################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglII BlnI BpuAI BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BstEII BstPI BstUI BstXI Bsu36I CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeII MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 285 GTATAC GT^[AC][GT]AC Alw44I 861 GTGCAC G^TGCAC AlwNI 1348 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 861 GTGCAC G^TGCAC BbvCI 1528 CCTCAGC CCTCAGC(-5/-2) BglI 15 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1158 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1158 GCTAAGC GC^T[ACGT]AGC BsaBI 255 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 451 CCAAGG C^C[ACGT][ACGT]GG BsaWI 980 TCCGGT [AT]^CCGG[AT] BsgI 433 GTGCAG GTGCAG(16/14) BsmAI 217 GAGAC GTCTC(1/5) BsmFI 1106 GGGAC GGGAC(10/14) BspHI 1588 TCATGA T^CATGA BsrDI 1747 GCAATG GCAATG(2/0) Bst1107I 285 GTATAC GTA^TAC BstAPI 426 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 337 TTCGAA TT^CGAA BstZ17I 285 GTATAC GTA^TAC CelII 1158 GCTAAGC GC^T[ACGT]AGC Cfr13I 784 GGCCC G^G[ACGT]CC DraII 783 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoNI 1353 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 783 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 558 GATATC GAT^ATC EcoT14I 451 CCAAGG C^C[AT][AT]GG FauI 1742 GCGGG CCCGC(4/6) HindIII 1453 AAGCTT A^AGCTT HphI 747 GGTGA GGTGA(8/7) MamI 255 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspV 337 TTCGAA TT^CGAA PleI 1585 GAGTC GAGTC(4/5) RcaI 1588 TCATGA T^CATGA SapI 2015 GAAGAGC GCTCTTC(1/4) Sau96I 784 GGCCC G^G[ACGT]CC SexAI 1630 ACCTGGT A^CC[AT]GGT SfuI 337 TTCGAA TT^CGAA SmlI 1197 CTTGAG C^T[CT][GA]AG SspI 312 AATATT AAT^ATT StyI 451 CCAAGG C^C[AT][AT]GG SwaI 364 ATTTAAAT ATTT^AAAT Tsp45I 1928 GTCAC ^GT[GC]AC XbaI 1334 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 153 GTAC GT^AC 870 GTAC Asp700I 1035 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2120 GAAACATTTC AspHI 861 GTGCAC G[AT]GC[AT]^C 1066 GAGCAC BalI 173 TGGCCA TGG^CCA 764 TGGCCA BmyI 861 GTGCAC G[AGT]GC[ACT]^C 1066 GAGCAC BpmI 928 CTCCAG CTGGAG(16/14) 1366 CTCCAG BseRI 190 CTCCTC GAGGAG(10/8) 1810 GAGGAG BsiHKAI 861 GTGCAC G[AT]GC[AT]^C 1066 GAGCAC Bsp1286I 861 GTGCAC G[AGT]GC[ACT]^C 1066 GAGCAC BssKI 329 CCAGG ^CC[ACGT]GG 1631 CCTGG BssSI 1582 CACGAG CACGAG(-5/-1) 6 CTCGTG BstF5I 1274 GGATG GGATG(2/0) 1470 GGATG BstNI 329 CCAGG CC^[AT]GG 1631 CCTGG BstYI 260 AGATCC [GA]^GATC[CT] 332 GGATCT DraI 365 TTTAAA TTT^AAA 884 TTTAAA EcoRII 329 CCAGG ^CC[AT]GG 1631 CCTGG FokI 1274 GGATG GGATG(9/13) 1470 GGATG HapII 981 CCGG C^CGG 1536 CCGG HpaII 981 CCGG C^CGG 1536 CCGG MflI 260 AGATCC [GA]^GATC[CT] 332 GGATCT MluNI 173 TGGCCA TGG^CCA 764 TGGCCA MscI 173 TGGCCA TGG^CCA 764 TGGCCA MspA1I 931 CAGCTG C[AC]G^C[GT]G 1171 CAGCTG MspI 981 CCGG C^CGG 1536 CCGG MvaI 329 CCAGG CC^[AT]GG 1631 CCTGG NlaIII 221 CATG CATG^ 1589 CATG NlaIV 12 GGAGCC GG[ACGT]^[ACGT]CC 1128 GGAGCC PstI 280 CTGCAG CTGCA^G 1886 CTGCAG PvuII 931 CAGCTG CAG^CTG 1171 CAGCTG RsaI 153 GTAC GT^AC 870 GTAC ScrFI 329 CCAGG CC^[ACGT]GG 1631 CCTGG XcmI 1558 CCACTAAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1792 CCATTGTTTTTGTGG XhoII 260 AGATCC [GA]^GATC[CT] 332 GGATCT XmnI 1035 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2120 GAAACATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbvI BfaI BsiYI BslI BsrI Cac8I DdeI DpnII EaeI EarI Fnu4HI HaeIII HincII HindII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII Sau3AI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I