##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AclI AcyI AhdI Alw44I
AocI ApaI ApaLI AscI AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BcgI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsiEI
BsiWI BsmBI BsoBI BspDI BsrBI
BsrDI BssHII BssSI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV FauI FseI FspI
HgaI Hin1I KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 4079 GGTACC G^GTACC
AccII 95 CGCG CG^CG
AccIII 1919 TCCGGA T^CCGGA
AflIII 3593 ACATGT A^C[GA][CT]GT
AgeI 3816 ACCGGT A^CCGGT
Aor51HI 4 AGCGCT AGC^GCT
Asp718I 4079 GGTACC G^GTACC
BamHI 2985 GGATCC G^GATCC
BclI 3212 TGATCA T^GATCA
BcnI 30 CCGGG CC^[GC]GG
BseAI 1919 TCCGGA T^CCGGA
BsgI 3955 GTGCAG GTGCAG(16/14)
BspEI 1919 TCCGGA T^CCGGA
BspHI 2972 TCATGA T^CATGA
BspLU11I 3593 ACATGT A^CATGT
BspMI 334 ACCTGC ACCTGC(4/8)
BsrFI 3816 ACCGGT [GA]^CCGG[CT]
BstUI 95 CGCG CG^CG
BstXI 73 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 249 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 3816 ACCGGT [GA]^CCGG[CT]
DraII 2779 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 4084 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 826 GACTTGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4 AGCGCT AGC^GCT
EcoNI 561 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2779 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2010 GAATTC G^AATTC
FbaI 3212 TGATCA T^GATCA
HpaI 1319 GTTAAC GTT^AAC
KpnI 4079 GGTACC GGTAC^C
MroI 1919 TCCGGA T^CCGGA
MvnI 95 CGCG CG^CG
NciI 30 CCGGG CC^[GC]GG
NdeI 2308 CATATG CA^TATG
PflMI 1961 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3816 ACCGGT A^CCGGT
PpuMI 2779 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2972 TCATGA T^CATGA
SalI 1183 GTCGAC G^TCGAC
SapI 3016 GCTCTTC GCTCTTC(1/4)
ScaI 1084 AGTACT AGT^ACT
Van91I 1961 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1379 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 1088 CTTAAG C^TTAAG
1791 CTTAAG
AseI 3180 ATTAAT AT^TAAT
4342 ATTAAT
AsnI 3180 ATTAAT AT^TAAT
4342 ATTAAT
AvaII 2780 GGTCC G^G[AT]CC
3078 GGACC
BalI 251 TGGCCA TGG^CCA
2476 TGGCCA
BanI 4079 GGTACC G^G[CT][GA]CC
4089 GGTGCC
BbvCI 19 CCTCAGC CCTCAGC(-5/-2)
62 CCTCAGC
BfrI 1088 CTTAAG C^TTAAG
1791 CTTAAG
BsaWI 1919 TCCGGA [AT]^CCGG[AT]
3816 ACCGGT
BsmI 1133 GAATGC GAATGC(1/-1)
3345 GAATGC
BstAPI 306 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2408 GCATATATTGC
Cfr13I 2780 GGTCC G^G[ACGT]CC
3078 GGACC
EaeI 251 TGGCCA [CT]^GGCC[GA]
2476 TGGCCA
EarI 1596 CTCTTC CTCTTC(1/4)
3017 CTCTTC
HincII 1183 GTCGAC GT[CT]^[GA]AC
1319 GTTAAC
HindII 1183 GTCGAC GT[CT]^[GA]AC
1319 GTTAAC
HindIII 816 AAGCTT A^AGCTT
3967 AAGCTT
Ksp632I 1596 CTCTTC CTCTTC(1/4)
3017 CTCTTC
MfeI 1204 CAATTG C^AATTG
1870 CAATTG
MluNI 251 TGGCCA TGG^CCA
2476 TGGCCA
MscI 251 TGGCCA TGG^CCA
2476 TGGCCA
MunI 1204 CAATTG C^AATTG
1870 CAATTG
PshBI 3180 ATTAAT AT^TAAT
4342 ATTAAT
PstI 2773 CTGCAG CTGCA^G
3737 CTGCAG
SacI 1014 GAGCTC GAGCT^C
2793 GAGCTC
Sau96I 2780 GGTCC G^G[ACGT]CC
3078 GGACC
XcmI 70 CCACCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3851 CCAATGCAGTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
BanII BbsI BbvI BciVI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI Bst1107I BstF5I BstNI
BstYI BstZ17I CfoI DdeI DpnII
DraI DsaI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PvuII RsaI Sau3AI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI