################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbsI BbvCI BcgI BclI BfrI BglII BpuAI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BstBI ClaI CpoI DrdI Eam1105I EarI EcoRI EcoRV FbaI FspI HpaI KpnI Ksp632I MamI MboII MfeI MluI MroI MunI NdeI NheI NotI NspV PacI PinAI PmeI PshAI PshBI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI Sse8387I SspBI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AclI 3310 AACGTT AA^CGTT AcsI 3888 AAATTT [GA]^AATT[CT] Aor51HI 562 AGCGCT AGC^GCT ApoI 3888 AAATTT [GA]^AATT[CT] BamHI 269 GGATCC G^GATCC BciVI 2360 GTATCC GTATCC(6/5) BsaWI 1651 ACCGGA [AT]^CCGG[AT] BsmI 652 GAATGC GAATGC(1/-1) BsrDI 1362 GCAATG GCAATG(2/0) BstEII 740 GGTCACC G^GT[ACGT]ACC BstPI 740 GGTCACC G^GT[ACGT]ACC EagI 2681 CGGCCG C^GGCCG EclXI 2681 CGGCCG C^GGCCG Eco47III 562 AGCGCT AGC^GCT Eco52I 2681 CGGCCG C^GGCCG EcoNI 3334 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 740 GGTCACC G^GT[ACGT]ACC EcoT22I 2653 ATGCAT ATGCA^T FseI 2642 GGCCGGCC GGCCGG^CC HindIII 3787 AAGCTT A^AGCTT KspI 34 CCGCGG CCGC^GG NruI 149 TCGCGA TCG^CGA NsiI 2653 ATGCAT ATGCA^T PaeR7I 525 CTCGAG C^TCGAG Psp1406I 3310 AACGTT AA^CGTT SacI 258 GAGCTC GAGCT^C SacII 34 CCGCGG CCGC^GG SfiI 522 GGCCTCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 525 CTCGAG C^T[CT][GA]AG SpeI 3850 ACTAGT A^CTAGT SspI 3970 AATATT AAT^ATT XhoI 525 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AspI 159 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2157 GACGGTGTC AvrII 2273 CCTAGG C^CTAGG 3571 CCTAGG BalI 441 TGGCCA TGG^CCA 538 TGGCCA BbeI 1001 GGCGCC GGCGC^C 3072 GGCGCC BbrPI 2522 CACGTG CAC^GTG 3255 CACGTG BlnI 2273 CCTAGG C^CTAGG 3571 CCTAGG BlpI 2716 GCTCAGC GC^T[ACGT]AGC 2956 GCTGAGC Bpu1102I 2716 GCTCAGC GC^T[ACGT]AGC 2956 GCTGAGC BsiEI 2681 CGGCCG CG[GA][CT]^CG 2688 CGGTCG BssSI 699 CACGAG CACGAG(-5/-1) 2165 CACGAG Bst1107I 3876 GTATAC GTA^TAC 4017 GTATAC BstYI 269 GGATCC [GA]^GATC[CT] 2048 AGATCC BstZ17I 3876 GTATAC GTA^TAC 4017 GTATAC CelII 2716 GCTCAGC GC^T[ACGT]AGC 2956 GCTGAGC DraI 3421 TTTAAA TTT^AAA 3913 TTTAAA DraIII 1619 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 2007 CACTGAGTG HincII 341 GTCAAC GT[CT]^[GA]AC 1629 GTTGAC HindII 341 GTCAAC GT[CT]^[GA]AC 1629 GTTGAC KasI 1001 GGCGCC G^GCGCC 3072 GGCGCC MflI 269 GGATCC [GA]^GATC[CT] 2048 AGATCC MluNI 441 TGGCCA TGG^CCA 538 TGGCCA MscI 441 TGGCCA TGG^CCA 538 TGGCCA NarI 1001 GGCGCC GG^CGCC 3072 GGCGCC PflFI 159 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2157 GACGGTGTC PmaCI 2522 CACGTG CAC^GTG 3255 CACGTG PmlI 2522 CACGTG CAC^GTG 3255 CACGTG SfoI 1001 GGCGCC GGC^GCC 3072 GGCGCC StuI 919 AGGCCT AGG^CCT 1799 AGGCCT Tth111I 159 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2157 GACGGTGTC XhoII 269 GGATCC [GA]^GATC[CT] 2048 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XmaCI XmaI