##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BbeI BcgI BciVI
BglI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BstAPI BstEII BstPI CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 292 CGCG CG^CG
AocI 1621 CCTTAGG CC^T[ACGT]AGG
ApaI 1279 GGGCCC GGGCC^C
AvaI 397 CTCGGG C^[CT]CG[GA]G
AvrII 3940 CCTAGG C^CTAGG
BalI 449 TGGCCA TGG^CCA
BanI 507 GGTGCC G^G[CT][GA]CC
BclI 4070 TGATCA T^GATCA
BlnI 3940 CCTAGG C^CTAGG
BsmI 3184 GAATGC GAATGC(1/-1)
BsoBI 397 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3672 TGTACA T^GTACA
BspMI 3806 ACCTGC ACCTGC(4/8)
BsrDI 2891 GCAATG GCAATG(2/0)
BsrGI 3672 TGTACA T^GTACA
BssSI 28 CACGAG CACGAG(-5/-1)
Bst1107I 2837 GTATAC GTA^TAC
BstUI 292 CGCG CG^CG
BstZ17I 2837 GTATAC GTA^TAC
Bsu36I 1621 CCTTAGG CC^T[ACGT]AGG
EaeI 449 TGGCCA [CT]^GGCC[GA]
Eco81I 1621 CCTTAGG CC^T[ACGT]AGG
EcoNI 1431 CCTTATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2725 GAATTC G^AATTC
FauI 1507 CCCGC CCCGC(4/6)
FbaI 4070 TGATCA T^GATCA
HindIII 3424 AAGCTT A^AGCTT
MluNI 449 TGGCCA TGG^CCA
MscI 449 TGGCCA TGG^CCA
MspA1I 502 CAGCTG C[AC]G^C[GT]G
MvnI 292 CGCG CG^CG
NheI 3810 GCTAGC G^CTAGC
PacI 2381 TTAATTAA TTAAT^TAA
PflMI 629 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2045 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 502 CAGCTG CAG^CTG
SapI 938 GAAGAGC GCTCTTC(1/4)
ScaI 544 AGTACT AGT^ACT
SnaBI 2840 TACGTA TAC^GTA
SspBI 3672 TGTACA T^GTACA
Van91I 629 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1561 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 572 GTCTAC GT^[AC][GT]AC
2837 GTATAC
AciI 1244 CCGC CCGC(-3/-1)
1508 CCGC
Asp700I 938 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2805 GAAAGTCTTC
BamHI 191 GGATCC G^GATCC
1848 GGATCC
BbrPI 3216 CACGTG CAC^GTG
3999 CACGTG
BcnI 841 CCCGG CC^[GC]GG
3854 CCGGG
BsgI 4081 CTGCAC GTGCAG(16/14)
1222 GTGCAG
BsmBI 31 GAGACG CGTCTC(1/5)
621 GAGACG
BspLU11I 1885 ACATGT A^CATGT
3788 ACATGT
BstBI 1198 TTCGAA TT^CGAA
3623 TTCGAA
Cac8I 606 GCAGGC GC[ACGT]^[ACGT]GC
3810 GCTAGC
DsaI 187 CCATGG C^C[GA][CT]GG
958 CCATGG
HapII 842 CCGG C^CGG
3854 CCGG
HpaII 842 CCGG C^CGG
3854 CCGG
MspI 842 CCGG C^CGG
3854 CCGG
NciI 841 CCCGG CC^[GC]GG
3854 CCGGG
NcoI 187 CCATGG C^CATGG
958 CCATGG
NspV 1198 TTCGAA TT^CGAA
3623 TTCGAA
PmaCI 3216 CACGTG CAC^GTG
3999 CACGTG
PmlI 3216 CACGTG CAC^GTG
3999 CACGTG
PpuMI 1002 AGGTCCC [GA]G^G[AT]CC[CT]
3556 AGGACCT
SfuI 1198 TTCGAA TT^CGAA
3623 TTCGAA
XmnI 938 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2805 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AcsI AfaI AflII AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvCI BbvI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII