##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
AscI AspEI AspI AvrII BbrPI
BlnI BsaAI BsiWI BspDI BsrBI
BsrFI BssHII BssSI BstEII BstPI
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI Eam1105I Eco81I EcoO65I FseI
KspI MluI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SgrAI SnaBI Sse8387I
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 75 enzymes) #
###################################
Acc65I 3655 GGTACC G^GTACC
AccI 2951 GTATAC GT^[AC][GT]AC
AccII 6 CGCG CG^CG
AccIII 1373 TCCGGA T^CCGGA
AflII 4039 CTTAAG C^TTAAG
AflIII 1623 ACATGT A^C[GA][CT]GT
Alw44I 3520 GTGCAC G^TGCAC
Aor51HI 2332 AGCGCT AGC^GCT
ApaI 16 GGGCCC GGGCC^C
ApaLI 3520 GTGCAC G^TGCAC
Asp700I 205 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3655 GGTACC G^GTACC
AviII 3443 TGCGCA TGC^GCA
BalI 1443 TGGCCA TGG^CCA
BbeI 1 GGCGCC GGCGC^C
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BcgI 3914 GCATGTCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1425 GGATAC GTATCC(6/5)
BclI 466 TGATCA T^GATCA
BfrI 4039 CTTAAG C^TTAAG
BglI 3081 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2050 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2050 GCTCAGC GC^T[ACGT]AGC
BsaBI 876 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1373 TCCGGA [AT]^CCGG[AT]
BseAI 1373 TCCGGA T^CCGGA
BsiEI 29 CGGCCG CG[GA][CT]^CG
BsmBI 3233 CGTCTC CGTCTC(1/5)
BsmI 1960 GAATGC GAATGC(1/-1)
BspEI 1373 TCCGGA T^CCGGA
BspLU11I 1623 ACATGT A^CATGT
BspMI 1050 ACCTGC ACCTGC(4/8)
Bst1107I 2951 GTATAC GTA^TAC
BstBI 2470 TTCGAA TT^CGAA
BstUI 6 CGCG CG^CG
BstZ17I 2951 GTATAC GTA^TAC
CelII 2050 GCTCAGC GC^T[ACGT]AGC
EagI 29 CGGCCG C^GGCCG
EclXI 29 CGGCCG C^GGCCG
Eco47III 2332 AGCGCT AGC^GCT
Eco52I 29 CGGCCG C^GGCCG
EcoNI 3456 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4265 GAATTC G^AATTC
EcoRV 1972 GATATC GAT^ATC
EcoT22I 1134 ATGCAT ATGCA^T
FbaI 466 TGATCA T^GATCA
FspI 3443 TGCGCA TGC^GCA
HgaI 87 GCGTC GACGC(5/10)
HincII 3257 GTTAAC GT[CT]^[GA]AC
HindII 3257 GTTAAC GT[CT]^[GA]AC
HpaI 3257 GTTAAC GTT^AAC
KasI 1 GGCGCC G^GCGCC
KpnI 3655 GGTACC GGTAC^C
MaeII 2411 ACGT A^CGT
MamI 876 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1443 TGGCCA TGG^CCA
MroI 1373 TCCGGA T^CCGGA
MscI 1443 TGGCCA TGG^CCA
MvnI 6 CGCG CG^CG
NarI 1 GGCGCC GG^CGCC
NcoI 1318 CCATGG C^CATGG
NsiI 1134 ATGCAT ATGCA^T
NspV 2470 TTCGAA TT^CGAA
PpuMI 2450 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 442 CAGCTG CAG^CTG
SacI 3399 GAGCTC GAGCT^C
SapI 3396 GAAGAGC GCTCTTC(1/4)
SfoI 1 GGCGCC GGC^GCC
SfuI 2470 TTCGAA TT^CGAA
SmaI 64 CCCGGG CCC^GGG
SpeI 2034 ACTAGT A^CTAGT
XbaI 2553 TCTAGA T^CTAGA
XmaCI 64 CCCGGG C^CCGGG
XmaI 64 CCCGGG C^CCGGG
XmnI 205 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1 GGCGCC G[GA]^CG[CT]C
86 GGCGTC
AlwNI 1557 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
3077 CAGTGCCTG
AvaI 64 CCCGGG C^[CT]CG[GA]G
396 CTCGGG
BamHI 2269 GGATCC G^GATCC
2858 GGATCC
BglII 1259 AGATCT A^GATCT
1841 AGATCT
BsaHI 1 GGCGCC G[GA]^CG[CT]C
86 GGCGTC
BsaI 401 GAGACC GGTCTC(1/5)
2185 GGTCTC
BsoBI 64 CCCGGG C^[CT]CG[GA]G
396 CTCGGG
BsrDI 2210 CATTGC GCAATG(2/0)
2083 GCAATG
BstXI 911 CCATTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2357 CCATGTAAATGG
DraI 102 TTTAAA TTT^AAA
4129 TTTAAA
DraII 15 AGGGCCC [GA]G^G[ACGT]CC[CT]
2450 GGGTCCT
DsaI 1318 CCATGG C^C[GA][CT]GG
2181 CCACGG
EarI 3396 GAAGAG CTCTTC(1/4)
3567 GAAGAG
EcoO109I 15 AGGGCCC [GA]G^G[ACGT]CC[CT]
2450 GGGTCCT
Hin1I 1 GGCGCC G[GA]^CG[CT]C
86 GGCGTC
HindIII 1105 AAGCTT A^AGCTT
3412 AAGCTT
Ksp632I 3396 GAAGAG CTCTTC(1/4)
3567 GAAGAG
MspA1I 442 CAGCTG C[AC]G^C[GT]G
3252 CAGCGG
PflMI 1446 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3834 CCAAAGGCTGG
SexAI 404 ACCTGGT A^CC[AT]GGT
3461 ACCAGGT
SphI 2227 GCATGC GCATG^C
3090 GCATGC
Tsp45I 2161 GTGAC ^GT[GC]AC
3683 GTGAC
Van91I 1446 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3834 CCAAAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspI MunI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI PstI RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI XhoII