################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AspI AvrII BbeI BbvCI BciVI BfrI BlnI BsiWI BssHII Bst1107I BstBI BstZ17I CpoI DraI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FseI HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I RsrII SacII SalI SapI SbfI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 2661 TCCGGA T^CCGGA AcsI 1006 AAATTC [GA]^AATT[CT] AocI 2386 CCTGAGG CC^T[ACGT]AGG Aor51HI 342 AGCGCT AGC^GCT ApoI 1006 AAATTC [GA]^AATT[CT] BclI 3601 TGATCA T^GATCA BglII 3843 AGATCT A^GATCT BsaBI 2734 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2661 TCCGGA T^CCGGA BsmBI 3510 GAGACG CGTCTC(1/5) BspEI 2661 TCCGGA T^CCGGA BspHI 2790 TCATGA T^CATGA BspLU11I 2718 ACATGT A^CATGT BssSI 1772 CTCGTG CACGAG(-5/-1) Bsu36I 2386 CCTGAGG CC^T[ACGT]AGG DraIII 2570 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 342 AGCGCT AGC^GCT Eco81I 2386 CCTGAGG CC^T[ACGT]AGG FbaI 3601 TGATCA T^GATCA HindIII 84 AAGCTT A^AGCTT MamI 2734 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2661 TCCGGA T^CCGGA PshAI 4072 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1821 CGATCG CGAT^CG RcaI 2790 TCATGA T^CATGA SacI 3396 GAGCTC GAGCT^C ScaI 2901 AGTACT AGT^ACT SfiI 3962 GGCCAAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 678 CCCGGG CCC^GGG Tsp509I 1007 AATT ^AATT XmaCI 678 CCCGGG C^CCGGG XmaI 678 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 698 GTCTAC GT^[AC][GT]AC 1439 GTCTAC AflIII 1428 ACGTGT A^C[GA][CT]GT 2718 ACATGT AhdI 662 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1574 GACCGCGAGTC Alw44I 629 GTGCAC G^TGCAC 3426 GTGCAC ApaLI 629 GTGCAC G^TGCAC 3426 GTGCAC Asp700I 1858 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2653 GAATCTTTTC AspEI 662 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1574 GACCGCGAGTC AviII 2277 TGCGCA TGC^GCA 2548 TGCGCA BfaI 2042 CTAG C^TAG 3639 CTAG Bsp1407I 1593 TGTACA T^GTACA 2812 TGTACA BspDI 1679 ATCGAT AT^CGAT 2627 ATCGAT BsrBI 3500 CCGCTC CCGCTC(-3/-3) 443 GAGCGG BsrGI 1593 TGTACA T^GTACA 2812 TGTACA BstEII 526 GGTCACC G^GT[ACGT]ACC 1228 GGTGACC BstPI 526 GGTCACC G^GT[ACGT]ACC 1228 GGTGACC ClaI 1679 ATCGAT AT^CGAT 2627 ATCGAT DrdI 2396 GACCAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4151 GACAAGAAAGTC Eam1105I 662 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1574 GACCGCGAGTC EarI 2291 CTCTTC CTCTTC(1/4) 1853 GAAGAG EcoO65I 526 GGTCACC G^GT[ACGT]ACC 1228 GGTGACC FspI 2277 TGCGCA TGC^GCA 2548 TGCGCA HaeII 342 AGCGCT [GA]GCGC^[CT] 1566 AGCGCC HincII 377 GTCAAC GT[CT]^[GA]AC 1165 GTTGAC HindII 377 GTCAAC GT[CT]^[GA]AC 1165 GTTGAC Ksp632I 2291 CTCTTC CTCTTC(1/4) 1853 GAAGAG MaeI 2042 CTAG C^TAG 3639 CTAG NcoI 2253 CCATGG C^CATGG 3043 CCATGG NruI 1000 TCGCGA TCG^CGA 3593 TCGCGA SexAI 546 ACCTGGT A^CC[AT]GGT 2001 ACCTGGT SspBI 1593 TGTACA T^GTACA 2812 TGTACA XmnI 1858 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2653 GAATCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvI BcgI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII