##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaHI       BsaI        
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BstEII      BstPI       BstUI       BstXI       Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        KspI        MaeII       MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        Tth111I     
Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          285  GTATAC               GT^[AC][GT]AC
Alw44I        861  GTGCAC               G^TGCAC
AlwNI        1348  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         861  GTGCAC               G^TGCAC
BbvCI        1528  CCTCAGC              CCTCAGC(-5/-2)
BglI           15  GCCAAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1158  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1158  GCTAAGC              GC^T[ACGT]AGC
BsaBI         255  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         451  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI         980  TCCGGT               [AT]^CCGG[AT]
BsgI          433  GTGCAG               GTGCAG(16/14)
BsmAI         217  GAGAC                GTCTC(1/5)
BsmFI        1106  GGGAC                GGGAC(10/14)
BspHI        1588  TCATGA               T^CATGA
BsrDI        1747  GCAATG               GCAATG(2/0)
Bst1107I      285  GTATAC               GTA^TAC
BstAPI        426  GCAGCTAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         337  TTCGAA               TT^CGAA
BstZ17I       285  GTATAC               GTA^TAC
CelII        1158  GCTAAGC              GC^T[ACGT]AGC
Cfr13I        784  GGCCC                G^G[ACGT]CC
DraII         783  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoNI        1353  CCTGATGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      783  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         558  GATATC               GAT^ATC
EcoT14I       451  CCAAGG               C^C[AT][AT]GG
FauI         1742  GCGGG                CCCGC(4/6)
HindIII      1453  AAGCTT               A^AGCTT
HphI          747  GGTGA                GGTGA(8/7)
MamI          255  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV          337  TTCGAA               TT^CGAA
PleI         1585  GAGTC                GAGTC(4/5)
RcaI         1588  TCATGA               T^CATGA
SapI         2015  GAAGAGC              GCTCTTC(1/4)
Sau96I        784  GGCCC                G^G[ACGT]CC
SexAI        1630  ACCTGGT              A^CC[AT]GGT
SfuI          337  TTCGAA               TT^CGAA
SmlI         1197  CTTGAG               C^T[CT][GA]AG
SspI          312  AATATT               AAT^ATT
StyI          451  CCAAGG               C^C[AT][AT]GG
SwaI          364  ATTTAAAT             ATTT^AAAT
Tsp45I       1928  GTCAC                ^GT[GC]AC
XbaI         1334  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          153  GTAC                 GT^AC
              870  GTAC                
Asp700I      1035  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2120  GAAACATTTC          
AspHI         861  GTGCAC               G[AT]GC[AT]^C
             1066  GAGCAC              
BalI          173  TGGCCA               TGG^CCA
              764  TGGCCA              
BmyI          861  GTGCAC               G[AGT]GC[ACT]^C
             1066  GAGCAC              
BpmI          928  CTCCAG               CTGGAG(16/14)
             1366  CTCCAG              
BseRI         190  CTCCTC               GAGGAG(10/8)
             1810  GAGGAG              
BsiHKAI       861  GTGCAC               G[AT]GC[AT]^C
             1066  GAGCAC              
Bsp1286I      861  GTGCAC               G[AGT]GC[ACT]^C
             1066  GAGCAC              
BssKI         329  CCAGG                ^CC[ACGT]GG
             1631  CCTGG               
BssSI        1582  CACGAG               CACGAG(-5/-1)
                6  CTCGTG              
BstF5I       1274  GGATG                GGATG(2/0)
             1470  GGATG               
BstNI         329  CCAGG                CC^[AT]GG
             1631  CCTGG               
BstYI         260  AGATCC               [GA]^GATC[CT]
              332  GGATCT              
DraI          365  TTTAAA               TTT^AAA
              884  TTTAAA              
EcoRII        329  CCAGG                ^CC[AT]GG
             1631  CCTGG               
FokI         1274  GGATG                GGATG(9/13)
             1470  GGATG               
HapII         981  CCGG                 C^CGG
             1536  CCGG                
HpaII         981  CCGG                 C^CGG
             1536  CCGG                
MflI          260  AGATCC               [GA]^GATC[CT]
              332  GGATCT              
MluNI         173  TGGCCA               TGG^CCA
              764  TGGCCA              
MscI          173  TGGCCA               TGG^CCA
              764  TGGCCA              
MspA1I        931  CAGCTG               C[AC]G^C[GT]G
             1171  CAGCTG              
MspI          981  CCGG                 C^CGG
             1536  CCGG                
MvaI          329  CCAGG                CC^[AT]GG
             1631  CCTGG               
NlaIII        221  CATG                 CATG^
             1589  CATG                
NlaIV          12  GGAGCC               GG[ACGT]^[ACGT]CC
             1128  GGAGCC              
PstI          280  CTGCAG               CTGCA^G
             1886  CTGCAG              
PvuII         931  CAGCTG               CAG^CTG
             1171  CAGCTG              
RsaI          153  GTAC                 GT^AC
              870  GTAC                
ScrFI         329  CCAGG                CC^[ACGT]GG
             1631  CCTGG               
XcmI         1558  CCACTAAACCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1792  CCATTGTTTTTGTGG     
XhoII         260  AGATCC               [GA]^GATC[CT]
              332  GGATCT              
XmnI         1035  GAATCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2120  GAAACATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BbvI        BfaI        BsiYI       BslI        BsrI        
Cac8I       DdeI        DpnII       EaeI        EarI        
Fnu4HI      HaeIII      HincII      HindII      HinfI       
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MwoI        
NdeII       Sau3AI      SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I