################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccI AclI AgeI AhdI AscI AseI AsnI AspEI AspI AviII AvrII BalI BclI BglII BlnI BsaBI BsiWI Bsp1407I BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FbaI FseI FspI MamI MfeI MluI MluNI MscI MunI NcoI NdeI NotI NruI NspI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### Acc65I 3730 GGTACC G^GTACC AccIII 775 TCCGGA T^CCGGA AcyI 1970 GGCGCC G[GA]^CG[CT]C AflII 1677 CTTAAG C^TTAAG AflIII 3579 ACACGT A^C[GA][CT]GT Alw44I 489 GTGCAC G^TGCAC AocI 2013 CCTCAGG CC^T[ACGT]AGG Aor51HI 2631 AGCGCT AGC^GCT ApaI 66 GGGCCC GGGCC^C ApaLI 489 GTGCAC G^TGCAC Asp718I 3730 GGTACC G^GTACC BamHI 2819 GGATCC G^GATCC BbeI 1970 GGCGCC GGCGC^C BbrPI 3580 CACGTG CAC^GTG BcgI 94 CGAGGCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2672 GTATCC GTATCC(6/5) BfrI 1677 CTTAAG C^TTAAG BsaAI 3580 CACGTG [CT]AC^GT[GA] BsaHI 1970 GGCGCC G[GA]^CG[CT]C BseAI 775 TCCGGA T^CCGGA BsgI 1923 GTGCAG GTGCAG(16/14) BsiEI 3097 CGGTCG CG[GA][CT]^CG BspDI 225 ATCGAT AT^CGAT BspEI 775 TCCGGA T^CCGGA BsrBI 1471 CCGCTC CCGCTC(-3/-3) BsrDI 1151 CATTGC GCAATG(2/0) BssSI 3691 CTCGTG CACGAG(-5/-1) BstYI 2819 GGATCC [GA]^GATC[CT] Bsu36I 2013 CCTCAGG CC^T[ACGT]AGG ClaI 225 ATCGAT AT^CGAT CpoI 115 CGGTCCG CG^G[AT]CCG DraIII 3997 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2631 AGCGCT AGC^GCT Eco81I 2013 CCTCAGG CC^T[ACGT]AGG EcoRI 1903 GAATTC G^AATTC EcoT22I 1203 ATGCAT ATGCA^T Hin1I 1970 GGCGCC G[GA]^CG[CT]C HincII 4096 GTTAAC GT[CT]^[GA]AC HindII 4096 GTTAAC GT[CT]^[GA]AC HindIII 2933 AAGCTT A^AGCTT HpaI 4096 GTTAAC GTT^AAC KasI 1970 GGCGCC G^GCGCC KpnI 3730 GGTACC GGTAC^C KspI 2187 CCGCGG CCGC^GG MflI 2819 GGATCC [GA]^GATC[CT] MroI 775 TCCGGA T^CCGGA NaeI 1973 GCCGGC GCC^GGC NarI 1970 GGCGCC GG^CGCC NgoMIV 1973 GCCGGC G^CCGGC NheI 2001 GCTAGC G^CTAGC NsiI 1203 ATGCAT ATGCA^T PaeR7I 3116 CTCGAG C^TCGAG PmaCI 3580 CACGTG CAC^GTG PmlI 3580 CACGTG CAC^GTG PshAI 3159 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 115 CGGTCCG CG^G[AT]CCG SacII 2187 CCGCGG CCGC^GG SapI 3675 GCTCTTC GCTCTTC(1/4) SfoI 1970 GGCGCC GGC^GCC SgrAI 1972 CGCCGGCG C[GA]^CCGG[CT]G SmaI 2497 CCCGGG CCC^GGG SspI 4069 AATATT AAT^ATT XbaI 1907 TCTAGA T^CTAGA XhoI 3116 CTCGAG C^TCGAG XhoII 2819 GGATCC [GA]^GATC[CT] XmaCI 2497 CCCGGG C^CCGGG XmaI 2497 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Asp700I 1571 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3148 GAAGACATTC BglI 1479 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1755 GCCATGCAGGC BlpI 1155 GCTGAGC GC^T[ACGT]AGC 3277 GCTGAGC Bpu1102I 1155 GCTGAGC GC^T[ACGT]AGC 3277 GCTGAGC BsmBI 2045 CGTCTC CGTCTC(1/5) 1514 GAGACG BstAPI 968 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2201 GCAGCAGCTGC BstXI 1540 CCAATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2975 CCACCCAACTGG CelII 1155 GCTGAGC GC^T[ACGT]AGC 3277 GCTGAGC EaeI 407 TGGCCG [CT]^GGCC[GA] 1127 TGGCCG MaeII 1258 ACGT A^CGT 3581 ACGT PflMI 1278 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3996 CCACCATGTGG PpuMI 1645 GGGACCT [GA]G^G[AT]CC[CT] 1993 AGGACCT StuI 1264 AGGCCT AGG^CCT 2266 AGGCCT Van91I 1278 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3996 CCACCATGTGG XmnI 1571 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3148 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI