##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI BcgI BglI BsaBI
BsiEI BsiWI BsmBI BspDI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstZ17I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I FseI
KpnI KspI MamI MfeI MluI
MunI NheI NotI NruI PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SgrAI SnaBI SphI
Sse8387I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 4220 GTAGAC GT^[AC][GT]AC
AccII 1644 CGCG CG^CG
AccIII 1135 TCCGGA T^CCGGA
AflII 1796 CTTAAG C^TTAAG
AocI 1042 CCTCAGG CC^T[ACGT]AGG
Asp700I 2198 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2002 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4265 TGCGCA TGC^GCA
AvrII 4225 CCTAGG C^CTAGG
BamHI 808 GGATCC G^GATCC
BbeI 716 GGCGCC GGCGC^C
BbrPI 2871 CACGTG CAC^GTG
BciVI 4360 GGATAC GTATCC(6/5)
BfrI 1796 CTTAAG C^TTAAG
BlnI 4225 CCTAGG C^CTAGG
BsaAI 2871 CACGTG [CT]AC^GT[GA]
BsaWI 1135 TCCGGA [AT]^CCGG[AT]
BseAI 1135 TCCGGA T^CCGGA
BsmI 461 GCATTC GAATGC(1/-1)
BspEI 1135 TCCGGA T^CCGGA
BsrDI 1557 CATTGC GCAATG(2/0)
BstBI 1457 TTCGAA TT^CGAA
BstEII 3413 GGTCACC G^GT[ACGT]ACC
BstPI 3413 GGTCACC G^GT[ACGT]ACC
BstUI 1644 CGCG CG^CG
BstXI 2451 CCATGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1042 CCTCAGG CC^T[ACGT]AGG
DrdI 2539 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1042 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3413 GGTCACC G^GT[ACGT]ACC
EcoRV 394 GATATC GAT^ATC
FspI 4265 TGCGCA TGC^GCA
HpaI 24 GTTAAC GTT^AAC
KasI 716 GGCGCC G^GCGCC
MroI 1135 TCCGGA T^CCGGA
MvnI 1644 CGCG CG^CG
NarI 716 GGCGCC GG^CGCC
NdeI 740 CATATG CA^TATG
NspV 1457 TTCGAA TT^CGAA
PaeR7I 3220 CTCGAG C^TCGAG
PflFI 2002 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2871 CACGTG CAC^GTG
PmlI 2871 CACGTG CAC^GTG
ScaI 208 AGTACT AGT^ACT
SfoI 716 GGCGCC GGC^GCC
SfuI 1457 TTCGAA TT^CGAA
SpeI 4501 ACTAGT A^CTAGT
SspI 3774 AATATT AAT^ATT
StuI 1064 AGGCCT AGG^CCT
SwaI 3968 ATTTAAAT ATTT^AAAT
Tth111I 2002 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1274 CCACCGGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3220 CTCGAG C^TCGAG
XmnI 2198 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 644 GGCGTC G[GA]^CG[CT]C
716 GGCGCC
ApaI 1308 GGGCCC GGGCC^C
2246 GGGCCC
AseI 3462 ATTAAT AT^TAAT
4486 ATTAAT
AsnI 3462 ATTAAT AT^TAAT
4486 ATTAAT
BalI 1535 TGGCCA TGG^CCA
1889 TGGCCA
BclI 1930 TGATCA T^GATCA
2810 TGATCA
BglII 3289 AGATCT A^GATCT
3978 AGATCT
BlpI 234 GCTGAGC GC^T[ACGT]AGC
2473 GCTGAGC
Bpu1102I 234 GCTGAGC GC^T[ACGT]AGC
2473 GCTGAGC
BsaHI 644 GGCGTC G[GA]^CG[CT]C
716 GGCGCC
Bsp1407I 3256 TGTACA T^GTACA
4666 TGTACA
BspHI 2796 TCATGA T^CATGA
3779 TCATGA
BsrGI 3256 TGTACA T^GTACA
4666 TGTACA
BstAPI 1177 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1816 GCAAATTGTGC
CelII 234 GCTGAGC GC^T[ACGT]AGC
2473 GCTGAGC
EcoRI 1466 GAATTC G^AATTC
2315 GAATTC
FbaI 1930 TGATCA T^GATCA
2810 TGATCA
HaeII 716 GGCGCC [GA]GCGC^[CT]
3546 GGCGCT
Hin1I 644 GGCGTC G[GA]^CG[CT]C
716 GGCGCC
HincII 24 GTTAAC GT[CT]^[GA]AC
90 GTCAAC
HindII 24 GTTAAC GT[CT]^[GA]AC
90 GTCAAC
MluNI 1535 TGGCCA TGG^CCA
1889 TGGCCA
MscI 1535 TGGCCA TGG^CCA
1889 TGGCCA
NaeI 1630 GCCGGC GCC^GGC
2426 GCCGGC
NcoI 789 CCATGG C^CATGG
2892 CCATGG
NgoMIV 1630 GCCGGC G^CCGGC
2426 GCCGGC
PpuMI 1057 GGGTCCC [GA]G^G[AT]CC[CT]
2887 GGGACCC
PshBI 3462 ATTAAT AT^TAAT
4486 ATTAAT
PstI 1944 CTGCAG CTGCA^G
2819 CTGCAG
RcaI 2796 TCATGA T^CATGA
3779 TCATGA
SacI 161 GAGCTC GAGCT^C
4423 GAGCTC
SmaI 1305 CCCGGG CCC^GGG
1342 CCCGGG
SspBI 3256 TGTACA T^GTACA
4666 TGTACA
XbaI 2740 TCTAGA T^CTAGA
3629 TCTAGA
XmaCI 1305 CCCGGG C^CCGGG
1342 CCCGGG
XmaI 1305 CCCGGG C^CCGGG
1342 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII