGC             
Cfr10I       2565  ACCGGC               [GA]^CCGG[CT]
             3534  ACCGGC              
DraIII        739  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2639  CACAATGTG           
EcoO65I       433  GGTCACC              G^GT[ACGT]ACC
             3383  GGTGACC             
EcoT22I       160  ATGCAT               ATGCA^T
              228  ATGCAT              
FbaI         1446  TGATCA               T^GATCA
             2248  TGATCA              
Hin1I        2354  GGCGTC               G[GA]^CG[CT]C
             3500  GGCGTC              
HincII        803  GTCAAC               GT[CT]^[GA]AC
             2038  GTTAAC              
HindII        803  GTCAAC               GT[CT]^[GA]AC
             2038  GTTAAC              
NcoI         2043  CCATGG               C^CATGG
             2298  CCATGG              
NdeI          860  CATATG               CA^TATG
             2594  CATATG              
NruI         1237  TCGCGA               TCG^CGA
             3376  TCGCGA              
NsiI          160  ATGCAT               ATGCA^T
              228  ATGCAT              
PflFI         530  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              797  GACATTGTC           
SbfI         1456  CCTGCAGG             CCTGCA^GG
             3460  CCTGCAGG            
SmlI          869  CTCAAG               C^T[CT][GA]AG
             1672  CTTGAG              
Sse8387I     1456  CCTGCAGG             CCTGCA^GG
             3460  CCTGCAGG            
Tth111I       530  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              797  GACATTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
AspHI       AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    Bsp1407I    BspMI       
BsrBI       BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SspBI       StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII