##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AviII AvrII
BalI BamHI BanI BbeI BglI
BlnI BlpI Bpu1102I BsaI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII Bst1107I BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI FseI FspI KasI
KpnI KspI MluI MluNI MroI
MscI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI Sse8387I StuI SwaI
Van91I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 500 GGCGTC G[GA]^CG[CT]C
AflII 1918 CTTAAG C^TTAAG
Alw44I 395 GTGCAC G^TGCAC
ApaLI 395 GTGCAC G^TGCAC
Asp700I 3421 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2418 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3628 CACGTG CAC^GTG
BbvCI 73 GCTGAGG CCTCAGC(-5/-2)
BciVI 1207 GGATAC GTATCC(6/5)
BclI 515 TGATCA T^GATCA
BfrI 1918 CTTAAG C^TTAAG
BsaAI 3628 CACGTG [CT]AC^GT[GA]
BsaHI 500 GGCGTC G[GA]^CG[CT]C
BsaWI 1925 ACCGGA [AT]^CCGG[AT]
BsgI 1316 CTGCAC GTGCAG(16/14)
BsmBI 502 CGTCTC CGTCTC(1/5)
BsmFI 1283 GGGAC GGGAC(10/14)
BspHI 2834 TCATGA T^CATGA
BspLU11I 3967 ACATGT A^CATGT
BsrFI 2525 ACCGGC [GA]^CCGG[CT]
BstBI 2366 TTCGAA TT^CGAA
BstEII 2610 GGTCACC G^GT[ACGT]ACC
BstPI 2610 GGTCACC G^GT[ACGT]ACC
CfoI 168 GCGC GCG^C
Cfr10I 2525 ACCGGC [GA]^CCGG[CT]
DraIII 3442 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2090 TGGCCG [CT]^GGCC[GA]
EcoNI 2731 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2610 GGTCACC G^GT[ACGT]ACC
EcoRV 756 GATATC GAT^ATC
EcoT22I 412 ATGCAT ATGCA^T
FbaI 515 TGATCA T^GATCA
HaeII 167 GGCGCT [GA]GCGC^[CT]
HhaI 168 GCGC GCG^C
Hin1I 500 GGCGTC G[GA]^CG[CT]C
HinP1I 168 GCGC G^CGC
MfeI 2283 CAATTG C^AATTG
MunI 2283 CAATTG C^AATTG
NsiI 412 ATGCAT ATGCA^T
NspV 2366 TTCGAA TT^CGAA
PflFI 2418 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3628 CACGTG CAC^GTG
PmlI 3628 CACGTG CAC^GTG
PpuMI 1788 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 2834 TCATGA T^CATGA
SfuI 2366 TTCGAA TT^CGAA
SmaI 1280 CCCGGG CCC^GGG
Tth111I 2418 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1280 CCCGGG C^CCGGG
XmaI 1280 CCCGGG C^CCGGG
XmnI 3421 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AseI 1893 ATTAAT AT^TAAT
3185 ATTAAT
AsnI 1893 ATTAAT AT^TAAT
3185 ATTAAT
AvaI 1280 CCCGGG C^[CT]CG[GA]G
2474 CCCGAG
BbsI 666 GAAGAC GAAGAC(2/6)
2521 GAAGAC
BcgI 3679 CGACTGTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
792 GCAAGTTCATCG
BglII 1593 AGATCT A^GATCT
2901 AGATCT
BpuAI 666 GAAGAC GAAGAC(2/6)
2521 GAAGAC
BseRI 1546 GAGGAG GAGGAG(10/8)
2782 GAGGAG
BsoBI 1280 CCCGGG C^[CT]CG[GA]G
2474 CCCGAG
Bsp1407I 3201 TGTACA T^GTACA
3735 TGTACA
BsrGI 3201 TGTACA T^GTACA
3735 TGTACA
BssSI 1454 CACGAG CACGAG(-5/-1)
1576 CACGAG
BstAPI 723 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1777 GCAGCTGCTGC
DsaI 809 CCATGG C^C[GA][CT]GG
1806 CCATGG
HgaI 501 GCGTC GACGC(5/10)
912 GCGTC
HindIII 643 AAGCTT A^AGCTT
2537 AAGCTT
HpaI 3245 GTTAAC GTT^AAC
3592 GTTAAC
NcoI 809 CCATGG C^CATGG
1806 CCATGG
PshBI 1893 ATTAAT AT^TAAT
3185 ATTAAT
SapI 2369 GAAGAGC GCTCTTC(1/4)
2791 GAAGAGC
SmlI 1918 CTTAAG C^T[CT][GA]AG
4106 CTTGAG
SpeI 231 ACTAGT A^CTAGT
1324 ACTAGT
SphI 1471 GCATGC GCATG^C
3277 GCATGC
SspBI 3201 TGTACA T^GTACA
3735 TGTACA
SspI 3695 AATATT AAT^ATT
4007 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaBI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII