##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AlwNI AocI ApaI
AscI Asp718I AviII AvrII BalI
BamHI BbeI BlnI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstXI Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
Eco81I EcoO65I EcoT22I FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SfiI SfoI
SfuI SgrAI SnaBI SphI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1389 GTATAC GT^[AC][GT]AC
AflII 921 CTTAAG C^TTAAG
AhdI 1892 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3265 GTGCAC G^TGCAC
Aor51HI 23 AGCGCT AGC^GCT
ApaLI 3265 GTGCAC G^TGCAC
AspEI 1892 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1851 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1021 CACGTG CAC^GTG
BcgI 3240 GCATGTATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3159 GTATCC GTATCC(6/5)
BfrI 921 CTTAAG C^TTAAG
BglI 118 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1479 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2251 TCCGGT [AT]^CCGG[AT]
BseRI 1224 CTCCTC GAGGAG(10/8)
BsmAI 1983 GAGAC GTCTC(1/5)
BsmFI 1524 GTCCC GGGAC(10/14)
Bsp1407I 3165 TGTACA T^GTACA
BsrDI 1999 CATTGC GCAATG(2/0)
BsrGI 3165 TGTACA T^GTACA
Bst1107I 1389 GTATAC GTA^TAC
BstAPI 3189 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1389 GTATAC GTA^TAC
EaeI 1547 TGGCCG [CT]^GGCC[GA]
Eam1105I 1892 GACTCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 700 CTCTTC CTCTTC(1/4)
Eco47III 23 AGCGCT AGC^GCT
EcoNI 77 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 624 GATATC GAT^ATC
HaeII 23 AGCGCT [GA]GCGC^[CT]
HgaI 966 GCGTC GACGC(5/10)
Ksp632I 700 CTCTTC CTCTTC(1/4)
KspI 181 CCGCGG CCGC^GG
MamI 1479 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 3240 GCATGT [GA]CATG^[CT]
PflFI 1851 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1021 CACGTG CAC^GTG
PmlI 1021 CACGTG CAC^GTG
PvuII 720 CAGCTG CAG^CTG
SacII 181 CCGCGG CCGC^GG
SbfI 1535 CCTGCAGG CCTGCA^GG
SpeI 588 ACTAGT A^CTAGT
Sse8387I 1535 CCTGCAGG CCTGCA^GG
SspBI 3165 TGTACA T^GTACA
StuI 3113 AGGCCT AGG^CCT
SwaI 3446 ATTTAAAT ATTT^AAAT
Tth111I 1851 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 3265 GTGCAC G[AT]GC[AT]^C
3612 GAGCAC
BanII 177 GAGCCC G[GA]GC[CT]^C
186 GAGCCC
BbvCI 717 CCTCAGC CCTCAGC(-5/-2)
1046 GCTGAGG
BclI 304 TGATCA T^GATCA
2765 TGATCA
BglII 1613 AGATCT A^GATCT
1780 AGATCT
BsaAI 1021 CACGTG [CT]AC^GT[GA]
3137 TACGTG
BsiHKAI 3265 GTGCAC G[AT]GC[AT]^C
3612 GAGCAC
EcoRI 1402 GAATTC G^AATTC
1993 GAATTC
EcoT14I 1101 CCATGG C^C[AT][AT]GG
1544 CCATGG
FbaI 304 TGATCA T^GATCA
2765 TGATCA
HindIII 243 AAGCTT A^AGCTT
731 AAGCTT
NcoI 1101 CCATGG C^CATGG
1544 CCATGG
NheI 2090 GCTAGC G^CTAGC
2678 GCTAGC
ScaI 650 AGTACT AGT^ACT
3839 AGTACT
SexAI 1384 ACCAGGT A^CC[AT]GGT
2465 ACCTGGT
SmaI 128 CCCGGG CCC^GGG
197 CCCGGG
SmlI 921 CTTAAG C^T[CT][GA]AG
2797 CTCAAG
SspI 3365 AATATT AAT^ATT
3737 AATATT
StyI 1101 CCATGG C^C[AT][AT]GG
1544 CCATGG
TaqI 1076 TCGA T^CGA
1849 TCGA
TthHB8I 1076 TCGA T^CGA
1849 TCGA
XbaI 726 TCTAGA T^CTAGA
2983 TCTAGA
XcmI 1101 CCATGGAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1976 CCAACTGGAGACTGG
XmaCI 128 CCCGGG C^CCGGG
197 CCCGGG
XmaI 128 CCCGGG C^CCGGG
197 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AvaI AvaII BanI BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsgI BsiYI
BslI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI