##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AclI AflII AflIII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BfrI BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BstBI ClaI CpoI Eam1105I Eco47III
EcoRI EcoRV FseI FspI HgaI
KasI KpnI KspI MamI MluI
NarI NdeI NotI NruI NspV
PacI PflFI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 2594 TCCGGA T^CCGGA
AgeI 1758 ACCGGT A^CCGGT
BbrPI 1209 CACGTG CAC^GTG
BbsI 1833 GAAGAC GAAGAC(2/6)
BcgI 4206 GCACTAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2180 GTATCC GTATCC(6/5)
BclI 4079 TGATCA T^GATCA
BglI 739 GCCGGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2699 AGATCT A^GATCT
BlpI 2215 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2215 GCTGAGC GC^T[ACGT]AGC
BpuAI 1833 GAAGAC GAAGAC(2/6)
BsaAI 1209 CACGTG [CT]AC^GT[GA]
BseAI 2594 TCCGGA T^CCGGA
BsiEI 737 CGGCCG CG[GA][CT]^CG
BsmBI 1628 CGTCTC CGTCTC(1/5)
BspEI 2594 TCCGGA T^CCGGA
BspHI 3346 TCATGA T^CATGA
BsrBI 801 CCGCTC CCGCTC(-3/-3)
BssSI 2054 CACGAG CACGAG(-5/-1)
Bst1107I 3137 GTATAC GTA^TAC
BstAPI 1664 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3137 GTATAC GTA^TAC
CelII 2215 GCTGAGC GC^T[ACGT]AGC
DrdI 127 GACATTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 737 CGGCCG C^GGCCG
EclXI 737 CGGCCG C^GGCCG
Eco52I 737 CGGCCG C^GGCCG
EcoNI 1580 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2853 ATGCAT ATGCA^T
FbaI 4079 TGATCA T^GATCA
HindIII 3180 AAGCTT A^AGCTT
HpaI 3194 GTTAAC GTT^AAC
MroI 2594 TCCGGA T^CCGGA
NaeI 739 GCCGGC GCC^GGC
NgoMIV 739 GCCGGC G^CCGGC
NheI 1391 GCTAGC G^CTAGC
NsiI 2853 ATGCAT ATGCA^T
NspI 1204 ACATGC [GA]CATG^[CT]
PaeR7I 2616 CTCGAG C^TCGAG
PinAI 1758 ACCGGT A^CCGGT
PmaCI 1209 CACGTG CAC^GTG
PmlI 1209 CACGTG CAC^GTG
RcaI 3346 TCATGA T^CATGA
SexAI 3198 ACCAGGT A^CC[AT]GGT
SfiI 738 GGCCGGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 2617 TCGA T^CGA
TthHB8I 2617 TCGA T^CGA
XbaI 3587 TCTAGA T^CTAGA
XhoI 2616 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 1626 GACGTC GACGT^C
1920 GACGTC
AccI 3137 GTATAC GT^[AC][GT]AC
3567 GTAGAC
AcyI 1626 GACGTC G[GA]^CG[CT]C
1920 GACGTC
AlwNI 1846 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3200 CAGGTCCTG
AocI 4085 CCTGAGG CC^T[ACGT]AGG
4274 CCTGAGG
BanI 1477 GGCACC G^G[CT][GA]CC
2286 GGTGCC
BsaHI 1626 GACGTC G[GA]^CG[CT]C
1920 GACGTC
BsgI 3963 CTGCAC GTGCAG(16/14)
1267 GTGCAG
BstEII 603 GGTGACC G^GT[ACGT]ACC
3546 GGTCACC
BstPI 603 GGTGACC G^GT[ACGT]ACC
3546 GGTCACC
Bsu36I 4085 CCTGAGG CC^T[ACGT]AGG
4274 CCTGAGG
EarI 3422 CTCTTC CTCTTC(1/4)
3432 GAAGAG
Eco81I 4085 CCTGAGG CC^T[ACGT]AGG
4274 CCTGAGG
EcoO65I 603 GGTGACC G^GT[ACGT]ACC
3546 GGTCACC
Hin1I 1626 GACGTC G[GA]^CG[CT]C
1920 GACGTC
Ksp632I 3422 CTCTTC CTCTTC(1/4)
3432 GAAGAG
MfeI 2314 CAATTG C^AATTG
3259 CAATTG
MunI 2314 CAATTG C^AATTG
3259 CAATTG
NcoI 457 CCATGG C^CATGG
2719 CCATGG
SmaI 718 CCCGGG CCC^GGG
3228 CCCGGG
XmaCI 718 CCCGGG C^CCGGG
3228 CCCGGG
XmaI 718 CCCGGG C^CCGGG
3228 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII