##################################
# NO CUT ENZYMES (   43 enzymes) #
##################################

AclI        AflII       Alw44I      Aor51HI     ApaI        
ApaLI       AseI        AsnI        AviII       AvrII       
BcgI        BfrI        BglI        BlnI        BsiWI       
BspDI       BstBI       ClaI        CpoI        DraIII      
Eco47III    EcoRV       FseI        FspI        MluI        
NotI        NruI        NspV        PacI        PmeI        
PshBI       Psp1406I    PvuI        RsrII       SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII         75  TCCGGA               T^CCGGA
AgeI         3618  ACCGGT               A^CCGGT
AhdI         8764  GACTTTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI          187  GGCGCGCC             GG^CGCGCC
AspEI        8764  GACTTTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         7021  GGCGCC               GGCGC^C
BbrPI        2300  CACGTG               CAC^GTG
BseAI          75  TCCGGA               T^CCGGA
BspEI          75  TCCGGA               T^CCGGA
BspLU11I     8108  ACATGT               A^CATGT
BssHII        188  GCGCGC               G^CGCGC
BstEII       1290  GGTTACC              G^GT[ACGT]ACC
BstPI        1290  GGTTACC              G^GT[ACGT]ACC
DraI          554  TTTAAA               TTT^AAA
DrdI           97  GACCAGACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     8764  GACTTTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         883  CCTCTGGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1290  GGTTACC              G^GT[ACGT]ACC
EcoRI        6083  GAATTC               G^AATTC
EcoT22I      2311  ATGCAT               ATGCA^T
HpaI         1709  GTTAAC               GTT^AAC
KasI         7021  GGCGCC               G^GCGCC
KspI          283  CCGCGG               CCGC^GG
MroI           75  TCCGGA               T^CCGGA
NarI         7021  GGCGCC               GG^CGCC
NdeI         6298  CATATG               CA^TATG
NsiI         2311  ATGCAT               ATGCA^T
PinAI        3618  ACCGGT               A^CCGGT
PmaCI        2300  CACGTG               CAC^GTG
PmlI         2300  CACGTG               CAC^GTG
PshAI        2353  GACGACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          904  GAGCTC               GAGCT^C
SacII         283  CCGCGG               CCGC^GG
SalI         3118  GTCGAC               G^TCGAC
SbfI         2567  CCTGCAGG             CCTGCA^GG
ScaI         4956  AGTACT               AGT^ACT
SfoI         7021  GGCGCC               GGC^GCC
SpeI         4667  ACTAGT               A^CTAGT
SphI         3224  GCATGC               GCATG^C
Sse8387I     2567  CCTGCAGG             CCTGCA^GG
XbaI         7388  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AatII         458  GACGTC               GACGT^C
              755  GACGTC              
AflIII       2301  ACGTGT               A^C[GA][CT]GT
             8108  ACATGT              
AocI          835  CCTTAGG              CC^T[ACGT]AGG
             9016  CCTAAGG             
Asp700I      8030  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             8298  GAACTCATTC          
AspI           79  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             8062  GACTATGTC           
BalI         2920  TGGCCA               TGG^CCA
             6503  TGGCCA              
BbvCI        5241  GCTGAGG              CCTCAGC(-5/-2)
             9133  GCTGAGG             
BsaAI        1405  TACGTG               [CT]AC^GT[GA]
             2300  CACGTG              
BsaWI          75  TCCGGA               [AT]^CCGG[AT]
             3618  ACCGGT              
BsiEI         147  CGGCCG               CG[GA][CT]^CG
              203  CGGCCG              
BsmBI        8206  CGTCTC               CGTCTC(1/5)
             3431  GAGACG              
BstAPI       5388  GCAGCAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5748  GCAGCAGCTGC         
BstXI        5561  CCATGCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6595  CCATCCCCTTGG        
Bsu36I        835  CCTTAGG              CC^T[ACGT]AGG
             9016  CCTAAGG             
EagI          147  CGGCCG               C^GGCCG
              203  CGGCCG              
EclXI         147  CGGCCG               C^GGCCG
              203  CGGCCG              
Eco52I        147  CGGCCG               C^GGCCG
              203  CGGCCG              
Eco81I        835  CCTTAGG              CC^T[ACGT]AGG
             9016  CCTAAGG             
MluNI        2920  TGGCCA               TGG^CCA
             6503  TGGCCA              
MscI         2920  TGGCCA               TGG^CCA
             6503  TGGCCA              
NaeI          181  GCCGGC               GCC^GGC
              919  GCCGGC              
NgoMIV        181  GCCGGC               G^CCGGC
              919  GCCGGC              
PflFI          79  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             8062  GACTATGTC           
PpuMI        2276  GGGTCCT              [GA]G^G[AT]CC[CT]
             3656  AGGTCCT             
SapI         2397  GAAGAGC              GCTCTTC(1/4)
             4551  GAAGAGC             
Tth111I        79  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             8062  GACTATGTC           
XmnI         8030  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             8298  GAACTCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  147 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcsI        
AcyI        AfaI        AluI        AlwI        AlwNI       
ApoI        Asp718I     AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbsI        BbvI        
BciVI       BclI        BcnI        BfaI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssKI       BssSI       Bst1107I    
BstF5I      BstNI       BstUI       BstYI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        KpnI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MfeI        MflI        MnlI        MseI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NheI        
NlaIII      NlaIV       NspI        PaeR7I      PflMI       
PleI        PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoI        XhoII