##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       AccI        AclI        AgeI        AhdI        
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BalI        BclI        BglII       
BlnI        BsaBI       BsiWI       Bsp1407I    BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
FbaI        FseI        FspI        MamI        MfeI        
MluI        MluNI       MscI        MunI        NcoI        
NdeI        NotI        NruI        NspI        NspV        
PacI        PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Tth111I     


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

Acc65I       3730  GGTACC               G^GTACC
AccIII        775  TCCGGA               T^CCGGA
AcyI         1970  GGCGCC               G[GA]^CG[CT]C
AflII        1677  CTTAAG               C^TTAAG
AflIII       3579  ACACGT               A^C[GA][CT]GT
Alw44I        489  GTGCAC               G^TGCAC
AocI         2013  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2631  AGCGCT               AGC^GCT
ApaI           66  GGGCCC               GGGCC^C
ApaLI         489  GTGCAC               G^TGCAC
Asp718I      3730  GGTACC               G^GTACC
BamHI        2819  GGATCC               G^GATCC
BbeI         1970  GGCGCC               GGCGC^C
BbrPI        3580  CACGTG               CAC^GTG
BcgI           94  CGAGGCACTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2672  GTATCC               GTATCC(6/5)
BfrI         1677  CTTAAG               C^TTAAG
BsaAI        3580  CACGTG               [CT]AC^GT[GA]
BsaHI        1970  GGCGCC               G[GA]^CG[CT]C
BseAI         775  TCCGGA               T^CCGGA
BsgI         1923  GTGCAG               GTGCAG(16/14)
BsiEI        3097  CGGTCG               CG[GA][CT]^CG
BspDI         225  ATCGAT               AT^CGAT
BspEI         775  TCCGGA               T^CCGGA
BsrBI        1471  CCGCTC               CCGCTC(-3/-3)
BsrDI        1151  CATTGC               GCAATG(2/0)
BssSI        3691  CTCGTG               CACGAG(-5/-1)
BstYI        2819  GGATCC               [GA]^GATC[CT]
Bsu36I       2013  CCTCAGG              CC^T[ACGT]AGG
ClaI          225  ATCGAT               AT^CGAT
CpoI          115  CGGTCCG              CG^G[AT]CCG
DraIII       3997  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2631  AGCGCT               AGC^GCT
Eco81I       2013  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1903  GAATTC               G^AATTC
EcoT22I      1203  ATGCAT               ATGCA^T
Hin1I        1970  GGCGCC               G[GA]^CG[CT]C
HincII       4096  GTTAAC               GT[CT]^[GA]AC
HindII       4096  GTTAAC               GT[CT]^[GA]AC
HindIII      2933  AAGCTT               A^AGCTT
HpaI         4096  GTTAAC               GTT^AAC
KasI         1970  GGCGCC               G^GCGCC
KpnI         3730  GGTACC               GGTAC^C
KspI         2187  CCGCGG               CCGC^GG
MflI         2819  GGATCC               [GA]^GATC[CT]
MroI          775  TCCGGA               T^CCGGA
NaeI         1973  GCCGGC               GCC^GGC
NarI         1970  GGCGCC               GG^CGCC
NgoMIV       1973  GCCGGC               G^CCGGC
NheI         2001  GCTAGC               G^CTAGC
NsiI         1203  ATGCAT               ATGCA^T
PaeR7I       3116  CTCGAG               C^TCGAG
PmaCI        3580  CACGTG               CAC^GTG
PmlI         3580  CACGTG               CAC^GTG
PshAI        3159  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII         115  CGGTCCG              CG^G[AT]CCG
SacII        2187  CCGCGG               CCGC^GG
SapI         3675  GCTCTTC              GCTCTTC(1/4)
SfoI         1970  GGCGCC               GGC^GCC
SgrAI        1972  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         2497  CCCGGG               CCC^GGG
SspI         4069  AATATT               AAT^ATT
XbaI         1907  TCTAGA               T^CTAGA
XhoI         3116  CTCGAG               C^TCGAG
XhoII        2819  GGATCC               [GA]^GATC[CT]
XmaCI        2497  CCCGGG               C^CCGGG
XmaI         2497  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Asp700I      1571  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3148  GAAGACATTC          
BglI         1479  GCCTTTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1755  GCCATGCAGGC         
BlpI         1155  GCTGAGC              GC^T[ACGT]AGC
             3277  GCTGAGC             
Bpu1102I     1155  GCTGAGC              GC^T[ACGT]AGC
             3277  GCTGAGC             
BsmBI        2045  CGTCTC               CGTCTC(1/5)
             1514  GAGACG              
BstAPI        968  GCAGGTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2201  GCAGCAGCTGC         
BstXI        1540  CCAATCAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2975  CCACCCAACTGG        
CelII        1155  GCTGAGC              GC^T[ACGT]AGC
             3277  GCTGAGC             
EaeI          407  TGGCCG               [CT]^GGCC[GA]
             1127  TGGCCG              
MaeII        1258  ACGT                 A^CGT
             3581  ACGT                
PflMI        1278  CCAGCATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3996  CCACCATGTGG         
PpuMI        1645  GGGACCT              [GA]G^G[AT]CC[CT]
             1993  AGGACCT             
StuI         1264  AGGCCT               AGG^CCT
             2266  AGGCCT              
Van91I       1278  CCAGCATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3996  CCACCATGTGG         
XmnI         1571  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3148  GAAGACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI