##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
Aor51HI ApaI ApaLI Asp700I Asp718I
AspEI AspI AviII BamHI BbrPI
BbvCI BcgI BlpI Bpu1102I BsaAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspMI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FseI
FspI HpaI KpnI MluI NheI
NotI NruI NsiI NspV PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SbfI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 476 GTCGAC GT^[AC][GT]AC
AccIII 2270 TCCGGA T^CCGGA
AclI 2705 AACGTT AA^CGTT
AflII 3903 CTTAAG C^TTAAG
AflIII 1521 ACATGT A^C[GA][CT]GT
AscI 357 GGCGCGCC GG^CGCGCC
AseI 2181 ATTAAT AT^TAAT
AsnI 2181 ATTAAT AT^TAAT
AvrII 1089 CCTAGG C^CTAGG
BalI 3007 TGGCCA TGG^CCA
BciVI 2814 GTATCC GTATCC(6/5)
BfrI 3903 CTTAAG C^TTAAG
BglI 86 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3833 AGATCT A^GATCT
BlnI 1089 CCTAGG C^CTAGG
BsaWI 2270 TCCGGA [AT]^CCGG[AT]
BseAI 2270 TCCGGA T^CCGGA
BsmBI 78 GAGACG CGTCTC(1/5)
BspEI 2270 TCCGGA T^CCGGA
BspLU11I 1521 ACATGT A^CATGT
BssHII 358 GCGCGC G^CGCGC
EcoRI 1994 GAATTC G^AATTC
HgaI 80 GACGC GACGC(5/10)
MfeI 2725 CAATTG C^AATTG
MluNI 3007 TGGCCA TGG^CCA
MroI 2270 TCCGGA T^CCGGA
MscI 3007 TGGCCA TGG^CCA
MunI 2725 CAATTG C^AATTG
NcoI 133 CCATGG C^CATGG
NdeI 2107 CATATG CA^TATG
PacI 2235 TTAATTAA TTAAT^TAA
PshBI 2181 ATTAAT AT^TAAT
Psp1406I 2705 AACGTT AA^CGTT
PvuII 105 CAGCTG CAG^CTG
SacI 2634 GAGCTC GAGCT^C
SalI 476 GTCGAC G^TCGAC
ScaI 2640 AGTACT AGT^ACT
SmaI 403 CCCGGG CCC^GGG
SspI 2178 AATATT AAT^ATT
StuI 1778 AGGCCT AGG^CCT
XcmI 133 CCATGGAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 403 CCCGGG C^CCGGG
XmaI 403 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1994 GAATTC [GA]^AATT[CT]
2614 AAATTC
AcyI 398 GGCGCC G[GA]^CG[CT]C
3454 GGCGCC
AocI 534 CCTGAGG CC^T[ACGT]AGG
2511 CCTGAGG
ApoI 1994 GAATTC [GA]^AATT[CT]
2614 AAATTC
BbeI 398 GGCGCC GGCGC^C
3454 GGCGCC
BclI 2321 TGATCA T^GATCA
2755 TGATCA
BsaBI 3103 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3621 GATCATTATC
BsaHI 398 GGCGCC G[GA]^CG[CT]C
3454 GGCGCC
BsgI 3434 CTGCAC GTGCAG(16/14)
3810 GTGCAG
BsrBI 421 CCGCTC CCGCTC(-3/-3)
441 GAGCGG
BstAPI 101 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1062 GCAGAATCTGC
BstYI 1586 AGATCC [GA]^GATC[CT]
3833 AGATCT
Bsu36I 534 CCTGAGG CC^T[ACGT]AGG
2511 CCTGAGG
DraI 3745 TTTAAA TTT^AAA
3932 TTTAAA
Eco81I 534 CCTGAGG CC^T[ACGT]AGG
2511 CCTGAGG
FbaI 2321 TGATCA T^GATCA
2755 TGATCA
HaeII 398 GGCGCC [GA]GCGC^[CT]
3454 GGCGCC
Hin1I 398 GGCGCC G[GA]^CG[CT]C
3454 GGCGCC
KasI 398 GGCGCC G^GCGCC
3454 GGCGCC
KspI 68 CCGCGG CCGC^GG
325 CCGCGG
MamI 3103 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3621 GATCATTATC
MflI 1586 AGATCC [GA]^GATC[CT]
3833 AGATCT
NarI 398 GGCGCC GG^CGCC
3454 GGCGCC
SacII 68 CCGCGG CCGC^GG
325 CCGCGG
SapI 705 GCTCTTC GCTCTTC(1/4)
2190 GCTCTTC
SfoI 398 GGCGCC GGC^GCC
3454 GGCGCC
SgrAI 353 CGCCGGCG C[GA]^CCGG[CT]G
394 CGCCGGCG
TaqI 477 TCGA T^CGA
1369 TCGA
TthHB8I 477 TCGA T^CGA
1369 TCGA
XhoII 1586 AGATCC [GA]^GATC[CT]
3833 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI