##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI AseI AsnI Asp700I AvrII
BamHI BbeI BcgI BciVI BclI
BlnI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstZ17I ClaI
CpoI DraI Eco47III EcoT22I FbaI
FseI HpaI KasI KspI MfeI
MluI MroI MunI NarI NdeI
NotI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SfoI SgrAI
SnaBI SpeI SspBI SwaI TfiI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 99 GGTACC G^GTACC
AccI 899 GTCTAC GT^[AC][GT]AC
AcyI 3168 GGCGTC G[GA]^CG[CT]C
AflII 3809 CTTAAG C^TTAAG
AocI 2453 CCTCAGG CC^T[ACGT]AGG
Asp718I 99 GGTACC G^GTACC
AspI 4154 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 73 TGCGCA TGC^GCA
BbrPI 1789 CACGTG CAC^GTG
BfrI 3809 CTTAAG C^TTAAG
BsaAI 1789 CACGTG [CT]AC^GT[GA]
BsaBI 424 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3168 GGCGTC G[GA]^CG[CT]C
BsaWI 2496 ACCGGA [AT]^CCGG[AT]
BsiEI 87 CGGCCG CG[GA][CT]^CG
BsmI 2975 GCATTC GAATGC(1/-1)
BsrBI 2270 GAGCGG CCGCTC(-3/-3)
BsrDI 1173 CATTGC GCAATG(2/0)
BssHII 278 GCGCGC G^CGCGC
BstBI 329 TTCGAA TT^CGAA
BstEII 733 GGTCACC G^GT[ACGT]ACC
BstPI 733 GGTCACC G^GT[ACGT]ACC
BstXI 4051 CCAGCTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2453 CCTCAGG CC^T[ACGT]AGG
DrdI 3303 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 87 CGGCCG C^GGCCG
EclXI 87 CGGCCG C^GGCCG
Eco52I 87 CGGCCG C^GGCCG
Eco81I 2453 CCTCAGG CC^T[ACGT]AGG
EcoO65I 733 GGTCACC G^GT[ACGT]ACC
EcoRI 385 GAATTC G^AATTC
EcoRV 307 GATATC GAT^ATC
FspI 73 TGCGCA TGC^GCA
Hin1I 3168 GGCGTC G[GA]^CG[CT]C
HincII 1199 GTCAAC GT[CT]^[GA]AC
HindII 1199 GTCAAC GT[CT]^[GA]AC
KpnI 99 GGTACC GGTAC^C
MamI 424 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 932 GCCGGC GCC^GGC
NgoMIV 932 GCCGGC G^CCGGC
NruI 4120 TCGCGA TCG^CGA
NspV 329 TTCGAA TT^CGAA
PaeR7I 3855 CTCGAG C^TCGAG
PflFI 4154 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1789 CACGTG CAC^GTG
PmlI 1789 CACGTG CAC^GTG
SbfI 175 CCTGCAGG CCTGCA^GG
SfiI 79 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 329 TTCGAA TT^CGAA
SphI 4037 GCATGC GCATG^C
Sse8387I 175 CCTGCAGG CCTGCA^GG
Tth111I 4154 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1971 TCTAGA T^CTAGA
XhoI 3855 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 885 ACATGT A^C[GA][CT]GT
1626 ACATGT
AhdI 503 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1795 GACAGACGGTC
Alw44I 167 GTGCAC G^TGCAC
635 GTGCAC
ApaLI 167 GTGCAC G^TGCAC
635 GTGCAC
AspEI 503 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1795 GACAGACGGTC
BalI 1851 TGGCCA TGG^CCA
3276 TGGCCA
BbsI 713 GTCTTC GAAGAC(2/6)
3476 GTCTTC
BpuAI 713 GTCTTC GAAGAC(2/6)
3476 GTCTTC
BseRI 587 CTCCTC GAGGAG(10/8)
2698 CTCCTC
BsmBI 1905 CGTCTC CGTCTC(1/5)
2676 CGTCTC
BspHI 3762 TCATGA T^CATGA
3940 TCATGA
BspLU11I 885 ACATGT A^CATGT
1626 ACATGT
BsrFI 932 GCCGGC [GA]^CCGG[CT]
1509 ACCGGC
BssSI 94 CTCGTG CACGAG(-5/-1)
1529 CTCGTG
Cfr10I 932 GCCGGC [GA]^CCGG[CT]
1509 ACCGGC
Eam1105I 503 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1795 GACAGACGGTC
HindIII 3907 AAGCTT A^AGCTT
3950 AAGCTT
MaeII 1790 ACGT A^CGT
2157 ACGT
MluNI 1851 TGGCCA TGG^CCA
3276 TGGCCA
MscI 1851 TGGCCA TGG^CCA
3276 TGGCCA
NcoI 1689 CCATGG C^CATGG
3490 CCATGG
NheI 2705 GCTAGC G^CTAGC
3501 GCTAGC
RcaI 3762 TCATGA T^CATGA
3940 TCATGA
ScaI 3832 AGTACT AGT^ACT
3962 AGTACT
SexAI 504 ACCTGGT A^CC[AT]GGT
903 ACCTGGT
SmaI 549 CCCGGG CCC^GGG
3589 CCCGGG
SspI 3839 AATATT AAT^ATT
3846 AATATT
XmaCI 549 CCCGGG C^CCGGG
3589 CCCGGG
XmaI 549 CCCGGG C^CCGGG
3589 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII