##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BglI        BlnI        BsaHI       BsaWI       BseAI       
BsiWI       BsmBI       BspEI       BspHI       BsrFI       
BssHII      BstBI       BstUI       BstXI       CfoI        
Cfr10I      CpoI        Eam1105I    Eco47III    EcoNI       
EcoRI       FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
KasI        KpnI        KspI        MluI        MluNI       
MroI        MscI        MspI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RcaI        RsrII       SacI        SacII       SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       Sse8387I    SwaI        
Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AocI         3375  CCTCAGG              CC^T[ACGT]AGG
AspHI         137  GAGCAC               G[AT]GC[AT]^C
BciVI        1143  GTATCC               GTATCC(6/5)
BglII         928  AGATCT               A^GATCT
BlpI          629  GCTCAGC              GC^T[ACGT]AGC
BmyI          137  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      629  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2671  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1582  CGGCCG               CG[GA][CT]^CG
BsiHKAI       137  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      137  GAGCAC               G[AGT]GC[ACT]^C
BsrBI        3285  GAGCGG               CCGCTC(-3/-3)
BssSI        3066  CACGAG               CACGAG(-5/-1)
Bst1107I     3321  GTATAC               GTA^TAC
BstAPI        959  GCAACTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      3321  GTATAC               GTA^TAC
Bsu36I       3375  CCTCAGG              CC^T[ACGT]AGG
CelII         629  GCTCAGC              GC^T[ACGT]AGC
DraIII       2226  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2776  GACACCCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1582  CGGCCG               [CT]^GGCC[GA]
EagI         1582  CGGCCG               C^GGCCG
EclXI        1582  CGGCCG               C^GGCCG
Eco52I       1582  CGGCCG               C^GGCCG
Eco81I       3375  CCTCAGG              CC^T[ACGT]AGG
FauI         3287  GCGGG                CCCGC(4/6)
HpaI         2155  GTTAAC               GTT^AAC
MamI         2671  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1536  CAATTG               C^AATTG
MspA1I        611  CAGCTG               C[AC]G^C[GT]G
MunI         1536  CAATTG               C^AATTG
PaeR7I        635  CTCGAG               C^TCGAG
PvuII         611  CAGCTG               CAG^CTG
SalI         2999  GTCGAC               G^TCGAC
SpeI         2326  ACTAGT               A^CTAGT
SphI         3744  GCATGC               GCATG^C
StuI         1408  AGGCCT               AGG^CCT
XcmI         4061  CCAGGGTGTATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          635  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AciI         1581  GCGG                 CCGC(-3/-1)
             3287  GCGG                
AclI           98  AACGTT               AA^CGTT
             2358  AACGTT              
Asp700I      2285  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2660  GAAAAAATTC          
AvaI          635  CTCGAG               C^[CT]CG[GA]G
             1872  CCCGAG              
BanI         1184  GGCACC               G^G[CT][GA]CC
             2183  GGCACC              
BbsI         2249  GAAGAC               GAAGAC(2/6)
             2760  GTCTTC              
BclI         1978  TGATCA               T^GATCA
             4401  TGATCA              
BpuAI        2249  GAAGAC               GAAGAC(2/6)
             2760  GTCTTC              
BsaI          885  GAGACC               GGTCTC(1/5)
             3292  GAGACC              
BsgI         1137  CTGCAC               GTGCAG(16/14)
              620  GTGCAG              
BsmI         3588  GAATGC               GAATGC(1/-1)
             4432  GCATTC              
BsoBI         635  CTCGAG               C^[CT]CG[GA]G
             1872  CCCGAG              
BspDI        2019  ATCGAT               AT^CGAT
             3050  ATCGAT              
BspLU11I     2589  ACATGT               A^CATGT
             3012  ACATGT              
BspMI        1863  ACCTGC               ACCTGC(4/8)
             1884  ACCTGC              
BstEII       3871  GGTGACC              G^GT[ACGT]ACC
             4075  GGTAACC             
BstPI        3871  GGTGACC              G^GT[ACGT]ACC
             4075  GGTAACC             
ClaI         2019  ATCGAT               AT^CGAT
             3050  ATCGAT              
EcoO65I      3871  GGTGACC              G^GT[ACGT]ACC
             4075  GGTAACC             
EcoRV        1127  GATATC               GAT^ATC
             2396  GATATC              
EcoT14I      1674  CCATGG               C^C[AT][AT]GG
             2712  CCATGG              
FbaI         1978  TGATCA               T^GATCA
             4401  TGATCA              
HincII       2155  GTTAAC               GT[CT]^[GA]AC
             2999  GTCGAC              
HindII       2155  GTTAAC               GT[CT]^[GA]AC
             2999  GTCGAC              
HindIII       269  AAGCTT               A^AGCTT
             2524  AAGCTT              
NcoI         1674  CCATGG               C^CATGG
             2712  CCATGG              
PleI         1226  GACTC                GAGTC(4/5)
             2036  GAGTC               
Psp1406I       98  AACGTT               AA^CGTT
             2358  AACGTT              
StyI         1674  CCATGG               C^C[AT][AT]GG
             2712  CCATGG              
XbaI          931  TCTAGA               T^CTAGA
             4196  TCTAGA              
XmnI         2285  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2660  GAAAAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccI        AcsI        AfaI        AflII       AflIII      
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        AvaII       BbvI        BfaI        BfrI        
BpmI        BsaAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1407I    BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT22I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PflMI       PpuMI       PshBI       PstI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII