################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AclI AflII Alw44I Aor51HI ApaI ApaLI AseI AsnI AviII AvrII BcgI BfrI BglI BlnI BsiWI BspDI BstBI ClaI CpoI DraIII Eco47III EcoRV FseI FspI MluI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SexAI SfiI SfuI SgrAI SmaI SnaBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 75 TCCGGA T^CCGGA AgeI 3618 ACCGGT A^CCGGT AhdI 8764 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AscI 187 GGCGCGCC GG^CGCGCC AspEI 8764 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 7021 GGCGCC GGCGC^C BbrPI 2300 CACGTG CAC^GTG BseAI 75 TCCGGA T^CCGGA BspEI 75 TCCGGA T^CCGGA BspLU11I 8108 ACATGT A^CATGT BssHII 188 GCGCGC G^CGCGC BstEII 1290 GGTTACC G^GT[ACGT]ACC BstPI 1290 GGTTACC G^GT[ACGT]ACC DraI 554 TTTAAA TTT^AAA DrdI 97 GACCAGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 8764 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 883 CCTCTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1290 GGTTACC G^GT[ACGT]ACC EcoRI 6083 GAATTC G^AATTC EcoT22I 2311 ATGCAT ATGCA^T HpaI 1709 GTTAAC GTT^AAC KasI 7021 GGCGCC G^GCGCC KspI 283 CCGCGG CCGC^GG MroI 75 TCCGGA T^CCGGA NarI 7021 GGCGCC GG^CGCC NdeI 6298 CATATG CA^TATG NsiI 2311 ATGCAT ATGCA^T PinAI 3618 ACCGGT A^CCGGT PmaCI 2300 CACGTG CAC^GTG PmlI 2300 CACGTG CAC^GTG PshAI 2353 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 904 GAGCTC GAGCT^C SacII 283 CCGCGG CCGC^GG SalI 3118 GTCGAC G^TCGAC SbfI 2567 CCTGCAGG CCTGCA^GG ScaI 4956 AGTACT AGT^ACT SfoI 7021 GGCGCC GGC^GCC SpeI 4667 ACTAGT A^CTAGT SphI 3224 GCATGC GCATG^C Sse8387I 2567 CCTGCAGG CCTGCA^GG XbaI 7388 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 458 GACGTC GACGT^C 755 GACGTC AflIII 2301 ACGTGT A^C[GA][CT]GT 8108 ACATGT AocI 835 CCTTAGG CC^T[ACGT]AGG 9016 CCTAAGG Asp700I 8030 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 8298 GAACTCATTC AspI 79 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 8062 GACTATGTC BalI 2920 TGGCCA TGG^CCA 6503 TGGCCA BbvCI 5241 GCTGAGG CCTCAGC(-5/-2) 9133 GCTGAGG BsaAI 1405 TACGTG [CT]AC^GT[GA] 2300 CACGTG BsaWI 75 TCCGGA [AT]^CCGG[AT] 3618 ACCGGT BsiEI 147 CGGCCG CG[GA][CT]^CG 203 CGGCCG BsmBI 8206 CGTCTC CGTCTC(1/5) 3431 GAGACG BstAPI 5388 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5748 GCAGCAGCTGC BstXI 5561 CCATGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6595 CCATCCCCTTGG Bsu36I 835 CCTTAGG CC^T[ACGT]AGG 9016 CCTAAGG EagI 147 CGGCCG C^GGCCG 203 CGGCCG EclXI 147 CGGCCG C^GGCCG 203 CGGCCG Eco52I 147 CGGCCG C^GGCCG 203 CGGCCG Eco81I 835 CCTTAGG CC^T[ACGT]AGG 9016 CCTAAGG MluNI 2920 TGGCCA TGG^CCA 6503 TGGCCA MscI 2920 TGGCCA TGG^CCA 6503 TGGCCA NaeI 181 GCCGGC GCC^GGC 919 GCCGGC NgoMIV 181 GCCGGC G^CCGGC 919 GCCGGC PflFI 79 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 8062 GACTATGTC PpuMI 2276 GGGTCCT [GA]G^G[AT]CC[CT] 3656 AGGTCCT SapI 2397 GAAGAGC GCTCTTC(1/4) 4551 GAAGAGC Tth111I 79 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 8062 GACTATGTC XmnI 8030 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 8298 GAACTCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI Bst1107I BstF5I BstNI BstUI BstYI BstZ17I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PaeR7I PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII