##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
AhdI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       BbsI        BbvCI       
BcgI        BclI        BfrI        BglII       BpuAI       
BsaBI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      BstBI       ClaI        CpoI        DrdI        
Eam1105I    EarI        EcoRI       EcoRV       FbaI        
FspI        HpaI        KpnI        Ksp632I     MamI        
MboII       MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SnaBI       
Sse8387I    SspBI       SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AclI         3310  AACGTT               AA^CGTT
AcsI         3888  AAATTT               [GA]^AATT[CT]
Aor51HI       562  AGCGCT               AGC^GCT
ApoI         3888  AAATTT               [GA]^AATT[CT]
BamHI         269  GGATCC               G^GATCC
BciVI        2360  GTATCC               GTATCC(6/5)
BsaWI        1651  ACCGGA               [AT]^CCGG[AT]
BsmI          652  GAATGC               GAATGC(1/-1)
BsrDI        1362  GCAATG               GCAATG(2/0)
BstEII        740  GGTCACC              G^GT[ACGT]ACC
BstPI         740  GGTCACC              G^GT[ACGT]ACC
EagI         2681  CGGCCG               C^GGCCG
EclXI        2681  CGGCCG               C^GGCCG
Eco47III      562  AGCGCT               AGC^GCT
Eco52I       2681  CGGCCG               C^GGCCG
EcoNI        3334  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       740  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2653  ATGCAT               ATGCA^T
FseI         2642  GGCCGGCC             GGCCGG^CC
HindIII      3787  AAGCTT               A^AGCTT
KspI           34  CCGCGG               CCGC^GG
NruI          149  TCGCGA               TCG^CGA
NsiI         2653  ATGCAT               ATGCA^T
PaeR7I        525  CTCGAG               C^TCGAG
Psp1406I     3310  AACGTT               AA^CGTT
SacI          258  GAGCTC               GAGCT^C
SacII          34  CCGCGG               CCGC^GG
SfiI          522  GGCCTCGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          525  CTCGAG               C^T[CT][GA]AG
SpeI         3850  ACTAGT               A^CTAGT
SspI         3970  AATATT               AAT^ATT
XhoI          525  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AspI          159  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2157  GACGGTGTC           
AvrII        2273  CCTAGG               C^CTAGG
             3571  CCTAGG              
BalI          441  TGGCCA               TGG^CCA
              538  TGGCCA              
BbeI         1001  GGCGCC               GGCGC^C
             3072  GGCGCC              
BbrPI        2522  CACGTG               CAC^GTG
             3255  CACGTG              
BlnI         2273  CCTAGG               C^CTAGG
             3571  CCTAGG              
BlpI         2716  GCTCAGC              GC^T[ACGT]AGC
             2956  GCTGAGC             
Bpu1102I     2716  GCTCAGC              GC^T[ACGT]AGC
             2956  GCTGAGC             
BsiEI        2681  CGGCCG               CG[GA][CT]^CG
             2688  CGGTCG              
BssSI         699  CACGAG               CACGAG(-5/-1)
             2165  CACGAG              
Bst1107I     3876  GTATAC               GTA^TAC
             4017  GTATAC              
BstYI         269  GGATCC               [GA]^GATC[CT]
             2048  AGATCC              
BstZ17I      3876  GTATAC               GTA^TAC
             4017  GTATAC              
CelII        2716  GCTCAGC              GC^T[ACGT]AGC
             2956  GCTGAGC             
DraI         3421  TTTAAA               TTT^AAA
             3913  TTTAAA              
DraIII       1619  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2007  CACTGAGTG           
HincII        341  GTCAAC               GT[CT]^[GA]AC
             1629  GTTGAC              
HindII        341  GTCAAC               GT[CT]^[GA]AC
             1629  GTTGAC              
KasI         1001  GGCGCC               G^GCGCC
             3072  GGCGCC              
MflI          269  GGATCC               [GA]^GATC[CT]
             2048  AGATCC              
MluNI         441  TGGCCA               TGG^CCA
              538  TGGCCA              
MscI          441  TGGCCA               TGG^CCA
              538  TGGCCA              
NarI         1001  GGCGCC               GG^CGCC
             3072  GGCGCC              
PflFI         159  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2157  GACGGTGTC           
PmaCI        2522  CACGTG               CAC^GTG
             3255  CACGTG              
PmlI         2522  CACGTG               CAC^GTG
             3255  CACGTG              
SfoI         1001  GGCGCC               GGC^GCC
             3072  GGCGCC              
StuI          919  AGGCCT               AGG^CCT
             1799  AGGCCT              
Tth111I       159  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2157  GACGGTGTC           
XhoII         269  GGATCC               [GA]^GATC[CT]
             2048  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI