################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI ApoI AscI Asp718I AspI AviII AvrII BbeI BfrI BlnI BlpI Bpu1102I BsaWI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstZ17I CelII ClaI DraIII EcoRI EcoRV FseI FspI HpaI KasI KpnI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI Psp1406I PvuI SalI SbfI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 761 GACGTC GACGT^C AhdI 3647 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 476 AGCGCT AGC^GCT AseI 1003 ATTAAT AT^TAAT AsnI 1003 ATTAAT AT^TAAT AspEI 3647 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 3517 CACGTG CAC^GTG BciVI 1220 GTATCC GTATCC(6/5) BclI 2580 TGATCA T^GATCA BglII 3698 AGATCT A^GATCT BsaBI 661 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 51 TGTACA T^GTACA BspHI 2577 TCATGA T^CATGA BsrGI 51 TGTACA T^GTACA BstAPI 539 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 400 GGTCACC G^GT[ACGT]ACC BstPI 400 GGTCACC G^GT[ACGT]ACC CpoI 857 CGGACCG CG^G[AT]CCG DraI 2966 TTTAAA TTT^AAA DrdI 3286 GACATGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3647 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 476 AGCGCT AGC^GCT EcoO65I 400 GGTCACC G^GT[ACGT]ACC EcoT22I 2703 ATGCAT ATGCA^T FbaI 2580 TGATCA T^GATCA HaeII 476 AGCGCT [GA]GCGC^[CT] HincII 65 GTTGAC GT[CT]^[GA]AC HindII 65 GTTGAC GT[CT]^[GA]AC HindIII 1810 AAGCTT A^AGCTT MamI 661 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NsiI 2703 ATGCAT ATGCA^T NspI 1105 GCATGT [GA]CATG^[CT] PaeR7I 2643 CTCGAG C^TCGAG PmaCI 3517 CACGTG CAC^GTG PmlI 3517 CACGTG CAC^GTG PshBI 1003 ATTAAT AT^TAAT RcaI 2577 TCATGA T^CATGA RsrII 857 CGGACCG CG^G[AT]CCG SapI 2548 GCTCTTC GCTCTTC(1/4) ScaI 2028 AGTACT AGT^ACT SexAI 2529 ACCAGGT A^CC[AT]GGT SfiI 1282 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 51 TGTACA T^GTACA SspI 1000 AATATT AAT^ATT Tsp509I 4120 AATT ^AATT XhoI 2643 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AflIII 30 ACGTGT A^C[GA][CT]GT 3518 ACGTGT Alw44I 1882 GTGCAC G^TGCAC 2816 GTGCAC ApaLI 1882 GTGCAC G^TGCAC 2816 GTGCAC BcgI 1162 CGAAGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2808 CGAGCCTCGTGC BsaAI 29 TACGTG [CT]AC^GT[GA] 3517 CACGTG BsmI 2303 GCATTC GAATGC(1/-1) 2845 GCATTC BstXI 1563 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3733 CCACTTTCATGG KspI 247 CCGCGG CCGC^GG 2370 CCGCGG NaeI 1957 GCCGGC GCC^GGC 3781 GCCGGC NgoMIV 1957 GCCGGC G^CCGGC 3781 GCCGGC SacI 1633 GAGCTC GAGCT^C 3396 GAGCTC SacII 247 CCGCGG CCGC^GG 2370 CCGCGG ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI