##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        ApoI        AscI        Asp718I     
AspI        AviII       AvrII       BbeI        BfrI        
BlnI        BlpI        Bpu1102I    BsaWI       BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BsrBI       
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
ClaI        DraIII      EcoRI       EcoRV       FseI        
FspI        HpaI        KasI        KpnI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PflFI       PinAI       PmeI        Psp1406I    PvuI        
SalI        SbfI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SwaI        
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         761  GACGTC               GACGT^C
AhdI         3647  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       476  AGCGCT               AGC^GCT
AseI         1003  ATTAAT               AT^TAAT
AsnI         1003  ATTAAT               AT^TAAT
AspEI        3647  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        3517  CACGTG               CAC^GTG
BciVI        1220  GTATCC               GTATCC(6/5)
BclI         2580  TGATCA               T^GATCA
BglII        3698  AGATCT               A^GATCT
BsaBI         661  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I       51  TGTACA               T^GTACA
BspHI        2577  TCATGA               T^CATGA
BsrGI          51  TGTACA               T^GTACA
BstAPI        539  GCAGTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        400  GGTCACC              G^GT[ACGT]ACC
BstPI         400  GGTCACC              G^GT[ACGT]ACC
CpoI          857  CGGACCG              CG^G[AT]CCG
DraI         2966  TTTAAA               TTT^AAA
DrdI         3286  GACATGAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3647  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      476  AGCGCT               AGC^GCT
EcoO65I       400  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2703  ATGCAT               ATGCA^T
FbaI         2580  TGATCA               T^GATCA
HaeII         476  AGCGCT               [GA]GCGC^[CT]
HincII         65  GTTGAC               GT[CT]^[GA]AC
HindII         65  GTTGAC               GT[CT]^[GA]AC
HindIII      1810  AAGCTT               A^AGCTT
MamI          661  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI         2703  ATGCAT               ATGCA^T
NspI         1105  GCATGT               [GA]CATG^[CT]
PaeR7I       2643  CTCGAG               C^TCGAG
PmaCI        3517  CACGTG               CAC^GTG
PmlI         3517  CACGTG               CAC^GTG
PshBI        1003  ATTAAT               AT^TAAT
RcaI         2577  TCATGA               T^CATGA
RsrII         857  CGGACCG              CG^G[AT]CCG
SapI         2548  GCTCTTC              GCTCTTC(1/4)
ScaI         2028  AGTACT               AGT^ACT
SexAI        2529  ACCAGGT              A^CC[AT]GGT
SfiI         1282  GGCCCTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI          51  TGTACA               T^GTACA
SspI         1000  AATATT               AAT^ATT
Tsp509I      4120  AATT                 ^AATT
XhoI         2643  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AflIII         30  ACGTGT               A^C[GA][CT]GT
             3518  ACGTGT              
Alw44I       1882  GTGCAC               G^TGCAC
             2816  GTGCAC              
ApaLI        1882  GTGCAC               G^TGCAC
             2816  GTGCAC              
BcgI         1162  CGAAGTGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2808  CGAGCCTCGTGC        
BsaAI          29  TACGTG               [CT]AC^GT[GA]
             3517  CACGTG              
BsmI         2303  GCATTC               GAATGC(1/-1)
             2845  GCATTC              
BstXI        1563  CCATGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3733  CCACTTTCATGG        
KspI          247  CCGCGG               CCGC^GG
             2370  CCGCGG              
NaeI         1957  GCCGGC               GCC^GGC
             3781  GCCGGC              
NgoMIV       1957  GCCGGC               G^CCGGC
             3781  GCCGGC              
SacI         1633  GAGCTC               GAGCT^C
             3396  GAGCTC              
SacII         247  CCGCGG               CCGC^GG
             2370  CCGCGG              


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        Asp700I     
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        XmnI