##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AviII
BamHI BbeI BbrPI BcgI BcnI
BfrI BglII BsaBI BsaHI BsiEI
BsiWI BspDI BspLU11I BssHII BssSI
BstBI BstYI Bsu36I ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MamI MflI MluI NarI NciI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SwaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 1024 CGCG CG^CG
AseI 1845 ATTAAT AT^TAAT
AsnI 1845 ATTAAT AT^TAAT
AvrII 1712 CCTAGG C^CTAGG
BclI 4048 TGATCA T^GATCA
BglI 803 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1712 CCTAGG C^CTAGG
BlpI 3596 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3596 GCTTAGC GC^T[ACGT]AGC
BsmBI 1262 GAGACG CGTCTC(1/5)
Bsp1407I 2096 TGTACA T^GTACA
BspHI 1254 TCATGA T^CATGA
BsrBI 1010 GAGCGG CCGCTC(-3/-3)
BsrDI 622 GCAATG GCAATG(2/0)
BsrGI 2096 TGTACA T^GTACA
BstEII 4105 GGTTACC G^GT[ACGT]ACC
BstPI 4105 GGTTACC G^GT[ACGT]ACC
BstUI 1024 CGCG CG^CG
CelII 3596 GCTTAGC GC^T[ACGT]AGC
DrdI 300 GACTTTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 740 CCACGG C^C[GA][CT]GG
EcoO65I 4105 GGTTACC G^GT[ACGT]ACC
EcoT14I 1712 CCTAGG C^C[AT][AT]GG
EcoT22I 3770 ATGCAT ATGCA^T
FbaI 4048 TGATCA T^GATCA
HaeII 180 GGCGCT [GA]GCGC^[CT]
HindIII 2439 AAGCTT A^AGCTT
MfeI 3933 CAATTG C^AATTG
MspA1I 1245 CCGCTG C[AC]G^C[GT]G
MunI 3933 CAATTG C^AATTG
MvnI 1024 CGCG CG^CG
NaeI 1106 GCCGGC GCC^GGC
NgoMIV 1106 GCCGGC G^CCGGC
NsiI 3770 ATGCAT ATGCA^T
PshBI 1845 ATTAAT AT^TAAT
RcaI 1254 TCATGA T^CATGA
SapI 1216 GAAGAGC GCTCTTC(1/4)
SnaBI 3379 TACGTA TAC^GTA
SpeI 2815 ACTAGT A^CTAGT
SspBI 2096 TGTACA T^GTACA
StyI 1712 CCTAGG C^C[AT][AT]GG
XcmI 127 CCACTAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 344 TCCGGA T^CCGGA
1004 TCCGGA
AhdI 847 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3830 GACTTCCAGTC
AspEI 847 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3830 GACTTCCAGTC
AspHI 2853 GTGCTC G[AT]GC[AT]^C
3507 GAGCAC
AvaI 528 CTCGGG C^[CT]CG[GA]G
3047 CCCGAG
BalI 760 TGGCCA TGG^CCA
3129 TGGCCA
BbsI 942 GAAGAC GAAGAC(2/6)
2417 GAAGAC
BbvCI 637 CCTCAGC CCTCAGC(-5/-2)
3038 CCTCAGC
BciVI 251 GGATAC GTATCC(6/5)
3382 GTATCC
BpuAI 942 GAAGAC GAAGAC(2/6)
2417 GAAGAC
BsaAI 3379 TACGTA [CT]AC^GT[GA]
3479 CACGTA
BseAI 344 TCCGGA T^CCGGA
1004 TCCGGA
BsiHKAI 2853 GTGCTC G[AT]GC[AT]^C
3507 GAGCAC
BsmI 357 GAATGC GAATGC(1/-1)
3294 GCATTC
BsoBI 528 CTCGGG C^[CT]CG[GA]G
3047 CCCGAG
BspEI 344 TCCGGA T^CCGGA
1004 TCCGGA
BsrFI 255 ACCGGC [GA]^CCGG[CT]
1106 GCCGGC
Bst1107I 2673 GTATAC GTA^TAC
4175 GTATAC
BstAPI 831 GCACATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3003 GCAACCTCTGC
BstZ17I 2673 GTATAC GTA^TAC
4175 GTATAC
Cfr10I 255 ACCGGC [GA]^CCGG[CT]
1106 GCCGGC
DraIII 832 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
2269 CACCCAGTG
Eam1105I 847 GACAACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3830 GACTTCCAGTC
FauI 1134 CCCGC CCCGC(4/6)
807 GCGGG
HincII 3827 GTTGAC GT[CT]^[GA]AC
3928 GTTGAC
HindII 3827 GTTGAC GT[CT]^[GA]AC
3928 GTTGAC
MluNI 760 TGGCCA TGG^CCA
3129 TGGCCA
MroI 344 TCCGGA T^CCGGA
1004 TCCGGA
MscI 760 TGGCCA TGG^CCA
3129 TGGCCA
PflMI 54 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
606 CCATTTGTTGG
SexAI 1049 ACCAGGT A^CC[AT]GGT
3303 ACCAGGT
SphI 2506 GCATGC GCATG^C
3772 GCATGC
SspI 102 AATATT AAT^ATT
1871 AATATT
StuI 881 AGGCCT AGG^CCT
1190 AGGCCT
Van91I 54 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
606 CCATTTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspI
AvaII BanI BanII BbvI BfaI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XbaI