##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AscI        
AseI        AsnI        Asp718I     AspI        AvrII       
BbeI        BbvCI       BciVI       BfrI        BlnI        
BsiWI       BssHII      Bst1107I    BstBI       BstZ17I     
CpoI        DraI        EagI        EclXI       Eco52I      
EcoRI       EcoRV       EcoT22I     FseI        HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MunI        NarI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshBI       Psp1406I    RsrII       
SacII       SalI        SapI        SbfI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccIII       2661  TCCGGA               T^CCGGA
AcsI         1006  AAATTC               [GA]^AATT[CT]
AocI         2386  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       342  AGCGCT               AGC^GCT
ApoI         1006  AAATTC               [GA]^AATT[CT]
BclI         3601  TGATCA               T^GATCA
BglII        3843  AGATCT               A^GATCT
BsaBI        2734  GATGGCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2661  TCCGGA               T^CCGGA
BsmBI        3510  GAGACG               CGTCTC(1/5)
BspEI        2661  TCCGGA               T^CCGGA
BspHI        2790  TCATGA               T^CATGA
BspLU11I     2718  ACATGT               A^CATGT
BssSI        1772  CTCGTG               CACGAG(-5/-1)
Bsu36I       2386  CCTGAGG              CC^T[ACGT]AGG
DraIII       2570  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      342  AGCGCT               AGC^GCT
Eco81I       2386  CCTGAGG              CC^T[ACGT]AGG
FbaI         3601  TGATCA               T^GATCA
HindIII        84  AAGCTT               A^AGCTT
MamI         2734  GATGGCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2661  TCCGGA               T^CCGGA
PshAI        4072  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         1821  CGATCG               CGAT^CG
RcaI         2790  TCATGA               T^CATGA
SacI         3396  GAGCTC               GAGCT^C
ScaI         2901  AGTACT               AGT^ACT
SfiI         3962  GGCCAAACAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          678  CCCGGG               CCC^GGG
Tsp509I      1007  AATT                 ^AATT
XmaCI         678  CCCGGG               C^CCGGG
XmaI          678  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          698  GTCTAC               GT^[AC][GT]AC
             1439  GTCTAC              
AflIII       1428  ACGTGT               A^C[GA][CT]GT
             2718  ACATGT              
AhdI          662  GACAACGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1574  GACCGCGAGTC         
Alw44I        629  GTGCAC               G^TGCAC
             3426  GTGCAC              
ApaLI         629  GTGCAC               G^TGCAC
             3426  GTGCAC              
Asp700I      1858  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2653  GAATCTTTTC          
AspEI         662  GACAACGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1574  GACCGCGAGTC         
AviII        2277  TGCGCA               TGC^GCA
             2548  TGCGCA              
BfaI         2042  CTAG                 C^TAG
             3639  CTAG                
Bsp1407I     1593  TGTACA               T^GTACA
             2812  TGTACA              
BspDI        1679  ATCGAT               AT^CGAT
             2627  ATCGAT              
BsrBI        3500  CCGCTC               CCGCTC(-3/-3)
              443  GAGCGG              
BsrGI        1593  TGTACA               T^GTACA
             2812  TGTACA              
BstEII        526  GGTCACC              G^GT[ACGT]ACC
             1228  GGTGACC             
BstPI         526  GGTCACC              G^GT[ACGT]ACC
             1228  GGTGACC             
ClaI         1679  ATCGAT               AT^CGAT
             2627  ATCGAT              
DrdI         2396  GACCAGCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4151  GACAAGAAAGTC        
Eam1105I      662  GACAACGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1574  GACCGCGAGTC         
EarI         2291  CTCTTC               CTCTTC(1/4)
             1853  GAAGAG              
EcoO65I       526  GGTCACC              G^GT[ACGT]ACC
             1228  GGTGACC             
FspI         2277  TGCGCA               TGC^GCA
             2548  TGCGCA              
HaeII         342  AGCGCT               [GA]GCGC^[CT]
             1566  AGCGCC              
HincII        377  GTCAAC               GT[CT]^[GA]AC
             1165  GTTGAC              
HindII        377  GTCAAC               GT[CT]^[GA]AC
             1165  GTTGAC              
Ksp632I      2291  CTCTTC               CTCTTC(1/4)
             1853  GAAGAG              
MaeI         2042  CTAG                 C^TAG
             3639  CTAG                
NcoI         2253  CCATGG               C^CATGG
             3043  CCATGG              
NruI         1000  TCGCGA               TCG^CGA
             3593  TCGCGA              
SexAI         546  ACCTGGT              A^CC[AT]GGT
             2001  ACCTGGT             
SspBI        1593  TGTACA               T^GTACA
             2812  TGTACA              
XmnI         1858  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2653  GAATCTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbrPI       BbsI        BbvI        BcgI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        XhoII