##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BclI BlnI
BseAI BsiWI BspEI BssHII Bst1107I
BstBI BstZ17I Bsu36I CpoI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoRI EcoT22I FbaI FspI HindIII
HpaI KspI MroI NheI NotI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3090 GGTACC G^GTACC
ApaI 1026 GGGCCC GGGCC^C
Asp700I 3507 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3090 GGTACC G^GTACC
BbsI 1863 GAAGAC GAAGAC(2/6)
BciVI 116 GTATCC GTATCC(6/5)
BglII 1070 AGATCT A^GATCT
BlpI 588 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 588 GCTGAGC GC^T[ACGT]AGC
BpuAI 1863 GAAGAC GAAGAC(2/6)
BsaAI 2316 CACGTA [CT]AC^GT[GA]
BsaBI 1746 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1494 CGACCG CG[GA][CT]^CG
BsmI 2373 GAATGC GAATGC(1/-1)
Bsp1407I 2877 TGTACA T^GTACA
BspDI 901 ATCGAT AT^CGAT
BspHI 3911 TCATGA T^CATGA
BsrGI 2877 TGTACA T^GTACA
CelII 588 GCTGAGC GC^T[ACGT]AGC
ClaI 901 ATCGAT AT^CGAT
DrdI 1099 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 898 GATATC GAT^ATC
FseI 33 GGCCGGCC GGCCGG^CC
HincII 130 GTCGAC GT[CT]^[GA]AC
HindII 130 GTCGAC GT[CT]^[GA]AC
KpnI 3090 GGTACC GGTAC^C
MamI 1746 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1271 CAATTG C^AATTG
MluI 2365 ACGCGT A^CGCGT
MunI 1271 CAATTG C^AATTG
NaeI 34 GCCGGC GCC^GGC
NcoI 3219 CCATGG C^CATGG
NdeI 1685 CATATG CA^TATG
NgoMIV 34 GCCGGC G^CCGGC
NruI 3692 TCGCGA TCG^CGA
PaeR7I 527 CTCGAG C^TCGAG
PflMI 888 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3911 TCATGA T^CATGA
SalI 130 GTCGAC G^TCGAC
SapI 1042 GAAGAGC GCTCTTC(1/4)
ScaI 1602 AGTACT AGT^ACT
SspBI 2877 TGTACA T^GTACA
Van91I 888 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 527 CTCGAG C^TCGAG
XmnI 3507 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 130 GTCGAC GT^[AC][GT]AC
2235 GTCTAC
AcyI 296 GGCGCC G[GA]^CG[CT]C
614 GGCGCC
Aor51HI 592 AGCGCT AGC^GCT
2633 AGCGCT
BbeI 296 GGCGCC GGCGC^C
614 GGCGCC
BglI 83 GCCAGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4140 GCCAACGAGGC
BsaHI 296 GGCGCC G[GA]^CG[CT]C
614 GGCGCC
BsaWI 3130 ACCGGA [AT]^CCGG[AT]
3522 ACCGGA
BsmBI 236 GAGACG CGTCTC(1/5)
2362 GAGACG
BspLU11I 3275 ACATGT A^CATGT
3476 ACATGT
BsrFI 34 GCCGGC [GA]^CCGG[CT]
4004 ACCGGC
BstAPI 170 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
622 GCACGACTTGC
BstEII 267 GGTAACC G^GT[ACGT]ACC
1289 GGTCACC
BstPI 267 GGTAACC G^GT[ACGT]ACC
1289 GGTCACC
BstXI 2039 CCATTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3080 CCAAGTTGTTGG
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
4004 ACCGGC
DraIII 1832 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1870 CACCCTGTG
Eco47III 592 AGCGCT AGC^GCT
2633 AGCGCT
EcoO65I 267 GGTAACC G^GT[ACGT]ACC
1289 GGTCACC
Hin1I 296 GGCGCC G[GA]^CG[CT]C
614 GGCGCC
KasI 296 GGCGCC G^GCGCC
614 GGCGCC
NarI 296 GGCGCC GG^CGCC
614 GGCGCC
PpuMI 720 AGGACCC [GA]G^G[AT]CC[CT]
1630 AGGTCCT
SfoI 296 GGCGCC GGC^GCC
614 GGCGCC
SmaI 5 CCCGGG CCC^GGG
3386 CCCGGG
SphI 2478 GCATGC GCATG^C
4025 GCATGC
XmaCI 5 CCCGGG C^CCGGG
3386 CCCGGG
XmaI 5 CCCGGG C^CCGGG
3386 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcgI BcnI BfaI
BfrI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII